Profiling of Ubiquitination Modification Sites in Talin in Colorectal Carcinoma by Mass Spectrometry

Abstract

Talin protein was partially purified from human colorectal carcinoma tissues, which was subject to tryptic digestion. Immunoaffinity precipitation with specific antibodies that recognize diglycyl-lysine(Lys) remnants from tryptic digestion of ubiquitinated peptides was used to enrich ubiquitinated sites in talin. Mass spectrometry coupled with capillary reverse-phase high-performance liquid chromatography was used to analyze the enriched peptides. Specifically, four peptides containing diglycyl-Lys remnants from talin, namely, TAK(ub)VLVEDTK, QQQYK(ub) FLPSELRDEH, K(ub)STVLQQQYNR, and EGILK(ub)TAK can be determined using mass spectrometric data. This study provides an analytical method for further study in the relationship between ubiquitination modification of talin and its biological activity in colorectal cancer tissues with different pathological processes.

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References

  1. [1]

    Zhang Y., Chen Z., Li J., Medicine(Baltimore), 2017, 96, e8242

    Google Scholar 

  2. [2]

    Arnold M., Sierra M. S., Laversanne M., Soerjomataram I., Jemal A., Bray F., Gut, 2017, 66, 683

    Article  PubMed  PubMed Central  Google Scholar 

  3. [3]

    AI Bandar M. H., Kim N. K., Oncol. Rep., 2017, 37, 2553

    Article  CAS  Google Scholar 

  4. [4]

    Balacescu O., Sur D., Cainap C., Visan S., Cruceriu D., Man-zat-Saplacan R., Muresan M. S., Balacescu L., Lisencu C., Irimie A., Int. J. Mol. Sci., 2018, 19(12), 3711

    Article  CAS  PubMed Central  Google Scholar 

  5. [5]

    Desiniotis A., Kyprianou N., Int. Rev. Cell. Mol. Biol., 2011, 289, 117

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. [6]

    Yang H. J., Chen J. Z., Zhang W. L., Ding Y. Q., Cancer Invest., 2010, 28(2), 127

    Article  CAS  PubMed  Google Scholar 

  7. [7]

    Chinthalapudi K., Rangarajan E. S., Izard T., Proc. Natl. Acad. Sci. USA, 2018, 115, 10339

    Article  CAS  PubMed  Google Scholar 

  8. [8]

    Huang Z., Barker D., Gibbins J. M., Dash P. R., Exp. Cell Res., 2018, 370, 417

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. [9]

    Yates M., Maréchal A., Int. J. Mol. Sci., 2018, 19(10), 2909

    Article  CAS  PubMed Central  Google Scholar 

  10. [10]

    Abreha M. H., Dammer E. B., Ping L., Zhang T., Duong D. M., Gearing M., Lah J. J., Levey A. I., Seyfried N. T., Proteomics, 2018, 78, e1800108

    Article  CAS  Google Scholar 

  11. [11]

    Peng Q. S., Li G. P., Sun W. C., Yang J. B., Quan G. H., Liu N., Chinese Journal of Analytical Chemistry, 2016, 44, 850

    Article  CAS  Google Scholar 

  12. [12]

    Huang C., Rajfur Z., Yousefi N., Chen Z., Jacobson K., Ginsberg M. H., Nat. Cell Biol., 2009, 11 624

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. [13]

    Liu N., Song W., Wang P., Lee K. C., Cai Z., Chen H., Proteomics, 2010, 10, 1875

    Article  CAS  PubMed  Google Scholar 

  14. [14]

    Liu N., Song W., Lee KC., Wang P., Chen H., Cai Z., J. Am. Soc. Mass Spectrom., 2009, 20, 312

    Article  CAS  PubMed  Google Scholar 

  15. [15]

    Cheng W., Zhan F. L., Li S. M., Yang J. B., Wang Y., Liu N., Chinese Journal of Analytical Chemistry, 2019, 47, 30

    Article  CAS  Google Scholar 

  16. [16]

    Murphy S., Zweyer M., Henry M., Meleady P., Mundegar R. R., Swandulla D., Ohlendieck K., Clin. Proteomics, 2018, 75, 34

    Article  CAS  Google Scholar 

  17. [17]

    Yang G. Y., Zhao H. F., Xie F., Li Y., Sun L., Shi Q. H., He C. Y., Liu N., Song L. N., Chem. Res. Chinese Universities, 2012, 28(6), 1031

    Google Scholar 

  18. [18]

    Yang H., Su R., Wishnok J. S., Liu N., Chen C., Liu S., Tannenbaum S. R., Mikrochim. Acta, 2019, 786, 104

    Article  CAS  Google Scholar 

  19. [19]

    Liu N., Zhao L. C., He C. Y., Cai Z. W., Curr. Anal. Chem., 2012, 8, 22

    Article  CAS  Google Scholar 

  20. [20]

    Feng S., Pan C., Jiang X., Xu S., Zhou H., Ye M., Zou H., Proteomics, 2007, 7, 351

    Article  CAS  PubMed  Google Scholar 

  21. [21]

    Pascovici D., Wu J. X., McKay M. J., Joseph C., Noor Z., Kamath K., Wu Y., Ranganathan S., Gupta V., Mirzaei M., Int. J. Mol. Sci., 2019, 20(1), 16

    Article  CAS  Google Scholar 

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Correspondence to Yimin Wang or Xuesong Xu.

Additional information

Supported by the Fund of Science and Technology Department of Jilin Province, China(No.20150414015GH) and the National Natural Science Foundation of China(Nos.81572082, 81472454).

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Wang, K., Qiao, L., Li, X. et al. Profiling of Ubiquitination Modification Sites in Talin in Colorectal Carcinoma by Mass Spectrometry. Chem. Res. Chin. Univ. 35, 377–381 (2019). https://doi.org/10.1007/s40242-019-8377-1

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Keywords

  • Talin
  • Ubiquitination
  • Colorectal carcinoma
  • Mass spectrometry