Skip to main content

Advertisement

SpringerLink
Log in

Effect of silver diamine fluoride on adhesion and microleakage of a pit and fissure sealant to tooth enamel: in vitro trial

  • Original Article
  • Published:
European Archives of Paediatric Dentistry Aims and scope Submit manuscript

Abstract

Aim

To identify the effect of silver diamine fluoride in adhesion and microleakage of a pit and fissure sealant to tooth enamel.

Methods

An in vitro study including 120 human molars without caries, and caries grade 1 was performed. Molars were randomly divided into four groups. Occlusal surfaces of groups 2 and 4 were sealed. In groups 1 and 3 silver diamine fluoride (SDF) was placed in the occlusal surface and then molars were sealed according to manufacturer’s instructions. Afterwards, all groups were submitted to the adhesion test. Groups 1 and 2 were placed in a forming cylinder and their roots were submerged in polymethyl methacrylate after that they were stored in deionised water (37 °C ± 1). Subsequently, they were tested by applying a tractional load (Instron machine, speed cross of 1 mm/min). Sealant adhesion was evaluated through: Resistance to the detachment (MPa), residues of sealant area (mm2) and adhesive remaining index (ARI) (0–3 interval). Microleakage test: Groups 3 and 4 were placed in a plastic container and subjected to 2000 cycles at different temperatures one at 6 °C ± 1 and the other at 60 °C ± 1. Later the occlusal surface was immersed for 24 h in 1% toluidine blue. Microleakage was observed by optical microscopy (10×) and was interpreted in micrometres.

Results

Of the samples without SDF 18.1 and 23.0% of the sealant remaining and of the samples with SDF present 100% of sealant remaining on enamel. Samples with SDF obtained an average in the test of detachment of 6.89 ± 3.09 MPa, whereas in the group without SDF the average obtained was 6.42 ± 3.35 MPa. Differences were not statistically significant (p = 0.579). Most of the samples not treated with SDF (81.6%) showed microleakage than those treated with SDF in which 47% presented microleakage.

Conclusions

There is an improvement in the retention properties of a fissure sealant applied after treatment with silver diamine fluoride. The application of fissure sealant to improve the aesthetics of teeth treated with SDF is recommended.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Baroudi K, Rodrigues J. Flowable resin composites: a systemic review and clinical considerations. J Clin Diagn Res. 2015;9:ZE18–24.

    PubMed  PubMed Central  Google Scholar 

  • Borsatto M, Yamamoto M, Gimatei M, et al. Bonding agent underneath sealant: shear bond strength to oil-contaminated enamel. Braz Den J. 2010;21:50–4.

    Article  Google Scholar 

  • Chu C-H, Lee AH, Zheng L, et al. Arresting rampant dental caries with silver diamine fluoride in young teenager suffering from chronic oral graft versus host disease post-bone marrow transplantation: a case report. BMC Res Notes. 2014;7:3.

    Article  Google Scholar 

  • Clu C, Lo E. Promoting caries arrest in children with silver diamine fluoride. Oral Health Prev Dent. 2015;6:315–21.

    Google Scholar 

  • Craig G, Knight G. Clinical evaluation of silver fluoride/ potassium iodide as a dentine desensitizing agent. A pilot study. Aust Dent J. 2012;57:308–11.

    Article  Google Scholar 

  • dos Santos VE, de Vasconcelos F, Ribeiro AG, et al. Paradigm Shift in the effective treatment of caries in school children at risk. Int Dent J. 2012;62:47–51.

    Article  Google Scholar 

  • Futatsuky M, Kubota K, Yeh YC, et al. Early loss of pit and fissure sealant: a clinical and SEM study. J Clin Pediatr Dent. 1995;19:99–104.

    Google Scholar 

  • Gao SS, Zhao IS, Hiraishi N, et al. Clinical trials of silver diamine fluoride in arresting caries among children: a systematic review. JDR Clin Transl Res. 2016;1:201–10.

    Article  Google Scholar 

  • Gotjamanos T, Alfonso F. Unacceptably high levels of fluoride in commercial preparations of silver fluoride. Aust Dent J. 1997;42:52–3.

    Article  Google Scholar 

  • Horst JA, Ellenikiotis H, Milgrom PL. UCSF Protocol for caries arrest using silver diamine fluoride: rationale, indications and consent. J Calif Dent Assoc. 2016;44(1):16–28.

    PubMed  PubMed Central  Google Scholar 

  • Joshi K, Dave B, Joshi N, et al. Comparative evaluation of two different pit and fissure sealants and a restorative material to check their microleakage- an in vitro study. J Int Oral Heath. 2013;5:35–9.

    Google Scholar 

  • Kassebaum N, Bernabé E, Dahiya M, et al. Global burden of untreated caries: a systematic review and metaregression. J Den Res. 2015;94:650–8.

    Article  Google Scholar 

  • Kenneth J. Anusavice. Ciencia de los materiales dentales. Madrid: Elsever; 2004. pp. 53–5.

    Google Scholar 

  • Komatsu H, Shimokobe H, Kawakami S, et al. Caries preventive effect of glass ionomer sealant reapplication; Study presents three-year results. J Am Dent Assoc. 1994;125:543–9.

    Article  Google Scholar 

  • Mei M, Chu C, Lo E. Fluoride and silver concentrations of silver diamine fluoride solutions for dental use. Int J Paediatr Dent. 2013a;23:279–85. https://doi.org/10.1111/ipd.12005.

    Article  PubMed  Google Scholar 

  • Mei M, Chu C, Low K, et al. Caries arresting effect of silver diamine fluoride on dentine carious lesion with S. mutans and L. acidophilus dual-species cariogenic biofilm. Med Oral Patol Oral Cir Bucal. 2013b;1;18:e824–31.

    Article  Google Scholar 

  • Mei ML, Ito L, Cao Y, et al. Inhibitory effect of silver diamine fluoride on dentine demineralisation and collagen degradation. J Dent. 2013c;41(9):809–17. https://doi.org/10.1016/j.jdent.2013.06.009.

    Article  PubMed  Google Scholar 

  • Mei M, Li Q, Chu C, et al. Antibacterial effects of silver diamine fluoride on multi-species cariogenic biofilm on caries. Ann Clin Microbiol Antimicrob. 2013d;26:12:4.

    Article  Google Scholar 

  • Mei M, Ito L, Cao Y, et al. The inhibitory effects of silver diamine fluoride on cysteine cathepsins. J Dent. 2014;42(3):329–35. https://doi.org/10.1016/j.jdent.2013.11.018.

    Article  PubMed  Google Scholar 

  • Monse B, Heinrich- Weltzien R, Mulder J, et al. Caries preventive efficacy of silver diamine fluoride (SDF) and ART sealants in a school-based daily fluoride toothbrushing program in the Philippines. BMC Oral Health. 2012;21:12:52. https://doi.org/10.1186/1472-6831-12-52.

    Article  Google Scholar 

  • Morales E, Martinez A, Hernández J, et al. Evaluation of marginal seal and microleakage of a sealant modified with silver nanoparticles in primary molars in vitro study. ODOVTOS. Int J Dent. 2014;16:107–13. https://doi.org/10.15517/ijds.v0i16.20329.

    Article  Google Scholar 

  • Nantanee R, Santiwong B, Trairatvoeakul C, et al. Silver diamine fluoride and glass ionomer differentially remineralize early caries lesions in situ. Clin Oral Investig. 2016;20(6):1151–7. https://doi.org/10.1007/s00784-015-1603-4.

    Article  PubMed  Google Scholar 

  • O´Brien KD, Watts DC, Read MJ. Residual debris and bond strength- is there a relationship. Am J Orthod Dentofac Orthop. 1988;94:222–30.

    Article  Google Scholar 

  • Peg J, Botelho M, Matinlinna J. Silver compounds used in dentistry for caries management: a review. J Dent. 2012;40:531–41.

    Article  Google Scholar 

  • Quock RL, Barros JA, Yang SW, et al. Effect of silver diamine fluoride on microtensile bond strength to dentin. Oper Dent. 2012;37:610–6.

    Article  Google Scholar 

  • Rosenblatt A, Stamford TCM, Niederman R. Silver diamine fluoride: a caries “silver-fluoride bullet”. J Dent Res. 2009;88(2):116–25.

    Article  Google Scholar 

  • Shama G, Puranik MP. KRS. Approaches to arresting dental caries: an update. J Clin Diagn Res. 2015;9:8–11.

    Google Scholar 

  • Soeno K, Taira Y, Matsumura H. Effect of desensitizers on bond strength of adhesive luting agents to dentin. J Oral Rehabilitation. 2001;28:1122–8.

    Article  Google Scholar 

  • Suzuki T, Nishida M, Sobue S, et al. Effects of diamine silver fluoride on tooth enamel. J Osaka Univ Dent Sch. 1974;14:61–72.

    PubMed  Google Scholar 

  • Uzuel I, Ulukent O, Cogulu D. The effect of silver diamine fluoride on microleakage of resin composite. J Int Dent Med Res. 2013;6:105–8.

    Google Scholar 

  • World Health Organization. Oral health surveys. Basic methods. 3rd. Suiza: Geneve; 1987.

    Google Scholar 

  • Wu D, Velamakanni S, Denisson J, et al. Effect of silver diamine fluoride application on microtensile bonding strength of dentin in primary teeth. Pediatr Dent. 2016;38:148–53.

    PubMed  Google Scholar 

  • Yee R, Holmgren C, Mulder J, et al. Efficacy of silver diamine fluoride for arresting caries treatment. J Dent Res. 2009;88:644–7.

    Article  Google Scholar 

  • Yeolekar T, Chowdhary N, Mukunda K, et al. Evaluation of microleakage and marginal ridge fracture resistence of primary molars restored with three restorative materials: a comparative study. Int J Clin Pediatr Dent. 2015;8:108–13.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. ENES Unidad León, Universidad Nacional Autónoma de México, Boulevard UNAM No 2011, Predio el Saucillo y el Potrero, 37684, León, Gto, Mexico

    J. Pérez-Hernández, F. C. Aguilar-Díaz, R. D. Venegas-Lancón, M. C. Villanueva-Vilchis & J. de la Fuente-Hernández

  2. Facultad de Odontología, Universidad Nacional Autónoma de México, Av Universidad 3000, Cd. Universitaria, Coyoacán, 04510, Mexico City, Mexico

    C. A. Á. Gayosso

Authors
  1. J. Pérez-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. C. Aguilar-Díaz
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. D. Venegas-Lancón
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. C. A. Á. Gayosso
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. M. C. Villanueva-Vilchis
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. J. de la Fuente-Hernández
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to F. C. Aguilar-Díaz.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Pérez-Hernández, J., Aguilar-Díaz, F.C., Venegas-Lancón, R.D. et al. Effect of silver diamine fluoride on adhesion and microleakage of a pit and fissure sealant to tooth enamel: in vitro trial. Eur Arch Paediatr Dent 19, 411–416 (2018). https://doi.org/10.1007/s40368-018-0374-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40368-018-0374-4

Keywords

Search

Navigation