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Simultaneous determination of catecholamines and related metabolites by capillary electrophoresis with amperometric detection

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Abstract

An electrophoretic method was developed for the determination of several important catecholamine markers, namely norepinephrine, epinephrine, dopamine, metanephrine, vanillylmandelic acid and homovanillic acid in urine samples. Under the optimum conditions, the six marker compounds could be well separated with the major coexisting interference compound uric acid within 24 min at a separation voltage of 16 kV in a borate running buffer(80 mmol/L, pH=9.48). Highly linear response can be obtained over three orders of magnitude for the above markers with the low limits of detection ranging from 1.0 ng/mL to 5.0 ng/mL(S/N=3). The proposed capillary electrophoresis with amperometric detection(CE-AD) method has been used to simultaneously determine the six catecholamine markers in urine samples of healthy volunteers and patients suffering from different diseases avoiding redundant measurements and high assay cost; and the electrochemical profiles can suggest more diagnostic information of multiple diseases, which provides a promising and convenient entry into primary diagnoses of several clinical diseases.

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Authors and Affiliations

  1. Department of Chemistry, East China Normal University, Shanghai, 200241, P. R. China

    Hai-tao Zhang, Zhuo Li, Jun-bo Zhang, Yang Zhang, Jian-nong Ye & Qing-cui Chu

  2. Key Laboratory of Adolescent Health Assessment and Exercise Intervention, Ministry of Education, School of Sports and Health Care, East China Normal University, Shanghai, 200062, P. R. China

    Mei-jiang Zhang

Authors
  1. Hai-tao Zhang
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  2. Zhuo Li
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  3. Jun-bo Zhang
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  4. Yang Zhang
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  5. Jian-nong Ye
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  6. Qing-cui Chu
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  7. Mei-jiang Zhang
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Corresponding authors

Correspondence to Qing-cui Chu or Mei-jiang Zhang.

Additional information

Supported by the National Natural Science Foundation of China(No.21205042) and the Special Funds for the Development of Major Scientific Instruments and Equipment, China(No.2011YQ15007205).

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Zhang, Ht., Li, Z., Zhang, Jb. et al. Simultaneous determination of catecholamines and related metabolites by capillary electrophoresis with amperometric detection. Chem. Res. Chin. Univ. 29, 850–853 (2013). https://doi.org/10.1007/s40242-013-3132-5

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  • DOI: https://doi.org/10.1007/s40242-013-3132-5

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