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Diversity of cultivable endophytic bacteria associated with halophytes in Xinjiang of China and their plant beneficial traits

Abstract

Endophytic bacteria from halophytes have a wide range of application prospects in various fields, such as plant growth-promoting, biocontrol activity and stress resistance. The current study aimed to identify cultivable endophytic bacteria associated with halophytes grown in the salt-affected soil in Xinjiang Uygur Autonomous Region, China and to evaluate their plant beneficial traits and enzyme-producing activity. Endophytic bacteria were isolated from Reaumuria soongorica (PalL Maxim.), Artemisia carvifolia (Buch.-Ham. ex Roxb. Hort. Beng.), Peganum harmala L. and Suaeda dendroides (C. A. Mey. Moq.) by using the cultural-dependent method. Then we classified these bacteria based on the difference between their sequences of 16S rRNA (16S ribosomal RNA) gene. Results showed that the isolated bacteria from R. soongorica belonged to the genera Brucella, Bacillus and Variovorax. The bacteria from A. carvifolia belonged to the genera Micromonospora and Brucella. The bacteria from P. harmala belonged to the genera Paramesorhizobium, Bacillus and Peribacillus. The bacteria from S. dendroides belonged to the genus Bacillus. Notably, the genus Bacillus was detected in the three above plants, indicating that Bacillus is a common taxon of endophytic bacteria in halophytes. And, our results found that about 37.50% of the tested strains showed strong protease-producing activity, 6.25% of the tested strains showed strong cellulase-producing activity and 12.50% of the tested strains showed moderate lipase-producing activity. Besides, all isolated strains were positive for IAA (3-Indoleacetic acid) production, 31.25% of isolated strains exhibited a moderate phosphate solubilization activity and 50.00% of isolated strains exhibited a weak siderophore production activity. Our findings suggest that halophytes are valuable resources for identifying microbes with the ability to increase host plant growth and health in salt-affected soils.

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Acknowledgements

This research was supported by the Xinjiang Uygur Autonomous Region Regional Coordinated Innovation Project (Shanghai Cooperation Organization Science and Technology Partnership Program) (2020E01047), and the National Natural Science Foundation of China (U1703106, 32061143043, 91751206). The authors acknowledge the anonymous reviewers for their constructive comments on the manuscript.

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Correspondence to Wenjun Li or Jinbiao Ma.

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Li, L., Gao, L., Liu, Y. et al. Diversity of cultivable endophytic bacteria associated with halophytes in Xinjiang of China and their plant beneficial traits. J. Arid Land 13, 790–800 (2021). https://doi.org/10.1007/s40333-021-0016-2

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  • DOI: https://doi.org/10.1007/s40333-021-0016-2

Keywords

  • endophytes
  • environmental microbiology
  • halophytes
  • biodiversity
  • plant beneficial properties