Association of TNF-α-308 G>A (rs1800629) polymorphism with susceptibility of metabolic syndrome



Metabolic Syndrome (MetS) mainly comprises hyperglycemia, hypertension and dyslipidemia, and has been proven to increase the risk for type 2 diabetes mellitus (T2DM) and cardiovascular disease. Studies have suggested that many factors may be involved in the pathogenesis of MetS, but tumor necrosis factor alpha (TNF- α) may play a strong role as its gene polymorphism was associated with insulin resistance and obesity. The aim of this study was to evaluate the possible association of TNF-α-308 G > A (rs1800629) polymorphism with susceptibility of metabolic syndrome.


a case-control study was conducted upon 128 participants recruited from Suez Canal University Hospital (Ismailia, Egypt), divided into the MetS group (n = 64) and the control group (n = 64). Genotyping of the TNF-α-308 G > A (rs1800629) polymorphism was performed by restriction fragment length polymorphism (PCR-RFLP).


The A allele was significantly higher among MetS patients (40%) than controls (11%) (p < 0.0001). A significant association was observed between the healthy and MetS groups under the influence of co-dominant, dominant and over-dominant genetic models (p < 0.05). Also, there were positive correlations between TNF-α-308 (G/A) polymorphism and risk factors of metabolic syndrome like body mass index (BMI); fasting blood sugar; cholesterol and low density lipoprotein (LDL) (p < 0.05). Regression analysis was done for predictors of MetS and the A allele was found to be a strong predictor (OR 2.752; 95% CI = 1.106 to 6.847; p = 0.03), as well as, BMI; triglyceride (TG); high density lipoprotein (HDL); LDL and cholesterol (p < 0.05).


TNF-α-308 G > A (rs1800629) polymorphism may be play an important role in the development of metabolic syndrome and A allele is a strong predictor in Egyptians.

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This work was done by own author funding.

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All authors have participated in the study.

Corresponding author

Correspondence to Dalia Ghareeb.

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The authors declare that they have no conflict of interest.

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All the patients were informed about the research and verbal informed consent was obtained prior to the interview.

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This research has been approved by the Medical Research Ethics Committee of the Suez Canal University Faculty of Medicine (Ismailia, Egypt) and the study has been conducted according to the principles expressed in the Helsinki Declaration.

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Key messages

• There is an evidence indicates that TNF-α 308 (G/A) polymorphism may participate in development of metabolic syndrome.

• The A allele was found to be a strong predictor as well as, BMI; TG; HDL; LDL and cholesterol for susceptibility of metabolic syndrome in Egyptian patients.

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Cite this article

Ghareeb, D., Abdelazem, A.S., Hussein, E.M. et al. Association of TNF-α-308 G>A (rs1800629) polymorphism with susceptibility of metabolic syndrome. J Diabetes Metab Disord (2021).

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  • Tumor necrosis factor alpha
  • Gene polymorphism
  • Metabolic syndrome
  • Polymerase chain reaction
  • Restriction fragment length polymorphism