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Identification of porcine reproductive and respiratory syndrome virus inhibitors through an oriented screening on natural products

Chemical Research in Chinese Universities volume 29, pages290–293(2013)Cite this article

Abstract

Porcine reproductive and respiratory syndrome(PRRS) caused by porcine reproductive and respiratory syndrome virus(PRRSV) is one of the most infectious diseases in the swine industry worldwide, causing big economic losses. Vaccines are major weapons against PRRSV, however, current available vaccines have several limitations. Developing chemical drugs as alternatives is required. On the basis of traditional medical knowledge, we purposely selected 15 natural products originated from Chinese herbs with anti-infectious effects. Their antiviral activities were evaluated by PRRSV-induced cytopathic effect(CPE) on MARC-145 cells and reverse transcription polymerase chain reaction(RT-PCR) assay. Compounds ethoxysanguinarine(EOSG) and atractylodinol were found to be the hits which could significantly reduce PRRSV-associated CPE with 50% inhibited concentration(IC50) values of 7.9 and 39.4 μmol/L, respectively. Meanwhile, compounds ethoxysanguinarine and atractylodinol significantly decreased mRNA expression of ORF7 gene in a dose-dependent manner. Study results suggest that compounds ethoxysanguinarine and atractylodinol may be useful anti-PRRSV drugs for swine industry or the hits for further lead optimization.

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Affiliations

  1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming, 650201, P. R. China
    • Wen-gui Li
    • , Fei-yan Dai
    • , Ge-fen Yin
    •  & Jun-long Bi
  2. State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, 650204, P. R. China
    • Yong-xian Cheng
  3. Guangxi Key Laboratory of Functional Phytochemicals Research and Utilization, Guangxi Institute of Botany, Guilin, 541006, P. R. China
    • Dian-peng Li
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  1. Wen-gui Li
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Corresponding author

Correspondence to Yong-xian Cheng.

Additional information

Supported by the Project of Talent Scholarship for the Youth of Yunnan Province of China(No.2007PY01-48), the Sino-German International Collaboration Project from Yunnan Province of China(No.2009AC011), the Project of Natural Compound Library Construction from Chinese Academy of Sciences(No.KSCX2-EW-R-15) and the Yunnan Provincial Program for Excellent Scientists, China(No.2009CI125).

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Li, W., Dai, F., Cheng, Y. et al. Identification of porcine reproductive and respiratory syndrome virus inhibitors through an oriented screening on natural products. Chem. Res. Chin. Univ. 29, 290–293 (2013). https://doi.org/10.1007/s40242-013-2300-y

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Keywords

  • Porcine reproductive and respiratory syndrome virus(PRRSV)
  • Natural product
  • Cytopathic effect
  • Antiviral activity