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European Archives of Paediatric Dentistry

, Volume 18, Issue 6, pp 377–383 | Cite as

Tooth mineral density of different types of hypomineralised molars: a micro-CT analysis

  • C. Neboda
  • R. P. AnthonappaEmail author
  • N. M. King
Original Scientific Article

Abstract

Aim

This study sought to evaluate the tooth mineral density (TMD) for the different lesion types in hypomineralised first permanent molars (FPMs) and compare them to unaffected enamel in clinically sound FPMs.

Design

Eighteen FPMs with varying degrees of hypomineralised enamel were grouped into brown, yellow/creamy and white lesion types. Micro-CT was used to determine the TMD for each lesion type, and for unaffected enamel at different locations in the outer, middle, and inner-third of the enamel.

Results

The average TMD for brown, yellow/creamy, white and unaffected enamel was 1.79, 2.21, 2.43 and 2.46 g/cm3, respectively. Brown and yellow/creamy lesions exhibited a statistically significant difference when compared to white lesions and unaffected enamel. However, no statistical difference was evident in TMD between white lesions and unaffected enamel. The TMD increased from the outer-third to inner-third for brown and yellow/creamy lesions (p < 0.05), while in white lesions and unaffected enamel, the TMD decreased from the outer-third to inner-third (p < 0.05).

Conclusion

TMD was lowest for brown lesions followed by yellow/creamy lesions while the TMD for white lesions was similar to unaffected enamel.

Keywords

Hypomineralisation Tooth mineral density Permanent molars Molar-incisor hypomineralisation 

Notes

Acknowledgements

The authors acknowledge the facilities, and the scientific and technical assistance of the National Imaging Facility at the Centre for Microscopy, Characterisation and Analysis, and University of Western Australia, a facility funded by the University, State and Commonwealth Governments. The authors would also wish to sincerely thank the Australian Dental Research Foundation for its financial support of this research.

Compliance with ethical standards

Conflict of interest

The authors declare no conflict of interest.

Funding

This research is supported by the Australian Dental Research Fund.

References

  1. Americano GC, Jacobsen PE, Soviero VM, Haubek D. A systematic review on the association between molar incisor hypomineralization and dental caries. Int J Paediatr Dent. 2017;27:11–21.CrossRefPubMedGoogle Scholar
  2. Chay PL, Manton DJ, Palamara JEA. The effect of resin infiltration and oxidative pre-treatment on microshear bond strength of resin composite to hypomineralised enamel. Int J Paediatr Dent. 2014;24:252–67.CrossRefPubMedGoogle Scholar
  3. Clementino-Luedemann TN, Kunzelmann KH. Mineral concentration of natural human teeth by a commercial micro-CT. Dent Mater J. 2006;25:113–9.CrossRefPubMedGoogle Scholar
  4. Commission on Oral Health Report. A review of the developmental defects of enamel index (DDE Index). Int Dent J. 1992;42:411–26.Google Scholar
  5. Crombie FA, Cochrane NJ, Manton DJ, Palamara JEA, Reynolds EC. Mineralisation of developmentally hypomineralised human enamel in vitro. Caries Res. 2013;47:259–63.CrossRefPubMedGoogle Scholar
  6. Da Costa-Silva CM, Ambrosano GMB, Jeremias F, De Souza JF, Mialhe FL. Increase in severity of molar-incisor hypomineralization and its relationship with the colour of enamel opacity: a prospective cohort study. Int J Paediatr Dent. 2011;21:333–41.CrossRefPubMedGoogle Scholar
  7. Dowker SEP, Elliott JC, Davis GR, Wilson RM, Cloetens P. Three-dimensional study of human dental fissure enamel by synchrotron X-ray microtomography. Eur J Oral Sci. 2003;114:353–9.CrossRefGoogle Scholar
  8. Dowker SEP, Elliott JC, Davis GR, Wilson RM, Cloetens P. Synchrotron X-ray microtomographic investigation of mineral concentrations at micrometre scale in sound and carious enamel. Caries Res. 2004;38:514–22.CrossRefPubMedGoogle Scholar
  9. Elcock C, Lath DL, Luty JD, et al. The new Enamel Defect Index: testing and expansion. Eur J Oral Sci. 2006;114:35–8.CrossRefPubMedGoogle Scholar
  10. Fagrell TG, Lingstrom P, Olsson S, Steiniger F, Noren JG. Bacterial invasion of dentinal tubules beneath apparently intact but hypomineralized enamel in molar teeth with molar incisor hypomineralization. Int J Paediatr Dent. 2008;18:333–40.CrossRefPubMedGoogle Scholar
  11. Fagrell TG, Dietz W, Jälevik B, Noren JG. Chemical, mechanical and morphological properties of hypomineralised enamel of permanent first molars. Acta Odontol Scand. 2010;68:215–22.CrossRefPubMedGoogle Scholar
  12. Fagrell TG, Salmon P, Melin L, Noren JG. Onset of molar incisor hypomineralization (MIH). Swed Dent J. 2013;37:61–70.PubMedGoogle Scholar
  13. Farah RA, Swain MV, Drummond BK, Cook R, Atieh M. Mineral density of hypomineralised enamel. J Dent. 2010a;38:50–8.CrossRefPubMedGoogle Scholar
  14. Farah RA, Drummond BK, Swain MV, Williams S. Linking the clinical presentation of molar-incisor hypomineralisation to its mineral density. Int J Paediatr Dent. 2010b;36:915–21.Google Scholar
  15. Fearne J, Anderson P, Davis GR. 3D X-ray microscopic study of the extent of variations in enamel density in first permanent molars with idiopathic enamel hypomineralisation. Br Dent J. 2004;196:634–8.CrossRefPubMedGoogle Scholar
  16. Garot E, Rouas P, D’Incau E, et al. Mineral density of hypomineralised and sound enamel. Bull Group Int Rech Sci Stomatol Odontol. 2016;53:33–6.Google Scholar
  17. Ghanim A, Elfrink M, Weerheijm K, Marino R, Manton DJ. A practical method for use in epidemiological studies on enamel hypomineralisation. Eur Arch Paediatr Dent. 2015;16:235–46.CrossRefPubMedPubMedCentralGoogle Scholar
  18. Gill DS, Lee RT, Tredwin CJ. Treatment planning for the loss of first permanent molars. Dent Update. 2001;28:304–8.CrossRefPubMedGoogle Scholar
  19. He B, Huang S, Jing J, Hao Y. Measurement of hydroxyapatite density and Knoop hardness in sound human enamel and a correlational analysis between them. Arch Oral Biol. 2010;55:134–41.CrossRefPubMedGoogle Scholar
  20. He B, Huang S, Zhang C, et al. Mineral densities and elemental content in different layers of healthy human enamel with varying teeth age. Arch Oral Biol. 2011;56:997–1004.CrossRefPubMedGoogle Scholar
  21. Huang TTY, Jones AS, He LH, Ali Darendeliler M, Swain M. Characterisation of enamel white spot lesions using X-ray micro-tomography. J Dent. 2007;35:737–43.CrossRefPubMedGoogle Scholar
  22. Jälevik B, Noren JG. Enamel hypomineralization of permanent first molars: a morphological study and survey of possible aetiological factors. Int J Paediatr Dent. 2000;10:278–89.CrossRefPubMedGoogle Scholar
  23. Jälevik B, Klinberg G. A Dental treatment, dental fear and behaviour management problems in children with severe enamel hypomineralization of their permanent first molars. Int J Paediatr Dent. 2002;12:24–32.PubMedGoogle Scholar
  24. Jälevik B. Prevalence and diagnosis of molar incisor hypomineralisation (MIH): a systematic review. Eur Arch Paediatr Dent. 2010;11:59–64.CrossRefPubMedGoogle Scholar
  25. Koch G, Hallonsten AL, Ludvigsson N, et al. Epidemiologic study of idiopathic enamel hypomineralization in permanent teeth of Swedish children. Community Dent Oral Epidemiol. 1987;15:279–85.CrossRefPubMedGoogle Scholar
  26. Mahoney E, Ismail FSM, Kilpatrick N, Swain M. Mechanical properties across hypomineralised/hypoplastic enamel of first permanent molar teeth. Eur J Oral Sci. 2004;112:497–502.CrossRefPubMedGoogle Scholar
  27. Mathu-Muju K, Wright JT. Diagnosis and treatment of molar incisor hypomineralization. Compend Contin Educ Dent. 2006;27:604–10.PubMedGoogle Scholar
  28. Neves AA, Coutinho E, Cardoso MV, Jaecques SV, Meerbeek BV. Micro-CT based quantification evaluation of caries excavation. Dent Mater. 2010;26:579–88.CrossRefGoogle Scholar
  29. Oliver K, Messer LB, Manton DJ, et al. Distribution and severity of molar hypomineralisation: trial of a new severity index. Int Dent J. 2014;24:131–51.Google Scholar
  30. Rodd HD, Boissonade FM, Day PF. Pulpal status of hypomineralized permanent molars. Pediatr Dent. 2007;29:514–20.PubMedGoogle Scholar
  31. Schwass DR, Swain MV, Purton DG, Leichter JW. A system of calibrating microtomography for use in caries research. Caries Res. 2009;43:314–21.CrossRefPubMedGoogle Scholar
  32. Silva MJ, Scurrah KJ, Craig JM, Manton DJ, Kilpatrick N. Etiology of molar incisor hypomineralization—a systematic review. Community Dent Oral Epidemiol. 2016;44:342–53.CrossRefPubMedGoogle Scholar
  33. Weerheijm KL, Jälevik B, Alaluusua S. Molar-incisor hypomineralisation. Caries Res. 2001;35:390–1.CrossRefPubMedGoogle Scholar
  34. Weerheijm KL. Molar incisor hypomineralisation. Eur J Paediatr Dent. 2003;4:114–20.PubMedGoogle Scholar
  35. Weerheijm KL, Duggal M, Mejare I, et al. Judgement criteria for molar incisor hypomineralisation (MIH) in epidemiologic studies: a summary of the European meeting on MIH held in Athens, 2003. Eur Arch Paediatr Dent. 2006;4:110–3.Google Scholar

Copyright information

© European Academy of Paediatric Dentistry 2017

Authors and Affiliations

  1. 1.Paediatric Dentistry, School of DentistryThe University of Western AustraliaNedlandsAustralia

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