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Association of common single-nucleotide polymorphism of HHEX with type 2 diabetes mellitus

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Journal of Diabetes & Metabolic Disorders Aims and scope Submit manuscript

Abstract

Background

Type 2 diabetes mellitus (T2DM) is a complex metabolic disease that occurs as a result of insulin resistance and low insulin production. T2DM involves many organ systems that include macro-vascular and micro-vascular complications. Several genome-wide association studies (GWAS) and candidate gene studies have suggested a large number of single nucleotide polymorphisms (SNPs) on several genes such as HHEX that were associated with T2DM susceptibility. The current study aims to look at the relationship between the risk of T2DM and the HHEX gene variant rs7923837.

Methods

In this case-control study genotyping of rs7923837 of the HHEX gene was performed using the PCR-RFLP and Sanger sequencing method.

Results

Frequencies of GG genotype of rs7923837 polymorphism of HHEX among subjects with and without diabetes mellitus were 33.77% and 25.47% respectively. Corresponding prevalence for the AG genotype was 51.08% and 64.15% among subjects with and without diabetes mellitus respectively. The differences were not statistically significant (p = 0.08).

Conclusion

Our study revealed that polymorphisms rs7923837 of HHEX were not associated with T2DM.

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Data availability

This project has no associated data to be available.

Abbreviations

T2DM:

type 2 diabetes mellitus

GWAS:

genome-wide association studies

HHEX:

hematopoietically expressed homeobox

SNPs:

single nucleotide polymorphisms

FPG:

fasting plasma glucose

HbA1c:

hemoglobin A1c

OGTT:

oral glucose tolerance test

ADA:

American Diabetes Association

PCR:

polymerase chain reaction

IDF:

International Diabetes Federation

DR:

diabetic retinopathy

DN:

diabetic nephropathy

OR:

odds ratio

CI:

confidence interval

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Acknowledgements

The authors would like to express their gratitude to T2DM patients for their participation in this study.

Funding

There was no funding to report.

Author information

Authors and Affiliations

  1. Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran

    Saeedeh Hasti, Mandana Hasanzad & Farhad Adhami Moghadam

  2. Endocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mani Mirfeizi

  3. Department of Community Medicine, Zabol University of Medical Sciences, Zabol, Iran

    Mahdi Afshari

  4. Personalized Medicine Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Mandana Hasanzad & Hamid Reza Aghaei Meybodi

  5. Department of Internal Medicine, School of Medicine, Shariati Hospital, Tehran University of Medical Sciences, Tehran, Iran

    Hamid Reza Aghaei Meybodi

Authors
  1. Saeedeh Hasti
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  2. Mani Mirfeizi
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  3. Mahdi Afshari
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  4. Mandana Hasanzad
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  5. Farhad Adhami Moghadam
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  6. Hamid Reza Aghaei Meybodi
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Contributions

MH managed the project; SH drafted the manuscript and performed laboratory methods; MA performed the statistical analysis; MM, FAM, and HRAM: provided clinical guidance to the research. All authors contributed to and approved the final version of the manuscript.

Corresponding authors

Correspondence to Farhad Adhami Moghadam or Hamid Reza Aghaei Meybodi.

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Consent for publication

All patients signed written consent forms and the study was approved by the ethical committee (Ethic Code: IR.TMU.REC.1395.19).

Competing interests

There are no conflicts of interest declared by the authors.

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Hasti, S., Mirfeizi, M., Afshari, M. et al. Association of common single-nucleotide polymorphism of HHEX with type 2 diabetes mellitus. J Diabetes Metab Disord (2024). https://doi.org/10.1007/s40200-024-01407-5

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  • DOI: https://doi.org/10.1007/s40200-024-01407-5

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