Skip to main content

Advertisement

SpringerLink
Log in

In vivo antioxidant and hypoglycaemic potentials of Rivina humilis extract against streptozotocin induced diabetes and its complications in wistar rats

  • Research article
  • Published:
Journal of Diabetes & Metabolic Disorders Aims and scope Submit manuscript

Abstract

Purpose

This current research study was designed to investigate beneficial effects of R. humilis (Rivina humilis) against streptozotocin-induced diabetic rats.

Methods

The R. humilis ethanol extract was prepared using soxhlet and its phenol content was determined. The type-2 diabetes was induced in rats by giving fructose mixed drinking water and single dose of streptozotocin. Oral glucose tolerance test (OGTT) was performed after 72 h of streptozotocin to check ability of extract to utilize oral glucose load with 2 h. The extract was also tested for its potentials to reduce blood glucose (BGL) and diabetic complications by administering to diabetic rats for 21 days. Blood glucose was determined on day 1, 7, 14 and 21. At 21st day, blood samples were collected from experimental rats were euthanized to collect pancreas and liver. Liver and kidney function tests, HbAc1 and lipid profile was established from blood samples. Pancreas was subjected to histopathological examination and liver was used to determine antioxidant enzymes. In vitro study was done to investigate the effect of extract on glucose utilization by rat hemidiaphragm.

Results

In OGTT, administration of extract could stimulate glucose utilization which was witnessed by significant BGL reduction at 90 and 120 min in therapeutic groups compare to diabetics. In chronic study, we observed significant reduction in BGL on 21st day and all tests performed to determine liver and kidney function, HbAc1, vitamin E were normal in extract treated groups. There was significant increase in liver antioxidant enzymes in therapeutic groups which revealed regeneration of β-cells in therapeutic groups.

Conclusion

The results of research demonstrated significant antidiabetic potentials in R. humilis.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

Abbreviations

ALT:

Alanine transferase

AST:

aspartate transferase

CAP:

Catalase peroxidase

DB:

Diabetes mellitus

EERH:

Ethanol extract of Rivina humilis

GSH:

Glutathione reductase

GST:

Glutathione S transferase

SOD:

Superoxide dismutase

TC:

Total cholesterol

TG:

Triglycerides

References

  1. ChenYC, Tung YC, Liu SY, Lee CT, Tsai WY. Clinical characteristics of type 1 diabetes mellitus in taiwanese children aged younger than 6 years: a single-center experience. J Formos Med Assoc. 2017;116(5):340–4.

    Article  Google Scholar 

  2. Luo X, Wu J, Jing S, Yan LJ. Hyperglycemic stress and carbon stress in diabetic glucotoxicity. Aging Dis. 2016;7(1):90–110.

    Article  PubMed  PubMed Central  Google Scholar 

  3. Ginter E, Simko V. Type 2 diabetes mellitus, pandemic in 21st century. Adv Exp Med Biol. 2012;771:42–50.

    Article  PubMed  Google Scholar 

  4. Lin X, Xu Y, Pan X, Xu J, Ding Y, Sun X, et al. Global, regional, and national burden and trend of diabetes in 195 countries and territories: an analysis from 1990 to 2025. Sci Rep. 2020;10(1):14790–0.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Standl E, Khunti K, Hansen TB, Schnell O. The global epidemics of diabetes in the 21st century: current situation and perspectives. Eur J Prev Cardiol. 2019;26(2):7–14.

    Article  PubMed  Google Scholar 

  6. Ahmed MH, Elwali ES, Awadalla H, Almobarak AO. The relationship between diabetic retinopathy and nephropathy in sudanese adult with diabetes: population based study. Diabetes Metab Syndr. 2017;11(1):333–6.

    Article  Google Scholar 

  7. Shweta M, Arunaksharan N. Traditional fruits of kerala: bioactive compounds and their curative potential in chronic diseases. Curr Nutr Food Sci. 2017;13(4):279–89.

    Google Scholar 

  8. Gor D, Gerber BS, Walton SM, Lee TA, Nutescu EA, Touchette DR. Antidiabetic drug use trends in patients with type 2 diabetes mellitus and chronic kidney disease: a cross-sectional analysis of the National Health and Nutrition Examination Survey. J Diabetes. 2020;12(5):385–95.

    Article  CAS  PubMed  Google Scholar 

  9. Butala MA, Kukkupuni SK, Vishnuprasad CN. Ayurvedic anti-diabetic formulation Lodhrasavam inhibits alpha-amylase, alpha-glucosidase and suppresses adipogenic activity in vitro. J Ayurveda Integr Med. 2017;8(3):145–51.

    Article  PubMed  PubMed Central  Google Scholar 

  10. Muhammad A, Annam Z, Khalid MK, Shahnaz P. Shazia Shah and Aneela K. Rivina humilis L: a potential antimicrobial and antioxidant source. J Chem Soc Pak. 2013;35(5):1384–98.

    Google Scholar 

  11. Sushama PR, Mehtab Y, Prabhakar G, Hajera S, Kaneez F. A report on Rivina humilis L. an invasive alien weed from Telangana State, India. Int J Crea & Res Thoughts. 2021;9(11):175–80.

    Google Scholar 

  12. Kokate CK. Practical Pharmacognosy. 4 ed. New Delhi: VallabhPrakashan; 1994. pp. 110–1.

    Google Scholar 

  13. Khandelwal KR. Practical pharmacognosy-techniques and experiments. Pune; NiraliPrakashan; 2000.

  14. Singleton VL, Orthofer R, Lamuela-Ravento´s RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Foline Ciocalteu reagent. Methods Enzymol. 1999;299:152–78.

    Article  CAS  Google Scholar 

  15. Rangachari B, Veeramuthu D, Savarimuthu I. Antidiabetic activity of γ-sitosterol isolated from Lippianodiflora L. in streptozotocin induced diabetic rats. Eur J Pharmacol. 2011;667:410–8.

    Article  Google Scholar 

  16. Ramesh C, Prameela Rani A. In vivo and in vitro anti-diabetic potentials of methanol extract of Tephrosia pumila against alloxan induced diabetes in experimental animals. Int J Health Sci 2019;13(3):10 – 8.

  17. Subashini TS, Prakash STS, Sunil KKN, Aiman AA, Ramzi AA, Ahmad RA. Mimosa pudica alleviates streptozotocin-induced diabetes, glycemic stress and glutathione depletion in Wistar albino rats. J King Saud Univ-Sci. 2022;34:10237–44.

    Google Scholar 

  18. Sanjay J, Gaurav B, Rakesh B, Praveen K, Avnish J, Vinod KD. Antidiabetic activity of Paspalum scrobiculatum Linn. In alloxan induced diabetic rats. J Ethnopharmacol. 2010;127:325–8.

    Article  Google Scholar 

  19. Khalili, et al. Total phenolic content and in vitro antioxidant activity of Winged Bean (Psophocarpus tetragonolobus). Pakistan J Nutr. 2013;12(5):416–22.

    Article  Google Scholar 

  20. Narayanankutty A, Kottekkat A, Mathew SE, Illam SP, Suseela IM, Raghavamenon AC. Vitamin E supplementation modulates the biological effects of omega-3 fatty acids in naturally aged rats. Toxicol Mech Methods. 2017;27(3):207–14.

    Article  CAS  PubMed  Google Scholar 

  21. Ravi M, Padmaja U, Sunil Kuma KN, Shivanada N, Alfarhan AH, et al. Protective effect of Garcinia pedunculata fruit rind in acetic acid induced ulcerative colitis. Farmacia. 2019;67(1):160–6.

    Article  Google Scholar 

  22. Ajabnor MA, Tilmisany AK. Effects of Trigonella feonumgraceum on blood glucose levels in normal and alloxan-diabetic mice. J Ethnopharmacol. 1998;22:15–49.

    Google Scholar 

  23. Onakpa MM, Asuzu IU. Histological changes and antidiabetic activities of Icacina trichantha tuber extract in beta-cells of alloxan induced diabetic rats. Asian Pac J Trop Biomed. 2013;3(8):628–33.

    Article  Google Scholar 

  24. Narayanankutty A, Palliyil DM, Kuruvilla K, Raghavamenon AC. Virgin coconut oil reverses hepatic steatosis by restoring redox homeostasis and lipid metabolism in male Wistar rats: VCO ameliorates hepatic steatosis in rats. J Sci Food Agric. 2018;98(5):1757–64.

    Article  CAS  PubMed  Google Scholar 

  25. Hafner S, Radermacher P, Frick M, Dietl P, Calzia E. Hyperglycemia, oxidative stress, and the diaphragm: a link between chronic co-morbidity and acute stress? Crit Care. 2014;18(3):149.

    Article  PubMed  PubMed Central  Google Scholar 

  26. Parasuraman S, Ching TH, Leong CH, Banik U. Antidiabetic and antihyperlipidemic effects of methanolic extract of Mimosa pudica (Fabaceae) in diabetic rats. Egypt J Basic Appl Sci. 2019;6(1):137–48.

    Google Scholar 

  27. Sheela DL, Nazeem PA, Narayanankutty A, Shylaja RM, Davis SP, James P, et al. Coconut phytocompounds inhibits polyol pathway enzymes: implication in prevention of microvascular diabetic complications. Prostaglandins Leukotrienes Essential Fatty Acids (PLEFA). 2017;127:20–4.

    Article  CAS  Google Scholar 

  28. Kaur J, Sidhu S, Chopra K, Khan MU. Protective effect of Mimosa pudica L in an L-arginine model of acute necrotising pancreatitis in rats. J Nat Med. 2016;70(3):423–34.

    Article  PubMed  Google Scholar 

  29. Ahmad G, Roghayeh R, Reza SN. Flavonoids for preserving pancreatic beta cell survival and function: a mechanistic review. Biomed & Pharmacother. 2019;111:947–57.

    Article  Google Scholar 

  30. Ahmad G. Mechanisms of antidiabetic effects of flavonoid rutin. Biomed & Pharmacother. 2017;96:305–12.

    Article  Google Scholar 

Download references

Acknowledgements

The authors of manuscript are thankful to The principal and management of East West College of Pharmacy, Bangalore for providing facilities to conduct this research work.

Funding

None.

Author information

Authors and Affiliations

  1. Department of Pharmacology, East West College of Pharmacy, No-63, I Bharath Nagar, Off Magadi Road Vishwaneedam (PO), Bangalore, 560091, India

    Ramesh C, Kaushik Ghosh & Sowmya B A

  2. Department of Pharmaceutical Chemistry, East West College of Pharmacy, Bangalore, India

    Pinkey Rawal & Soma Pramanik

Authors
  1. Ramesh C
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Kaushik Ghosh
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sowmya B A
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Pinkey Rawal
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Soma Pramanik
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

All authors have equal contributions in completing the present research work.

Corresponding author

Correspondence to Ramesh C.

Ethics declarations

Ethics approval and consent to participate

The present experimental protocol was designed according to ethical principles of CPCSEA, New Delhi and the study was approved by the Institutional Animal Ethics Committee, East West College of Pharmacy, Bangalore (Ref No.-EWCP/CPCSEA/IAEC/V/02).

Competing interests

We are by declaring that there is no conflicts interest.

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary Material 1

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

C, R., Ghosh, K., A, S.B. et al. In vivo antioxidant and hypoglycaemic potentials of Rivina humilis extract against streptozotocin induced diabetes and its complications in wistar rats. J Diabetes Metab Disord 22, 1373–1383 (2023). https://doi.org/10.1007/s40200-023-01258-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40200-023-01258-6

Keywords

Search

Navigation