Abstract
Purpose
More than 20 genetically modified (GM) food crops including rice have been approved in many countries. GM rice and derived products have not yet been approved in India so they are considered as unauthorized genetically modified organisms (GMOs) in the country. Therefore it is important to track whether the rice containing food items, available in the marketplace are GMO-free.
Methods
A pilot study was conducted to check the GM status of 30 samples of packed rice grains and processed food products with rice as an ingredient, using polymerase chain reaction (PCR) assays targeting Cauliflower Mosaic Virus 35 S promoter (P-35 S), nopaline synthase terminator (T-nos), phosphinothricin-N-acetyltransferase (pat) and cry1Ac gene, which could cover screening for all the globally approved GM rice events.
Results
Based on the results, none of the samples tested were found positive for P-35 S, T-nos, pat and cry1Ac.
Conclusion
The unauthorized presence of GM rice ingredients was not detected in the samples tested. Such studies may further be conducted for the testing of GM ingredients derived from cereals other than rice in the food products imported from the country where GM events of respective cereal crop are approved, as a part of regulatory requirement.
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Data availability
Not applicable.
References
https://www.isaaa.org/ (last accessed on 05.11.2023).
Ye X, Al-Babili S, Klöti A, Zhang J, Lucca P, Beyer P, Potrykus I. Engineering the provitamin A (beta-carotene) biosynthetic pathway into (carotenoid-free) rice endosperm. Science. 2000;287(5451):303–5.
https://www.isaaa.org/gmapprovaldatabase/ (last accessed on 05.11.2023).
Randhawa GJ, Singh M, Grover M. Bioinformatic analysis for allergenicity assessment of Bacillus thuringiensis cry proteins expressed in insect-resistant food crops. Food Chem Toxicol. 2011;49(2):356–62.
https://geacindia.gov.in/Uploads/MoMPublished/2018-geac-134.pdf (last accessed on 01.12.2022).
EPA., 1986. The Environment (Protection) Act, 1986.
Rules. 1989. Rules for the manufacture, use/import/export and storage of hazardous microorganisms/ genetically engineered organisms or cells.
Singh M, Randhawa G. Transboundary movement of genetically modified organisms in India: current scenario and a decision support system. Food Cont. 2016;68:20–4.
https://www.fssai.gov.in/upload/uploadfiles/files/Draft_Notification_GM_Food_21_11_2022.pdf (last accessed on 05.11.2023).
Ramos-Gómez S, Busto MD, Perez-Mateos M, Ortega N. (2014) Development of a method to recovery and amplification DNA by real-time PCR from commercial vegetable oils. Food Chem. 2014; 158: 374– 83.
Singh M, Sodhi KK, Paliwal A, Sharma S, Randhawa G. (2021) Efficient DNA extraction procedures for processed food derivatives - a critical step to ensure quality for GMO analysis. Food Anal Meth. 2021; 14: 2249-61.
Mäde D, Degner C, Grohmann L. Detection of genetically modified rice: a construct-specific real-time PCR method based on DNA sequences from transgenic bt rice. Eur Food Res Technol. 2006;224(2):271–78.
Babekova R, Funk T, Pecoraro S, Engel KH, Busch U. Development of an event-specific real-time PCR detection method for the transgenic bt rice line KMD1. Eur Food Res Technol. 2009;228:707–16.
Randhawa GJ, Singh M. Multiplex, construct-specific, and real-time PCR-based analytical methods for Bt rice with cry1Ac gene. J AOAC Int. 2012;95(1):186–94.
Shin MK, Jeon SM, Koo YE. Development of a rapid detection method for genetically modified rice using the ultra-fast PCR system. Food Sci Biotechnol. 2022;31(2):175–82.
Elsanhoty RM, Al-Turki AI, Ramadan MF. Prevalence of genetically modified rice, maize, and soy in Saudi food products. Appl Biochem Biotechnol. 2013;171(4):883–99.
Safaei P, Aghaee EM, Khaniki GJ, Afshari S, Rezaie S. A simple and accurate PCR method for detection of genetically modified rice. J Env Health Sci Eng. 2019;17(2):847–51.
Avsar B, Sadeghi S, Turkec A, Lucas SJ. Identification and quantitation of genetically modified (GM) ingredients in maize, rice, soybean and wheat-containing retail foods and feeds in Turkey. J Food Sci Technol. 2020;57(2):787–93.
Taberlet P, Gielly L, Patou G, Bouvet J. 1991 Universal primers for amplification of three non-coding regions of chloroplast DNA. Plant Mol Biol. 1991; 17: 1105-9.
Singh M, Paliwal A, Kaur K, Palit P, Randhawa G. Development and utilization of analytical methods for rapid GM detection in processed food products: a case study for regulatory requirement. J Plant Biochem Biotechnol. 2023;32:511–24.
Lipp M, Brodmann P, Pietsch K, Pauwels J, Anklam E. IUAPC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder. J AOAC Int. 1999;82:923–28.
Permingeat HR, Reggiardo MI, Vallejos RH. Detection and quantification of transgenes in grains by multiplex and real-time PCR. J Agric Food Chem. 2002;50:4431–36.
Randhawa GJ, Singh M, Chhabra R, Sharma R. Qualitative and quantitative molecular testing methodologies and traceability systems for commercialised Bt cotton events and other Bt crops under field trials in India. Food Anal Meth. 2010;3:295–303.
Safaei P, Rezaie S, Alimohammadi M, Afshari SAK, Mehdizadeh M, Aghaee EM. Qualitative PCR-based detection of genetically modified soy and maize products in Iran, Int J Food Prop. 2020; 23:1: 459– 69.
Acknowledgements
The authors duly acknowledge the Indian Council of Agricultural Research (ICAR), New Delhi, Director, ICAR-National Bureau of Plant Genetic Resources, New Delhi, and Head, Division of Genomic Resources for providing necessary facilities. Grant provided by ICAR and Department of Biotechnology is duly acknowledged.
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ICAR and Department of Biotechnology.
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U.B. conducted studies for GMO testing of packed rice samples; P.G.: conducted studies for GMO testing of processed food products/ samples; K.K.: verification of GMO test results; M.S.: conceptualization and planning of the work, technical supervision, writing the original draft, formal analysis and interpretation of findings.
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Bharti, U., Gaur, P., Kaur, K. et al. Tracking genetically modified (GM) rice ingredients in samples of packed rice and food products from the marketplace in India: a pilot study for regulatory compliance. J Environ Health Sci Engineer (2024). https://doi.org/10.1007/s40201-024-00892-3
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DOI: https://doi.org/10.1007/s40201-024-00892-3