Skip to main content
SpringerLink
Log in

A Label-free and Functional Fluorescent Oligonucleotide Probe Based on a G-Quadruplex Molecular Beacon for the Detection of Kanamycin

  • Published:
Chemical Research in Chinese Universities Aims and scope

Abstract

A label-free and turn-off fluorescent method for the quantitative detection of kanamycin based on a functional molecular beacon was developed. The molecular beacon consists of two hairpin structures with a split G-rich oligonucleotide in the middle. The kanamycin’s aptamer formed the loops portion for recognizing kanamycin, and the G-quadruplex bound by Thioflavin T(ThT) was employed as the reporter. In the absence of target, the molecular beacon folded into double stem-loops and the splited G-rich oligonucleotid came close to form a G-quadruplex. When ThT bound to the G-quadruplex, the fluorescence intensity of the solution increased. Upon the addition of kanamycin, the function between kanamycin and aptamer unfolded the hairpin and disassembled the G-quadraplex structure, resulting in a significant decrease in the fluorescence intensity. A good linear relationship ranging from 0.7 nmol/L to 10 nmol/L was achieved and the limit of detection was 0.37 nmol/L. Besides, it could efficiently recognize kanamycin in real samples.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Megoulas N. C., Koupparis M. A., Anal. Chim. Acta, 2005, 547, 64

    Article  CAS  Google Scholar 

  2. Cox L. A., Clin. Infect. Dis., 2006, 42, 1053

    Article  PubMed  Google Scholar 

  3. Hurd H. S., Enoe C., Sorensen L., Wachman H., Corns S. M., Bryden K. M., Grenier M., Risk Anal., 2008, 28, 695

    Article  PubMed  Google Scholar 

  4. Phillips I., Casewell M., Cox T., de Groot B., Friis C., Jones R., Nightingale C., Preston R., Waddell J., J. Antimicrob. Chemother., 2004, 54, 276

    Article  CAS  Google Scholar 

  5. Phillips I., Casewell M., Cox T., de Groot B., Friis C., Jones R., Nightingale C., Preston R., Waddell J., J. Antimicrob. Chemother., 2004, 53, 28

    Article  CAS  PubMed  Google Scholar 

  6. Oertel R.Neumeister, Kirch W. V., J. Chromatogr. A, 2004, 1058, 197

    Article  CAS  PubMed  Google Scholar 

  7. Kitasato I., Yokota M., Inouye S., Igarashi M., Chemotherapy, 1990, 36, 155

    Article  CAS  PubMed  Google Scholar 

  8. Xing Y. P., Liu C., Zhou X. H., Sci. Rep.-UK, 2015, 5,8125

    Article  CAS  Google Scholar 

  9. Chen S. H., Liang Y. C., Chou Y. W., J. Sep. Sci., 2006, 29, 607

    Article  CAS  PubMed  Google Scholar 

  10. Wang L. F., Peng J. D., Chromatographia, 2009, 69, 519

    Article  CAS  Google Scholar 

  11. Blanchaert B., Jorge E. P., Jankovics P., Adams E., Ann V. S., Chro-matographia, 2013, 76, 1505

    Article  CAS  Google Scholar 

  12. Long Y. H., Hernandez M., Kaale E., van Schepdael R. A., E., Bor-rull F., Calull M., Hoogmartens J., J. Chromatogr., 2003, 784, 255

    CAS  Google Scholar 

  13. Kaale E., Schepdael A.V., Roets E., Hoogmartens J., Electrophoresis, 2003, 24, 1119

    Article  CAS  PubMed  Google Scholar 

  14. Isoherranen N., Soback S., J. AOAC. Int., 1999, 82, 1017

    CAS  PubMed  Google Scholar 

  15. Wang X. Y., Zou M. J., Xu X., Lei R., Li K., Li N., Anal. Bioanal. Chem., 2009, 395, 2397

    Article  CAS  PubMed  Google Scholar 

  16. Raz S. R., Bremer M. G. E. G., Haasnoot W., Norde W., Anal. Chem., 2009, 81, 7743

    Article  CAS  Google Scholar 

  17. Frasconi M., Tel-Vered R., Riskin M., Willner I., Anal. Chem., 2010, 82, 2512

    Article  CAS  PubMed  Google Scholar 

  18. Althaus R., Berruga M. I., Montero A., Roca M., Molina M. P., Anal. Chim. Acta, 2009, 632, 159

    Article  CAS  Google Scholar 

  19. Chen Y. Q., Wang Z. Q., Wang Z. H., Tang S. S., Zhu Y., Xiao X. L., J. Agric. Food Chem., 2008, 56, 2944

    Article  CAS  PubMed  Google Scholar 

  20. Loomans E. E. M. G., Wiltenburg J. V., Koets. M, Amerongen A. V., J. Agric., Food Chem., 2003, 51, 587

    Article  CAS  Google Scholar 

  21. Tyagi S., Kramer N. F. R., Biotechnol., 1996, 14, 303

    CAS  Google Scholar 

  22. Morrison L. E., Halder T. C., Stols L. M., Anal. Biochem., 1989, 183, 231

    Article  CAS  PubMed  Google Scholar 

  23. Liu B., Yang X., Wang K., Chem. Res. Chinese Universities, 2012, 28(1), 37

    Google Scholar 

  24. Kostrikis L. G., Tyagi S., Mhlanga M. M., Ho D. D., Kramer F. R., Science, 1998, 279, 1228

    Article  CAS  PubMed  Google Scholar 

  25. Tyagi S., Bratu D. P., Kramer F. R., Nat. Biotechnol., 1998, 16, 49

    Article  CAS  PubMed  Google Scholar 

  26. Perlette J., Tan W., Anal. Chem., 2001, 73, 5544

    Article  CAS  PubMed  Google Scholar 

  27. Fang X., Li J. J., Tan W., Anal. Chem., 2000, 72, 3280

    Article  CAS  PubMed  Google Scholar 

  28. Li J., Tan W., Wang K., Xiao D., Yang X., He X., Tang Z., Anal. Sci., 2001, 17, 1149

    Article  CAS  PubMed  Google Scholar 

  29. Tang Z., Wang K., Tan W., Li J., Liu L., Guo Q., Meng X., Ma C., Huang S., Nucleic Acids Res., 2003, 31, e148

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  30. Li J. J., Geyer R., Tan W., Nucleic Acids Res., 2000, 28, e52

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Bourdoncle A., Estevez T. A., Gosse C., Lacroix L., Vekhoff P., Le S. T., Jullien L., Mergny J. L., J. Am. Chem. Soc., 2006, 128, 11094

    Article  CAS  PubMed  Google Scholar 

  32. Williamson J. R., Annu. Rev. Biophys. Biomol. Struct., 1994, 23, 703

    Article  CAS  PubMed  Google Scholar 

  33. Sundquist W. I., Klug A., Nature, 1989, 342, 825

    Article  CAS  PubMed  Google Scholar 

  34. Song K. M., Cho M., Jo H., Min K., Jeon S. H., Kim T., Han M. S., Ku J. K., Ban C., Anal. Biochem., 2011, 415, 175

    Article  CAS  PubMed  Google Scholar 

  35. Chen T. X., Ning F., Liu H. S., Wu K. F., Li W., Chinese Chem. Lett., 2017, 28, 1380

    Article  CAS  Google Scholar 

  36. Tong L. L., Li L., Chen Z., Biosens. Bioelectro., 2013, 49, 420

    Article  CAS  Google Scholar 

  37. Wen Y., Wang L., Li L., Sensors, 2016, 16, 12

    Google Scholar 

  38. Stoltenburg R., Nikolaus N., Strehlitz B., J. Anal. Methods Chem., 2012, 14, 383

    Google Scholar 

  39. Bhasikuttan A. C., Mohanty J., Chem. Commun., 2015, 51, 7581

    Article  CAS  Google Scholar 

  40. Tong L., Li L., Chen Z., Biosens. Bioelectron, 2013, 49, 420

    Article  CAS  PubMed  Google Scholar 

  41. Nikolaus N., Strehlitz B., Sensors, 2014, 14, 37

    Article  CAS  Google Scholar 

  42. Amdursky N., Erez Y., Dan H. H., Acc. Chem. Res., 2012, 45, 1548

    Article  CAS  PubMed  Google Scholar 

  43. Mohanty J., Barooah N., Dhamodharan V., J. Am. Chem. Soc., 2013, 135, 367

    Article  CAS  PubMed  Google Scholar 

  44. Green J., Ying L., Klenerman D., Chem. Res. Chinese Universities, 2002, 18(1), 103

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Chemistry and Biological Engineering, Changsha University of Science and Technology, Changsha, 410004, P. R. China

    Yuqing Zhu, Wei Li, Suzhen Tan & Tianxiao Chen

Authors
  1. Yuqing Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Wei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Suzhen Tan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Tianxiao Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Wei Li or Suzhen Tan.

Additional information

Supported by the National Natural Science Foundation of China(No.21202010), the Natural Science Foundation of Hunan Province, China(No.2017JJ2275) and the Scientific Research Fund of Hunan Provincial Education Department, China (Nos.16B003, 17C0033).

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Zhu, Y., Li, W., Tan, S. et al. A Label-free and Functional Fluorescent Oligonucleotide Probe Based on a G-Quadruplex Molecular Beacon for the Detection of Kanamycin. Chem. Res. Chin. Univ. 34, 541–545 (2018). https://doi.org/10.1007/s40242-018-7366-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40242-018-7366-0

Keywords

Search

Navigation