Chemical Research in Chinese Universities

, Volume 34, Issue 2, pp 229–234 | Cite as

Detection of Intracellular Proteins and Biomarkers Using Modified Silica Nanoparticles and Flow Cytometry In vitro

  • Yang Yu
  • Wei Ma
  • Lina Yu
  • Shuyuan Zhou
  • Guangshan Zhu
  • Wei Shi
Article
  • 3 Downloads

Abstract

In this paper, antibody-modified silica nanoparticles were successfully synthesized, and their average di-ameter was (109±9) nm. These particles were mixed with cell extracts to target a protein, then, an antibody labeled with fluorescein isothiocyanate(FITC) was added to form FITC-labeled nanoparticle complexes and the product was analyzed using flow cytometry. The results confirm that the intracellular proteins and biomarkers were precisely and sensitivity detected by the novel method in vitro. The FITC intensity of Akt antibody-conjugated particles was in-creased ≥10-fold compared with that of control samples in MCF-7 cells. Furthermore, it can also simultaneously measure several proteins when modified with different antibodies.

Keywords

Silica nanoparticle Flow cytometry Intracellular protein Biomarker 

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Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Yang Yu
    • 1
    • 2
  • Wei Ma
    • 3
  • Lina Yu
    • 4
  • Shuyuan Zhou
    • 5
  • Guangshan Zhu
    • 6
  • Wei Shi
    • 2
  1. 1.Department of Sports and HealthGuangzhou Institute of Physical EducationGuangzhouP. R. China
  2. 2.Key Laboratory for Molecular Enzymology and Engineering, Ministry of Education, College of Life ScienceJilin UniversityChangchunP. R. China
  3. 3.China-Japan Union HospitalJilin UniversityChangchunP. R. China
  4. 4.Key Laboratory of Oral Medicine, Guangzhou Institute of Oral DiseaseStomatology Hospital of Guangzhou Medical UniversityGuangzhouP. R. China
  5. 5.State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of ChemistryJilin UniversityChangchunP. R. China
  6. 6.Key Laboratory of Polyoxometalate Science, Ministry of Education, Faculty of ChemistryNortheast Normal UniversityChangchunP. R. China

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