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Endophytic bacteria associated with halophyte Seidlitzia rosmarinus Ehrenb. ex Boiss. from saline soil of Uzbekistan and their plant beneficial traits

Journal of Arid Land volume 12, pages 730–740 (2020)Cite this article

Abstract

Endophytic bacteria of halophytic plants play essential roles in salt stress tolerance. Therefore, an understanding of the true nature of plant—microbe interactions under extreme conditions is essential. The current study aimed to identify cultivable endophytic bacteria associated with the roots and shoots of Seidlitzia rosmarinus Ehrenb. ex Boiss. grown in the salt-affected soil in Uzbekistan and to evaluate their plant beneficial traits related to plant growth stimulation and stress tolerance. Bacteria were isolated from the roots and the shoots of S. rosmarinus using culture-dependent techniques and identified by the 16S rRNA gene. RFLP (Restriction Fragment Length Polymorphism) analysis was conducted to eliminate similar isolates. Results showed that the isolates from the roots of S. rosmarinus belonged to the genera Rothia, Kocuria, Pseudomonas, Staphylococcus, Paenibacillus and Brevibacterium. The bacterial isolates from the shoots of S. rosmarinus belonged to the genera Staphylococcus, Rothia, Stenotrophomonas, Brevibacterium, Halomonas, Planococcus, Planomicrobium and Pseudomonas, which differed from those of the roots. Notably, Staphylococcus, Rothia and Brevibacterium were detected in both roots and shoots, indicating possible migration of some species from roots to shoots. The root-associated bacteria showed higher levels of IAA (indole-3-acetic acid) synthesis compared with those isolated from the shoots, as well as the higher production of ACC (1-aminocyclopropane-1-carboxylate) deaminase. Our findings suggest that halophytic plants are valuable sources for the selection of microbes with a potential to improve plant fitness under saline soils.

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Acknowledgements

This research was supported by the Eurasia Program of the Norwegian Centre for Cooperation in Education (CPEA-LT-2016/10095), the German Academic Exchange Service (DAAD) and the President’s International Fellowship Initiative of the Chinese Academy of Sciences (2018VBA002S).

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Authors and Affiliations

  1. Faculty of Biology, National University of Uzbekistan, Tashkent, 100174, Uzbekistan

    Vyacheslav Shurigin

  2. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi, 830011, China

    Dilfuza Egamberdieva & Li Li

  3. Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, 15374, Germany

    Dilfuza Egamberdieva, Stephan Wirth & Sonoko Dorothea Bellingrath-Kimura

  4. Institute of Microbiology of the Academy of Sciences of the Republic of Uzbekistan, Tashkent, 100128, Uzbekistan

    Kakhramon Davranov

  5. Department of Biochemistry, Microbiology and Biotechnology, Yerevan State University, Yerevan, 0025, Armenia

    Hovik Panosyan

  6. Department of Biological Sciences, University of Bergen, NO-5020, Bergen, 7803, Norway

    Nils-Kåre Birkeland

  7. Faculty of Life Science, Humboldt University of Berlin, Berlin, 14195, Germany

    Sonoko Dorothea Bellingrath-Kimura

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  1. Vyacheslav Shurigin
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Shurigin, V., Egamberdieva, D., Li, L. et al. Endophytic bacteria associated with halophyte Seidlitzia rosmarinus Ehrenb. ex Boiss. from saline soil of Uzbekistan and their plant beneficial traits. J. Arid Land 12, 730–740 (2020). https://doi.org/10.1007/s40333-020-0019-4

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Keywords

  • endophytic bacteria
  • phylogenetic analysis
  • halophyte
  • auxin
  • plant beneficial traits