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Electrochemical Analysis of Single Glucose Oxidase with a Nanopipette

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Chemical Research in Chinese Universities Aims and scope

Abstract

Nanopore-based electrochemical technique is a promising tool for detecting single proteins. However, detecting single proteins using a nanopipette in their native state without labeling is challenging due to the rapid translocation, which results in an inefficient signal identification. In our study, we finely tuned the driving force equilibrium between electrophoretic force (EPF) and electroosmotic flow (EOF) inside the nanopipette for efficient sensing of single glucose oxidase (GOD) molecules. The duration time of GOD within the nanopipette is extended to about 4 ms. This strategy provided clear ionic current signals with a signal-to-noise ratio of 3.3. As EPF increased in the direction opposite to the motion of GOD, we observed a nonlinear growth in GOD’s duration time. This extended the duration to about 4.4 times longer at −1000 mV compared to at −800 mV. Hence, nanopore-based electrochemical sensing could be used for single GOD molecule analysis as an ultrasensitive method.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 22104052, 2233000271, 22027806).

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Molecular Sensing and Imaging Center (MSIC), Nanjing University, Nanjing, 210023, P. R. China

    Yongyong Wang, Hui Ma, Yi-Tao Long & Yi-Lun Ying

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  1. Yongyong Wang
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  2. Hui Ma
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  3. Yi-Tao Long
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  4. Yi-Lun Ying
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Corresponding authors

Correspondence to Hui Ma or Yi-Lun Ying.

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LONG Yi-Tao is an editorial board member for Chemical Research in Chinese Universities and was not involved in the editorial review or the decision to publish this article. The authors declare no conflicts of interest.

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Wang, Y., Ma, H., Long, YT. et al. Electrochemical Analysis of Single Glucose Oxidase with a Nanopipette. Chem. Res. Chin. Univ. 40, 272–278 (2024). https://doi.org/10.1007/s40242-024-3281-8

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  • DOI: https://doi.org/10.1007/s40242-024-3281-8

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