Sports Medicine

, Volume 47, Issue 4, pp 781–789 | Cite as

Utility of S100B Serum Level for the Determination of Concussion in Male Rugby Players

  • Damien Bouvier
  • Thomas Duret
  • Mathieu Abbot
  • Thibault Stiernon
  • Bruno Pereira
  • Aurélien Coste
  • Jean Chazal
  • Vincent SapinEmail author
Original Research Article
Part of the following topical collections:
  1. Rugby Health



The incidence of concussion in professional direct-contact sports, particularly in rugby, has increased in recent years. To date, cognitive assessment is the most common means of determining whether a concussed player can return to the game. Serum S100B assay, an objective blood test known to be useful in brain injury management, may offer a novel additional approach to the management of concussed male rugby players.


The aim of this study was to investigate the S100B utility for the determination of concussion in a professional 15-players-a-side rugby team.


Thirty-nine male rugby players were included in a prospective study during the 2014–2015 French championship season. Serum sampling was carried out several times at baseline and after a match and/or a concussion, at set times (2, 36 h). Serum S100B concentrations were determined using chemiluminescence immunoassay on a Roche Diagnostics® instrument.


The players’ basal serum S100B was stable during the season and was not correlated with anthropometric data, body composition, or creatine kinase concentration. A significant increase in S100B concentration within 2 h after a game (without concussion) was observed. This increase was correlated with the number of body collisions during a match. Seventy-seven assays were performed 36 h after a game, including the follow-up of five concussed players. Thirty-six hours after a match, an increase of a minimum of 20 % compared with individual basal concentrations identified concussion with 100 % sensitivity and 81 % specificity.


S100B measured 36 h after a match is thus a discriminating test to identify concussion in a male rugby player, with a 100 % negative predictive value.


Mild Traumatic Brain Injury Creatine Kinase Activity Chronic Traumatic Encephalopathy Basal Concentration S100B Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank the biologists of Genbio® Laboratory for their help in blood sampling for the S100B basal determination taken on three occasions; and the Auvergne Traduction Technique (ATT) society for proofreading and editing this manuscript.

Compliance with Ethical Standards

Conflict of interest

Damien Bouvier, Thomas Duret, Thibault Stiernon, Bruno Pereira, Mathieu Abbot, Aurélien Coste, Jean Chazal, and Vincent Sapin declare that they have no conflicts of interest.

Ethical approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.

Informed consent

Informed consent was obtained from all individual participants included in the study.


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Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Damien Bouvier
    • 1
    • 2
  • Thomas Duret
    • 1
  • Mathieu Abbot
    • 3
    • 4
  • Thibault Stiernon
    • 3
  • Bruno Pereira
    • 5
  • Aurélien Coste
    • 6
  • Jean Chazal
    • 6
  • Vincent Sapin
    • 1
    • 2
    • 7
    • 8
    Email author
  1. 1.Department of Biochemistry and Molecular BiologyCHU Clermont-FerrandClermont-FerrandFrance
  2. 2.EA7281-Retinoids, Reproduction Developmental Diseases, School of MedicineClermont Université, Université d’AuvergneClermont-FerrandFrance
  3. 3.Medical DepartmentSports Association Montferrandaise Clermont Auvergne (ASMCA)Clermont-FerrandFrance
  4. 4.Department of Sport Medicine and Functional ExplorationsCHU Clermont-FerrandClermont-FerrandFrance
  5. 5.Department of Biostatistics Unit (DRCI)CHU Clermont-FerrandClermont-FerrandFrance
  6. 6.Department of NeurosurgeryCHU Clermont-FerrandClermont-FerrandFrance
  7. 7.Laboratoire de Biochimie Médicale, 4R3, Faculté de MédecineUniversité d’AuvergneClermont Ferrand CedexFrance
  8. 8.Observatoire Médical, FFR-LNRMarcoussisFrance

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