Chemical Research in Chinese Universities

, Volume 33, Issue 5, pp 721–730 | Cite as

Analysis and identification of chemical constituents of fenugreek by UPLC-IT-MS n and UPLC-Q-TOF-MS

  • Jiaqi Wang
  • Wenyue Jiang
  • Zhongying Liu
  • Junhua Wang
  • Tianyue Fu
  • Yongsheng Wang


Fenugreek, a traditional Chinese medical plant, has been widely used as food ingredients because of its outstanding medicinal qualities. The hypoglycemic activity of fenugreek is one of its important pharmacological properties. Both of saponin and flavonoid components have the hypoglycemic activity and their contents in fenugreek are 4%—8% and 1%—2%, respectively. This paper focused on these two types of components to carry out purification research and chemical analysis. The heating reflux extraction method and macroporous resin purification method were designed to prepare the saponins and the flavonoids components from defatted fenugreek seeds. Petroleum ether ultrasonic skim and 70% ethanol refluxing were used for the extraction of saponins and flavonoid from fenugreek. The column of DM130 macroporous resin and D101 macroporous resin were respectively used to prepare fenugreek saponin and flavonoid components, and the total contents of them were 78.56% and 62.28%, respectively. The saponin and flavonoids were subsequently analyzed by an ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) along with an ultra-performance liquid chromatography and ion-trap tandem mass spectrometry(UPLC-IT-MS n ). As a result, 57 saponins and 19 flavonoid compounds were characterized. The obtained results will provide a theory basis for further research on fenugreek, as well as research and development of hypoglycemic new drugs.


Fenugreek Flavonoid Saponin Ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS) Ultra-performance liquid chromatography ion-trap tandem mass spectrometry(UPLC-IT-MSn


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  1. [1]
    Haghani K., Bakhtiyari S., Doost M. J., Can. J. Diabetes, 2016, 40, 135CrossRefGoogle Scholar
  2. [2]
    Sharma M. S., Choudhary P. R., J. Diet. Suppl., 2016, 14, 1CrossRefGoogle Scholar
  3. [3]
    Acharya S. N., Acharya K., Paul S., Basu S. K., Can. J. Plant Sci., 2017, 91, 99CrossRefGoogle Scholar
  4. [4]
    Rahmati-Yamchi M., Ghareghomi S., Haddadchi G., Milani M., Aghazadeh M., Daroushnejad H., Mol. Biol. Rep., 2014, 41, 6247CrossRefGoogle Scholar
  5. [5]
    Sharma N., Suresh S., Debnath A., Jha S., Front Biosci., 2017, 9, 246CrossRefGoogle Scholar
  6. [6]
    Prema A., Thenmozhi A. J., Manivasagam T., Essa M. M., Akbar M. D., Akbar M., PLoS One, 2016, 11, e0165955CrossRefGoogle Scholar
  7. [7]
    Pang X., Kang L., Yu H., Zhao Y., Xiong C., Zhang J., Shan J., Ma B., J. Sep. Sci., 2012, 35, 1538CrossRefGoogle Scholar
  8. [8]
    Kang L. P., Zhao Y., Pang X., Yu H. S., Xiong C. Q., Zhang J., Gao Y., Yu K., Liu C., Ma B. P., J. Pharm. Biomed. Anal., 2013, 74, 257CrossRefGoogle Scholar
  9. [9]
    Yoshikawa M., Murakami T., Komatsu H., Murakami N., Yamaha-ra J., Matsuda H., Chem. Pharm. Bull., 1997, 45, 81CrossRefGoogle Scholar
  10. [10]
    Yoshikawa M., Murakami T., Komatsu H., Yamahara J., Matsuda H., Heterocycles, 1998, 47, 397CrossRefGoogle Scholar
  11. [11]
    Murakami T., Kishi A., Matsuda H., Yoshikawa M., Chem. Pharm. Bull., 2000, 48, 994CrossRefGoogle Scholar
  12. [12]
    Xu X. M., Wang J., Yang H., Huang W. P., Yuan C. J., Chin. Tradit. Herbal. Drugs, 2005, 6, 1Google Scholar
  13. [13]
    Benayad Z., Gómez-Cordovés C., Es-Safi N. E., Int. J. Mol. Sci., 2014, 15, 20668CrossRefGoogle Scholar
  14. [14]
    Benayad Z., Gómez-Cordovés C., Es-Safi N. E., J. Food. Compos. Anal., 2014, 35, 21CrossRefGoogle Scholar
  15. [15]
    Król-Kogus B., Głód D., Krauze-Baranowska M., Matławska I., J. Chromatogr. A, 2014, 1367, 48CrossRefGoogle Scholar
  16. [16]
    Rayyan S., Fossen T., Andersen M., J. Agric. Food Chem., 2010, 58, 7211CrossRefGoogle Scholar
  17. [17]
    Hamden K., Jaouadi B., Salami T., Carreau S., Bejar S., Elfeki A., Biotechnol. Bioproc. E, 2010, 15, 745CrossRefGoogle Scholar
  18. [18]
    Gad M. Z., El-Sawalhi M. M., Ismail M. F., El-Tanbouly N. D., Mol. Cell Biochem., 2006, 281, 173CrossRefGoogle Scholar
  19. [19]
    Al-Habori M., Raman A., Lawrence M. J., Skett P., Int. J. Exp. Diabetes. Res., 2001, 2, 91CrossRefGoogle Scholar
  20. [20]
    Shan J. J., Ren J. W., Wu C. M., Zhao Y. M., J. Chin. Phar. Sci., 2008, 43, 1457Google Scholar
  21. [21]
    Wang H. Q., Guo S., Qian D. W., Qian Y. F., Duan J. A., J. Pharm. Biomed. Anal., 2012, 67/68, 16CrossRefGoogle Scholar
  22. [22]
    Tan G., Zhu Z., Jing J., Lv L., Lou Z., Zhang G. Y., Chai. Biomed. Chromatogr., 2011, 25, 913CrossRefGoogle Scholar
  23. [23]
    Dartora N., de Souza L. M., Santana A. P., Iacomini M., Valduga A. T., Gorin, P. A. J., Sassaki G. L., Food Chem., 2011, 129, 1453CrossRefGoogle Scholar
  24. [24]
    Guillarme D., Schappler J., Rudaz S., Veuthey J. L., Trends. Anal. Chem., 2010, 29, 15CrossRefGoogle Scholar
  25. [25]
    Theodoridis G. A., Gika H. G., Want E. J., Wilson I. D., Anal. Chim. Acta, 2012, 711, 7CrossRefGoogle Scholar

Copyright information

© Jilin University, The Editorial Department of Chemical Research in Chinese Universities and Springer-Verlag GmbH Germany 2017

Authors and Affiliations

  • Jiaqi Wang
    • 1
  • Wenyue Jiang
    • 1
    • 2
  • Zhongying Liu
    • 1
  • Junhua Wang
    • 1
  • Tianyue Fu
    • 1
  • Yongsheng Wang
    • 1
  1. 1.School of Pharmaceutical SciencesJilin UniversityChangchunP. R. China
  2. 2.Xiuzheng Pharmaceutical Group Company LimitedTonghuaP. R. China

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