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Improvement in mechanical properties and biodegradability of PLA using poly(ethylene glycol) and triacetin for antibacterial wound dressing applications

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Abstract

Wound is among the most common injuries. A suitable wound dressing has a significant effect on the healing process. In this study, a porous wound dressing was prepared using poly (lactic acid) (PLA) and two plasticizers, polyethylene glycol (PEG) and triacetin (TA), through solvent casting method. For antibacterial activities, metronidazole was incorporated in the structure. The morphology was investigated by scanning electron microscopy (SEM). In addition, the effect of plasticizers ratio on porosity growth was evaluated. It was also observed that each had a unique effect on the structure’s porosity. The mechanical properties confirmed the effect of both plasticizers on increasing polymer softness and flexibility, and the most similar formulations to human skin in terms of mechanical properties were introduced. According to the results, TA had stronger effect on mechanical properties. The differential scanning calorimetry (DSC) showed the effect of increasing plasticizer concentration on crystalline structure and Tm reduction of PLA. The water contact angle measurement showed that both plasticizers enhanced hydrophilic characteristics of PLA, and this effect was weaker in PEG-containing formulations. The in vitro degradation study showed biodegradability, as a desirable property in wound dressing. Results suggested that higher degradation can be obtained by both plasticizers at the same time. The results also showed that PEG was more effective in enhancing water absorbency. In vitro drug release study indicated an explosive release and the highest amount was 85% over 186 h. The antibacterial activity test confirmed the effectiveness of the drug in preventing bacterial growth in the drug-containing formulations, while it showed the antibacterial property of TA. MTT assay was performed and the cellular toxicity of the formulations was checked and those that revealed the least toxicity were introduced.

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Funding

The author(s) received no financial support for the research, authorship, and/or publication of this article.

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Authors and Affiliations

  1. Faculty of Interdisciplinary Science and Technology, Tarbiat Modares University, Tehran, Iran

    Bita Darabian & Hamed Bagheri

  2. Pharmaceutical Science Research Center, Health Institute, Kermanshah University of Medical Science, Kermanshah, Iran

    Soheila Mohammadi

Authors
  1. Bita Darabian
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  2. Hamed Bagheri
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  3. Soheila Mohammadi
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Contributions

HB: designed and performed experiments; BD: performed experiments and co-wrote the paper: SM: co-wrote the paper.

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Correspondence to Hamed Bagheri.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

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Darabian, B., Bagheri, H. & Mohammadi, S. Improvement in mechanical properties and biodegradability of PLA using poly(ethylene glycol) and triacetin for antibacterial wound dressing applications. Prog Biomater 9, 45–64 (2020). https://doi.org/10.1007/s40204-020-00131-6

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  • DOI: https://doi.org/10.1007/s40204-020-00131-6

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