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Gold nanoparticles based colorimetric detection of target DNA after loop-mediated isothermal amplification

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Chemical Research in Chinese Universities Aims and scope

Abstract

We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA. It is based on loop-mediated isothermal amplification(LAMP). Plain gold nanoparticles(AuNPs) are used to indicate the occurrence of LAMP. The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides( dNTPs) bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.

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Authors and Affiliations

  1. Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou, 310058, P. R. China

    Chao Zhou, Ying Mu, Meng-chao Yang, Ying Zhang, Wei Jin, Qi Song, Zhong-yu Wu & Qin-han Jin

  2. Central Laboratory, Affiliated Hospital of Guiyang Medical College, Guiyang, 550004, P. R. China

    Qing-qing Wu

  3. School of Mathematics and Science, Shijiazhuang University of Economics, Shijiazhuang, 050031, P. R. China

    Wei Xu

Authors
  1. Chao Zhou
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  2. Ying Mu
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  3. Meng-chao Yang
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  4. Qing-qing Wu
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  5. Wei Xu
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  6. Ying Zhang
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  7. Wei Jin
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  8. Qi Song
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  9. Zhong-yu Wu
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  10. Qin-han Jin
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Corresponding authors

Correspondence to Ying Mu or Qin-han Jin.

Additional information

Supported by the National Natural Science Foundation of China(Nos.31070772, 31270907), the Doctoral Program of Higher Education of China(No.20090101110136), the Science and Technology Programs of Zhejiang Province of China(No.2011C37029) and the Fund of State Key Laboratory of Industrial Control Technology of China.

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Zhou, C., Mu, Y., Yang, Mc. et al. Gold nanoparticles based colorimetric detection of target DNA after loop-mediated isothermal amplification. Chem. Res. Chin. Univ. 29, 424–428 (2013). https://doi.org/10.1007/s40242-013-2371-9

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  • DOI: https://doi.org/10.1007/s40242-013-2371-9

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