Abstract
Analyzing municipal wastewater for the presence of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) helps to evaluate the efficacy of treatment systems in mitigating virus-related health risks. This research investigates wastewater treatment plants’ (WWTPs) performance in the reduction of SARS-CoV-2 from municipal wastewater in Tehran, Iran. SARS-CoV-2 RNA was measured within sewers, at the inlets, and after the primary and secondary treatment stages of three main WWTPs. Within sewers, the average virus titer stood at 58,600 gc/L, while at WWTP inlets, it measured 38,136 gc/L. A substantial 67% reduction in virus titer was observed at the inlets, accompanied by a 2-log reduction post-primary treatment. Remarkably, the biological treatment process resulted in complete virus elimination across all plants. Additionally, a notable positive correlation (r > 0.8) was observed between temperature and virus titer in wastewater. Using wastewater-based epidemiology (WBE) technique and the estimated SARS-CoV-2 RNA shedding rates, the infection prevalence among populations served by WWTPs found to be between 0.128% to 0.577%. In conclusion, this research not only advances our understanding of SARS-CoV-2 dynamics within wastewater treatment systems but also provides practical insights for enhancing treatment efficiency and implementing the feasibility of WBE strategies in Tehran. These implications contribute to the broader efforts to protect public health and mitigate the impact of future viral outbreaks.
Graphical abstract
Similar content being viewed by others
Data availability
Data sets generated during the current study are available from the corresponding author upon request.
References
La Rosa G, Bonadonna L, Lucentini L, Kenmoe S, Suffredini E. Coronavirus in water environments: occurrence, persistence and concentration methods-a scoping review. Water Res. 2020;179: 115899.
Foladori P, Cutrupi F, Segata N, Manara S, Pinto F, Malpei F, et al. SARS-CoV-2 from faeces to wastewater treatment: what do we know? a review. Sci Total Environ. 2020;743: 140444.
Corpuz MVA, Buonerba A, Vigliotta G, Zarra T, Ballesteros F Jr, Campiglia P, et al. Viruses in wastewater: occurrence, abundance and detection methods. Sci Total Environ. 2020;745: 140910.
Mandal P, Gupta AK, Dubey BK. A review on presence, survival, disinfection/removal methods of coronavirus in wastewater and progress of wastewater-based epidemiology. J Environ Chem Eng. 2020;8(5):104317.
Ahmed W, Angel N, Edson J, Bibby K, Bivins A, O’Brien JW, et al. First confirmed detection of SARS-CoV-2 in untreated wastewater in Australia: a proof of concept for the wastewater surveillance of COVID-19 in the community. Sci Total Environ. 2020;728:138764.
La Rosa G, Iaconelli M, Mancini P, Bonanno Ferraro G, Veneri C, Bonadonna L, et al. First detection of SARS-CoV-2 in untreated wastewaters in Italy. Sci Total Environ. 2020;736:139652.
Carducci A, Federigi I, Liu D, Thompson JR, Verani M. Making waves: coronavirus detection, presence and persistence in the water environment: state of the art and knowledge needs for public health. Water Res. 2020;179: 115907.
Ahmed W, Tscharke B, Bertsch PM, Bibby K, Bivins A, Choi P, et al. SARS-CoV-2 RNA monitoring in wastewater as a potential early warning system for COVID-19 transmission in the community: a temporal case study. Sci Total Environ. 2021;761:144216.
Medema G, Heijnen L, Elsinga G, Italiaander R, Brouwer A. Presence of SARS-coronavirus-2 RNA in sewage and correlation with reported COVID-19 prevalence in the early stage of the epidemic in the Netherlands. Environ Sci Pollut Res. 2020;7(7):511–6.
Gholipour S, Mohammadi F, Nikaeen M, Shamsizadeh Z, Khazeni A, Sahbaei Z, et al. COVID-19 infection risk from exposure to aerosols of wastewater treatment plants. Chemosphere. 2021;273: 129701.
Qamsari EM, Mohammadi P. Evaluation of SARS-CoV-2 RNA presence in treated and untreated hospital sewage. Water Air Soil Pollut. 2023;234(4):273.
Zaneti RN, Girardi V, Spilki FR, Mena K, Westphalen APC, da Costa Colares ER, et al. Quantitative microbial risk assessment of SARS-CoV-2 for workers in wastewater treatment plants. Sci Total Environ. 2021;754: 142163.
Rimoldi SG, Stefani F, Gigantiello A, Polesello S, Comandatore F, Mileto D, et al. Presence and vitality of SARS-CoV-2 virus in wastewaters and rivers. MedRxiv. 2020:2020.05.01.20086009.
Randazzo W, Truchado P, Cuevas-Ferrando E, Simón P, Allende A, Sánchez G. SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area. Water Res. 2020;181: 115942.
Wang J, Feng H, Zhang S, Ni Z, Ni L, Chen Y, et al. SARS-CoV-2 RNA detection of hospital isolation wards hygiene monitoring during the coronavirus disease 2019 outbreak in a Chinese hospital. Int J Infect Dis. 2020;94:103–6.
Kumar M, Patel AK, Shah AV, Raval J, Rajpara N, Joshi M, et al. First proof of the capability of wastewater surveillance for COVID-19 in India through detection of genetic material of SARS-CoV-2. Sci Total Environ. 2020;746: 141326.
Peccia J, Zulli A, Brackney DE, Grubaugh ND, Kaplan EH, Casanovas-Massana A, et al. SARS-CoV-2 RNA concentrations in primary municipal sewage sludge as a leading indicator of COVID-19 outbreak dynamics. medRxiv. 2020;20105999. https://doi.org/10.1101/2020.05.19.20105999.
Kocamemi BA, Kurt H, Sait A, Sarac F, Saatci AM, Pakdemirli B. SARS-CoV-2 detection in Istanbul wastewater treatment plant sludges. medRxiv. 2020;20099358. https://doi.org/10.1101/2020.05.12.20099358.
Westhaus S, Weber F-A, Schiwy S, Linnemann V, Brinkmann M, Widera M, et al. Detection of SARS-CoV-2 in raw and treated wastewater in Germany–suitability for COVID-19 surveillance and potential transmission risks. Sci Total Environ. 2021;751: 141750.
Arora S, Nag A, Sethi J, Rajvanshi J, Saxena S, Shrivastava SK, et al. Sewage surveillance for the presence of SARS-CoV-2 genome as a useful wastewater based epidemiology (WBE) tracking tool in India. Water Sci Technol. 2020;82(12):2823–36.
González-Reyes JR, Hernández-Flores MdlL, Paredes-Zarco JE, Téllez-Jurado A, Fayad-Meneses O, Carranza-Ramírez L. Detection of SARS-CoV-2 in Wastewater Northeast of Mexico City: Strategy for Monitoring and Prevalence of COVID-19. Int J Environ Res Public Health. 2021;18(16):8547.
WHO. Guidelines for environmental surveillance of poliovirus circulation. In: Global Polio Eradication Initiative. 2015. https://polioeradication.org/wp-content/uploads/2016/07/GPLN_GuidelinesES_April2015.pdf.
Lu D, Huang Z, Luo J, Zhang X, Sha S. Primary concentration – The critical step in implementing the wastewater based epidemiology for the COVID-19 pandemic: A mini-review. Sci Total Environ. 2020;747:141245.
R Core Team. R: A language and environment for statistical computing. R Foundation for Statistical Computing; Vienna, Austria; 2021. URL https://www.R-project.org/. Accessed Oct 2023.
Rose C, Parker A, Jefferson B, Cartmell E. The characterization of feces and urine: a review of the literature to inform advanced treatment technology. Crit Rev Environ Sci Technol. 2015;45(17):1827–79.
Wölfel R, Corman VM, Guggemos W, Seilmaier M, Zange S, Müller MA, et al. Virological assessment of hospitalized patients with COVID-2019. Nature. 2020;581(7809):465–9.
Barceló D. Wastewater-based epidemiology to monitor COVID-19 outbreak: present and future diagnostic methods to be in your radar. CSCEE. 2020;2:100042.
Steven Percival MVY, Williams DW, Chalmers RM, Gray NF. Free and combined chlorine. In: Microbiology of waterborne diseases : microbiological aspects and risks. second ed. London: Academic Press: Amsterdam ; Boston : Elsevier/Academic Press; 2014. pp. 571–90.
Deborde M, von Gunten U. Reactions of chlorine with inorganic and organic compounds during water treatment—Kinetics and mechanisms: a critical review. Water Res. 2008;42(1):13–51.
Gundy PM, Gerba CP, Pepper IL. Survival of coronaviruses in water and wastewater. Food Environ Virol. 2009;1(1):10–4.
watertechnologies.com. Chapter 27 - Chlorine And Chlorine Alternatives. In: Handbook of industrial water treatment. SUEZ Water Technologies; 2021. https://www.watertechnologies.com/handbook/chapter-27-chlorine-and-chlorine-alternatives.
Serra-Compte A, González S, Arnaldos M, Berlendis S, Courtois S, Loret JF, et al. Elimination of SARS-CoV-2 along wastewater and sludge treatment processes. Water Res. 2021;202: 117435.
Abu Ali H, Yaniv K, Bar-Zeev E, Chaudhury S, Shagan M, Lakkakula S, et al. Tracking SARS-CoV-2 RNA through the wastewater treatment process. ACS ES&T Water. 2021;1(5):1161–7.
Brisebois E, Veillette M, Dion-Dupont V, Lavoie J, Corbeil J, Culley A, et al. Human viral pathogens are pervasive in wastewater treatment center aerosols. Res J Environ Sci. 2018;67:45–53.
Wigginton KR, Pecson BM, Sigstam T, Bosshard F, Kohn T. Virus inactivation mechanisms: impact of disinfectants on virus function and structural integrity. Environ Sci Technol. 2012;46(21):12069–78.
Randazzo W, Truchado P, Cuevas-Ferrando E, Simón P, Allende A, Sánchez G. SARS-CoV-2 RNA in wastewater anticipated COVID-19 occurrence in a low prevalence area. Water Res. 2020;181: 115942.
Sherchan SP, Shahin S, Ward LM, Tandukar S, Aw TG, Schmitz B, et al. First detection of SARS-CoV-2 RNA in wastewater in North America: a study in Louisiana, USA. Sci Total Environ. 2020;743: 140621.
Haramoto E, Malla B, Thakali O, Kitajima M. First environmental surveillance for the presence of SARS-CoV-2 RNA in wastewater and river water in Japan. Sci Total Environ. 2020;737: 140405.
Nasseri S, Yavarian J, Baghani AN, Azad TM, Nejati A, Nabizadeh R, et al. The presence of SARS-CoV-2 in raw and treated wastewater in 3 cities of Iran: Tehran, Qom and Anzali during coronavirus disease 2019 (COVID-19) outbreak. J Environ Health Sci Eng. 2021;19:573–84.
Westhaus S, Weber F-A, Schiwy S, Linnemann V, Brinkmann M, Widera M, et al. Detection of SARS-CoV-2 in raw and treated wastewater in Germany–suitability for COVID-19 surveillance and potential transmission risks. Sci Total Environ. 2021;751: 141750.
Hasan SW, Ibrahim Y, Daou M, Kannout H, Jan N, Lopes A, et al. Detection and quantification of SARS-CoV-2 RNA in wastewater and treated effluents: Surveillance of COVID-19 epidemic in the United Arab Emirates. Sci Total Environ. 2021;764: 142929.
Pasalari H, Ataei-Pirkooh A, Gholami M, Azhar IR, Yan C, Kachooei A, et al. Is SARS-CoV-2 a concern in the largest wastewater treatment plant in middle east? Heliyon. 2023;9(6):e16607.
Rimoldi SG, Stefani F, Gigantiello A, Polesello S, Comandatore F, Mileto D, et al. Presence and infectivity of SARS-CoV-2 virus in wastewaters and rivers. Sci Total Environ. 2020;744: 140911.
Wang J, Shen J, Ye D, Yan X, Zhang Y, Yang W, et al. Disinfection technology of hospital wastes and wastewater: Suggestions for disinfection strategy during coronavirus Disease 2019 (COVID-19) pandemic in China. Environ Pollut. 2020;262: 114665.
Alahdal HM, Ameen F, AlYahya S, Sonbol H, Khan A, Alsofayan Y, et al. Municipal wastewater viral pollution in Saudi Arabia: effect of hot climate on COVID-19 disease spreading. Environ Sci Pollut Res. 2023;30(10):25050–7.
Dutta H, Kaushik G, Dutta V. Wastewater-based epidemiology: a new frontier for tracking environmental persistence and community transmission of COVID-19. Environ Sci Pollut Res. 2022;29(57):85688–99.
Yaniv K, Shagan M, Lewis YE, Kramarsky-Winter E, Weil M, Indenbaum V, et al. City-level SARS-CoV-2 sewage surveillance. Chemosphere. 2021;283: 131194.
Acknowledgements
Funding for this study (Grant No.: 48954–110-2–99) was provided by the Office of R&D and Industrial Relations of Water and Wastewater Engineering Company, Tehran, Iran.
Author information
Authors and Affiliations
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests or any personal relationships that could have appeared to influence the content of this paper.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Hadi, M., Kheiri, R., Baghban, M. et al. The occurrence of SARS-CoV-2 in Tehran’s municipal wastewater: performance of treatment systems and feasibility of wastewater-based epidemiology. J Environ Health Sci Engineer (2024). https://doi.org/10.1007/s40201-024-00897-y
Received:
Accepted:
Published:
DOI: https://doi.org/10.1007/s40201-024-00897-y