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Label-free detection of biotin using nanoporous TiO2/DNA thin-film coated wavelength interrogated surface plasmon resonance sensors

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Abstract

The sensing sensitivity of the wavelength interrogated surface plasmon resonance(WISPR) biosensor could be improved by self-assembly of nanoporous thin-film of TiO2 nanoparticles/DNA(TiO2/DNA) n (n is the number of bilayer) on wavelength interrogated surface plasmon resonance(WISPR) chips. The growth behavior and surface structure of the nanoporous thin-film were investigated by UV-Vis spectroscopy and scanning electron microscopy, respectively. The home-made WISPR sensor with Krestchmann configuration consisted of a tungsten-halogen lamp as a photon source and a CCD camera as the detector. After the deposition of (DNA/TiO2) n thin film on WISPR chips, the resonance peak of the reflection spectra appeared in air. With the increases of n, the resonance wavelength gradually red shifted, which is consistent with the simulated results. After the optimization of the porous film, the WISPR biosensor was utilized to detect low-molecular-weight analytes, such as biotin. The result demonstrates that the sensitivity of [poly(styrene sulfonate)/polyally lamine hydrochlorides]5(PSS/PAH)5 could be 4 times higher than that of polyelectrolyte multilayer modified WISPR sensor.

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Authors and Affiliations

  1. Key Laboratory of Biosensor of Shandong Province, Biology Institute of Shandong Academy of Sciences, Jinan, 250014, P. R. China

    Qiushun Li, Hui Zheng & Jianguo Shi

  2. Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, 215163, P. R. China

    Hao He & Wenfei Dong

  3. The Second Hospital of Jilin University, Changchun, 130041, P. R. China

    Junnan Wang

  4. State Key Laboratory of Modern Optical Instrumentation, Zhejiang University, Hangzhou, 310027, P. R. China

    Mingyu Li

  5. State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing, 100190, P. R. China

    Zhimei Qi

Authors
  1. Qiushun Li
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  2. Hao He
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  3. Junnan Wang
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  4. Hui Zheng
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  5. Jianguo Shi
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  6. Mingyu Li
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  7. Wenfei Dong
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  8. Zhimei Qi
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Corresponding author

Correspondence to Junnan Wang.

Additional information

Supported by the National Natural Science Foundation of China(Nos.91123029, 61077066, 61340032), the National High Technology Research and Development Program of China(No.2012AA063302) and the Natural Science Foundation of Shandong Province, China(No.ZR2012CM029).

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Li, Q., He, H., Wang, J. et al. Label-free detection of biotin using nanoporous TiO2/DNA thin-film coated wavelength interrogated surface plasmon resonance sensors. Chem. Res. Chin. Univ. 30, 157–162 (2014). https://doi.org/10.1007/s40242-014-3312-y

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  • DOI: https://doi.org/10.1007/s40242-014-3312-y

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