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The role of the PPARG (Pro12Ala) common genetic variant on type 2 diabetes mellitus risk

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Journal of Diabetes & Metabolic Disorders Aims and scope Submit manuscript

Abstract

Background

Type 2 diabetes (T2DM) prevalence has been rapidly increasing in the last decades. T2DM pathogenesis is related to insulin resistance and beta-cell dysfunction. Peroxisome proliferator-activated receptor gamma (PPARG) is concerned about T2DM risk through the involvement in adipocyte differentiation and energy homeostasis. The present study aimed to find the risk associated with a common genetic variant (Pro12Ala) of the PPARG gene in the development of T2DM in a group of the Iranian population.

Methods

Totally, 149 patients with T2DM and 96 healthy individuals were recruited in this case–control study. The genotyping of the genetic variant was carried out using the polymerase chain reaction (PCR) followed by Sanger sequencing.

Results

No significant difference is observed between the CG and GG genotypes frequency of the PPARG variant (P = 0.17) in T2DM patient and the control groups. Furthermore, the frequency of the G allele was similar between case and control groups. The Pro12Ala variant may decrease the risk of diabetic retinopathy (DR) which was not statistically significant. Furthermore, the Pro12Ala variant caused a 27% increase in the risk of diabetes nephropathy (DN) among patients with T2DM but was not significant.

Conclusions

Our findings showed that the PPARG variant could not impact on T2DM development and its complications.

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Data availability

Data from this project will be available to share.

Abbreviations

T2DM:

Type 2 diabetes mellitus

PPARs:

Peroxisome proliferator-activated receptors

PPARG:

Peroxisome proliferator-activated receptor gamma

ADA:

American diabetes association

FPG:

Fasting plasma glucose

DR:

Diabetic retinopathy

DN:

Diabetic nephropathy

HbA1c:

Hemoglobin A1c

SNP:

Single nocleutide polymorphism

PCR:

Polymerase chain reaction

χ2:

Chi-squared test

HWE:

Hardy–weinberg equilibrium

SD:

Standard deviation

BMI:

Body mass index

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Acknowledgements

The authors would like to thank all participants who involved in this project.

Author information

Authors and Affiliations

  1. Medical Genomics Research Center, Tehran Medical Sciences, Islamic Azad University, Khaghani Avenue, Shariati St, 193951459, Tehran, Iran

    Leila Hashemian & Mandana Hasanzad

  2. Personalized Medicine Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran, Iran

    Negar Sarhangi, Hamid Reza Aghaei Meybodi & Mandana Hasanzad

  3. Department of Community Medicine, Zabol University of Medical Sciences, Zabol, Iran

    Mahdi Afshari

Authors
  1. Leila Hashemian
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  2. Negar Sarhangi
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  3. Mahdi Afshari
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  4. Hamid Reza Aghaei Meybodi
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  5. Mandana Hasanzad
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Contributions

MH managed the project and provided guidance to the research; LH and NS drafted the manuscript and performed lab genotyping; MA performed the statistical analysis; HRAM: provided clinical guidance to the research.

All authors contributed to and approved the final version of the manuscript.

Corresponding author

Correspondence to Mandana Hasanzad.

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Consent for publication

Written informed consent was obtained from all the subjects and the present study was approved by ethical committee by the ethics code of IR.IAU.TMU.REC.1395.110.

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The authors declare that there is no conflict of interest.

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Hashemian, L., Sarhangi, N., Afshari, M. et al. The role of the PPARG (Pro12Ala) common genetic variant on type 2 diabetes mellitus risk. J Diabetes Metab Disord 20, 1385–1390 (2021). https://doi.org/10.1007/s40200-021-00872-6

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  • DOI: https://doi.org/10.1007/s40200-021-00872-6

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