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High-efficient isolation of plant viral RNA via TMAOH-modified Fe3O4 magnetic nanoparticles

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Chemical Research in Chinese Universities Aims and scope

Abstract

Magnetic nanoparticles show great potential in RNA enrichment and separation for rapid detection of viral infection. Fundamental studies on the interaction between RNA and nanoparticles with uniform size and surface property are necessary for designing better adsorbent and optimizing the conditions. In this study, monodispersed superparamagnetic magnetite(Fe3O4) nanoparticles were synthesized by thermal decomposition and modified with tetramethylammonium hydroxide[N(CH3)4OH, TMAOH] that become highly dispersible and stable in water. High-efficiency plant viral RNA adsorption onto TMAOH/Fe3O4 nanoparticles in the extracted solution of plant leaves was demonstrated. The changes of surface charge of TMAOH on the Fe3O4 nanoparticles with pH contribute to the RNA adsorption and elution. Separating viral RNA with magnetic nanoparticles could be a simple, quick and highly efficient method.

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References

  1. Hao R., Xing R. J., Xu Z. C., Hou Y. L., Gao S., Sun S. H., Adv. Mater., 2010, 22, 2729

    Article  CAS  Google Scholar 

  2. Qu J. M., Liu G., Wang Y. M., Hong R. Y., Adv. Powder Technol., 2012, 21, 461

    Article  Google Scholar 

  3. Jun Y. W., Huh Y. M., Choi J. S., Lee J. H., Song H. T., Kim S., Yoon S., Kim K. S., Shin J. S., Suh J. S., Cheon J., J. Am. Chem. Soc., 2005, 127, 5732

    Article  CAS  Google Scholar 

  4. Pan Y., Du X. W., Zhao F., Xu B., Chem. Soc. Rev., 2012, 41, 2912

    Article  CAS  Google Scholar 

  5. Wang L., Yang Z. M., Gao J. H., Xu K. M., Gu H. W., Zhang B., Zhang X. X., Xu B., J. Am. Chem. Soc., 2006, 128, 13358

    Article  CAS  Google Scholar 

  6. Jin Y. J., Liu F., Tong M. P., Hou Y. L., J. Hazard. Mater., 2012, 227/228, 461

    Article  Google Scholar 

  7. Cha D. M., Han Z. H., Ma T., Li B. H., Liu G. Q., Zhu W., Chem. J. Chinese Universities, 2013, 34(4), 760

    CAS  Google Scholar 

  8. Jiang H. H., Han X. Y., Li Z. L., Chen X. C., Hou Y. H., Gai L. G., Li D. C., Lu X. R., Fu T. L., Colloids and Surface A: Physicochem. Eng. Aspects, 2012, 401, 74

    Article  CAS  Google Scholar 

  9. Chiang C. L., Sung C. S., Chen C. Y., J. Magn. Magn. Mater., 2006, 305, 483

    Article  CAS  Google Scholar 

  10. Saiyed Z. M., Bochiwal C., Gorasia H., Telang S. D., Ramchand C. N., Anal. Biochem., 2006, 356, 306

    Article  CAS  Google Scholar 

  11. Ang D., Nguyen Q. V., Kayal S., Preiser P. R., Rawat R. S., Ramanujan R. V., Acta. Biomater., 2011, 7, 1319

    Article  CAS  Google Scholar 

  12. Kang Y. S., Risbud S., Rabolt J. F., Stroeve P., Chem. Mater., 1996, 8, 2209

    Article  CAS  Google Scholar 

  13. Jolivet J. P., Chaneac C., Tronc E., Chem. Comm., 2004, 481

    Google Scholar 

  14. Sun S. H., Zeng H., J. Am. Chem. Soc., 2002, 124, 8204

    Article  CAS  Google Scholar 

  15. Sun S. H., Zeng H., Robinson D. B., Raoux S., Rice P. M., Wang S. X., Li G. X., J. Am. Chem. Soc., 2004, 124, 273

    Article  Google Scholar 

  16. Park K. J., An Y. S., Hwang Y. S., Park J. G., Noh H. J., Kim J. Y., Park J. H., Hwang N. M., Hyeon T., Nat. Mater., 2004, 3, 891

    Article  CAS  Google Scholar 

  17. Park J., Lee E., Hwang N. M., Kang M. S., Kim S. C., Hwamg Y., Park J. G., Noh H. J., Kini J. Y., Park J. H., Hyeon T., Angew. Chem. Int. Edit., 2005, 44, 2872

    Article  CAS  Google Scholar 

  18. Pinna N., Grancharov S., Beato P., Antonietti M., Niederberger M., Chem. Mater., 2005, 17, 3044

    Article  CAS  Google Scholar 

  19. Liu B. J., Li X. Y., Chen W., Liu R. X., Ge G. L., Acta Phys.-Chim. Sin., 2010, 26, 784

    CAS  Google Scholar 

  20. Li X. Y., Tang Y., Ge G. L., Sci. China Phys. Mech. Astron., 2011, 54, 1766

    Article  CAS  Google Scholar 

  21. Taniguchi T., Nakagawa K., Watanabe T., Matsushita N., Yoshimura M., J. Phys. Chem. C, 2009, 113, 839

    Article  CAS  Google Scholar 

  22. Zhou S. X., Garnweitner G., Niederberger M., Antonietti M., Langmuir, 2007, 23, 9178

    Article  CAS  Google Scholar 

  23. Chen J., Li M. F., Plant Quarantine, 2007, 2, 94

    Google Scholar 

  24. Taylor J. I., Hurst C. D., Davies M. J., Sachsinger N., Bruce L. J., J. Chromatogr. A, 2000, 890, 159

    Article  CAS  Google Scholar 

  25. Daou T. J., Begin-Colin S., Grenèche J. M., Thomas F., Derory A., Bernhardt P., Legare P., Pourroy G., Chem. Mater., 2007, 19, 4494

    Article  CAS  Google Scholar 

  26. Sahoo Y., Pizem H., Fried T., Golodnitsky D., Burstein L., Sukenik C. N., Markovich G., Langmuir, 2001, 17, 7907

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory of Measurement and Standardization for Nanotechnology of Chinese Academy of Sciences, National Center for Nanoscience and Technology, Beijing, 100190, P. R. China

    Yan Tang, Linya Chu, Ning Sun, Renxiao Liu & Guanglu Ge

  2. Beijing Entry-exit Inspection and Quarantine Bureau, Beijing, 100026, P. R. China

    Xiaoli Zhao, Ning Sun & Congliang Deng

  3. University of Chinese Academy of Sciences, Beijing, 100049, P. R. China

    Yan Tang

  4. School of Materials Science and Engineering, Shandong University, Jinan, 250100, P. R. China

    Linya Chu

Authors
  1. Yan Tang
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  2. Xiaoli Zhao
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  3. Linya Chu
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  4. Ning Sun
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  5. Renxiao Liu
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  6. Congliang Deng
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  7. Guanglu Ge
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Corresponding authors

Correspondence to Congliang Deng or Guanglu Ge.

Additional information

Supported by the National Basic Research and Development Program of China(No.2011CB932803) and the Special Fund of the General Administration of Quality Supervision, Inspection and Quarantine of the People’s Republic of China(No.201110035).

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Tang, Y., Zhao, X., Chu, L. et al. High-efficient isolation of plant viral RNA via TMAOH-modified Fe3O4 magnetic nanoparticles. Chem. Res. Chin. Univ. 30, 23–26 (2014). https://doi.org/10.1007/s40242-014-3269-x

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  • DOI: https://doi.org/10.1007/s40242-014-3269-x

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