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Immobilization of hemoglobin on cobalt nanoparticles-modified indium tin oxide electrode: Direct electrochemistry and electrocatalytic activity

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Abstract

A cobalt nanoparticles-attached indium tin oxide(CoNPs/ITO) electrode was applied to the immobilization of hemoglobin(Hb) and an Hb modified CoNPs/ITO electrode(Hb/CoNPs/ITO) was prepared. The direct electron transfer of Hb was shown by the well-behaved voltammetric responses for Hb/CoNPs/ITO electrode and the effects of scan rate and pH value were observed. Based on the catalytic activity of Hb immobilized on the CoNPs/ITO electrode toward the reduction of H2O2, a mediator-free H2O2 sensor was developed. A linear relationship existed between the catalytic current and the H2O2 concentration in a range of 1.0–100.0 μmol/L with a detection limit (S/N=3) of 0.2 μmol/L.

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Authors and Affiliations

  1. College of Materials Science and Engineering, Shandong University of Science and Technology, Qingdao, 266590, P. R. China

    Shuo-qi Li

  2. College of Chemistry, Beijing Normal University, Beijing, 100875, P. R. China

    Jing-bo Hu

Authors
  1. Shuo-qi Li
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  2. Jing-bo Hu
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Correspondence to Shuo-qi Li.

Additional information

Supported by the National Natural Science Foundation of China(No.20211130505) and the Fundamental Research Funds for the Central Universities of China.

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Li, Sq., Hu, Jb. Immobilization of hemoglobin on cobalt nanoparticles-modified indium tin oxide electrode: Direct electrochemistry and electrocatalytic activity. Chem. Res. Chin. Univ. 29, 563–567 (2013). https://doi.org/10.1007/s40242-013-2273-x

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  • DOI: https://doi.org/10.1007/s40242-013-2273-x

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