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Effect of Alpinia officinarum extract on reproductive damages in streptozotocin induced diabetic male rats

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Abstract

Purpose

Infertility is one of the systemic problems in diabetic men. The purpose of the present study is investigation of the effects of the Alpinia officinarum (AO) hydro-alcoholic extract on the reproductive system damages in diabetic male rats.

Methods

Twenty four male rats were randomly assigned into 4 groups (n = 6); i.e., control, diabetic control, and diabetic rats treated orally with AO extract (200 and 500 mg kg−1). A single dose (60 mg kg-1) of streptozotocin (STZ) was injected intraperitoneally (IP) to induce diabetes. After 8 weeks of treatment, blood samples, testis, and cauda epididymis were excised to evaluate specific hormonal changes, sperm parameters, and testis morphology.

Results

Diabetic control rats showed remarkably lower body and testicular weights, testicular volumes, and sperm parameters compared with the control group (p <0.05). Diabetic control rats also exhibited significantly decreased serum testosterone and follicle stimulating hormone (FSH). Sperm parameters were considerably enhanced in diabetic animals gavaged with AO extract. Testosterone levels were significantly elevated by administrating 500 mg kg−1 AO extract to the diabetic control rats (p <0.05). The morphological assessment of testis of treatment group (500 mg kg−1) indicated remarkable differences (p <0.05) by increasing the seminiferous tubules diameter (STD) and thickness of the seminiferous epithelium (TSE) compared with diabetic control rats.

Conclusion

As demonstrated by the results, AO extract ameliorated sperm damage and improved sperm morphology besides improving histological damage in the testis in diabetic rats. In addition, the dose of 500 mg kg−1 worked more efficiently than 200 mg kg−1.

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References

  1. Öztaş E, Yılmaz TE, Güzel E, Sezer Z, Okyar A, GJSPJ Ö. Gliclazide alone or in combination with atorvastatin ameliorated reproductive damage in streptozotocin-induced type 2 diabetic male rats. Saudi Pharmaceut J. 2019;27(3):422–31.

    Article  Google Scholar 

  2. Heeba GH, Hamza AAJL. Rosuvastatin ameliorates diabetes-induced reproductive damage via suppression of oxidative stress, inflammatory and apoptotic pathways in male rats. Life Sci. 2015;141:13–9.

    Article  CAS  PubMed  Google Scholar 

  3. La Vignera S, Calogero A, Condorelli R, Lanzafame F, Giammusso B, EJME V. Andrological characterization of the patient with diabetes mellitus. Minerva Endocrinol. 2009;34(1):1–9.

    PubMed  Google Scholar 

  4. Mohasseb M, Ebied S, Yehia MAH, Hussein N. Testicular oxidative damage and role of combined antioxidant supplementation in experimental diabetic rats. J Physiol Biochem. 2011;67(2):185–94.

    Article  CAS  PubMed  Google Scholar 

  5. Kanter M, Aktas C, Erboga M. Curcumin attenuates testicular damage, apoptotic germ cell death, and oxidative stress in streptozotocin-induced diabetic rats. Mol Nutr Food Res. 2013;57(9):1578–85.

    Article  CAS  PubMed  Google Scholar 

  6. Rabbani SI, Devi K, Khanam S. Inhibitory effect of glimepiride on nicotinamide-streptozotocin induced nuclear damages and sperm abnormality in diabetic Wistar rats.Indian J. Exp. Biol. 2009;47(10):804–10.

  7. Ghanbari E, Nejati V, Najafi G, Khazaei M, Babaei M. Study on the effect of royal jelly on reproductive parameters in streptozotocin-induced diabetic rats. Int Fertil steril. 2015;9(1):113.

    CAS  Google Scholar 

  8. Ding G-L, Liu Y, Liu M-E, Pan J-X, Guo M-X, Sheng J-Z, et al. The effects of diabetes on male fertility and epigenetic regulation during spermatogenesis. Asian J Androl. 2015;17(6):948.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Afifi M, Almaghrabi OA, Kadasa NM. Ameliorative effect of zinc oxide nanoparticles on antioxidants and sperm characteristics in streptozotocin-induced diabetic rat testes. Biomed Res Int. 2015;2015:153573.

    Article  PubMed  Google Scholar 

  10. Shi G-J, Zheng J, Wu J, Qiao H-Q, Chang Q, Niu Y et al. Protective effects of Lycium barbarum polysaccharide on male sexual dysfunction and fertility impairments by activating hypothalamic pituitary gonadal axis in streptozotocin-induced type-1 diabetic male mice. Endocr J. 2017;64(9):907–22.

  11. Sookhthezari A, Alirezaei M, Kheradmand A, Dezfoulian OJSJ. Streptozotocin-induced diabetic effects on the sperm fertility parameters. Glycated Hemoglob Total Choles Mice. 2016;23(10):980–8.

    Google Scholar 

  12. Jain GC, Jangir RN. Modulation of diabetes-mellitus-induced male reproductive dysfunctions in experimental animal models with medicinal plants. Pharmacogn Rev. 2014;8(16):113.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Yildirim M, Degirmenci U, Akkapulu M, Comelekoglu U, Balli E, Ozcan TM et al. The effect of Rheum ribes L. on oxidative stress in diabetic rats. J Basic Clin Physiol Pharmacol. 2020. https://doi.org/10.1515/jbcpp-2020-0058.

  14. Almalki DA, Alghamdi SA, AI-Attar AM. Comparative study on the influence of some medicinal plants on diabetes induced by streptozocin in male rats. J Bri. 2019. https://doi.org/10.1155/2019/3596287.

  15. Rajendiran V, Natarajan V, Devaraj SN. Anti-inflammatory activity of Alpinia officinarum hance on rat colon inflammation and tissue damage in DSS induced acute and chronic colitis models. Food Sci Human Wellness. 2018;7(4):273–81.

    Article  Google Scholar 

  16. Ma Y-L, Zhao F, Yin J-T, Liang C-J, Niu X-L, Qiu Z-H, et al. Two approaches for evaluating the effects of galangin on the activities and mrna expression of seven cyp450. Molecules. 2019;24(6):1171.

    Article  PubMed Central  Google Scholar 

  17. Reid K, Wright V, Omoregie SJ. Anticancer properties of Alpinia officinarum (lesser galangal)–A mini review. Int J Adv Res. 2016;4:300–6.

    Article  CAS  Google Scholar 

  18. Honmore VS, Kandhare AD, Kadam PP, Khedkar VM, Sarkar D, Bodhankar SL, et al. Isolates of Alpinia officinarum Hance as COX-2 inhibitors: Evidence from anti-inflammatory, antioxidant and molecular docking studies. Int Immunopharmacol. 2016;33:8–17.

    Article  CAS  PubMed  Google Scholar 

  19. Eumkeb G, Sakdarat S, S S. Reversing β-lactam antibiotic resistance of Staphylococcus aureus with galangin from Alpinia officinarum Hance and synergism with ceftazidime. Phytomedicine. 2010;18(1):40–5.

    Article  CAS  PubMed  Google Scholar 

  20. Honmore VS, Rojatkar SR, Nawale LU, Arkile MA, Khedkar VM, Natu AD, et al. In vitro and ex vivo antitubercular activity of diarylheptanoids from the rhizomes of Alpinia officinarum Hance. Nat Prod Res. 2016;30(24):2825–30.

    Article  CAS  PubMed  Google Scholar 

  21. Köse LP, Gülcin I, Gören AC, Namiesnik J, Martinez-Ayala AL, Gorinstein S. LC–MS/MS analysis, antioxidant and anticholinergic properties of galanga (Alpinia officinarum Hance) rhizomes. Ind Crop Prod. 2015;74:712–21.

    Article  Google Scholar 

  22. Khorasani MA. Makhzan ul- Advia. 3rd ed. Tehran university publications; 1996. pp. 479–80

  23. Ahangarpour A, Heidari H, Junghani MS, Absari R, Khoogar M, Ghaedi E. Effects of hydroalcoholic extract of Rhus coriaria seed on glucose and insulin related biomarkers, lipid profile, and hepatic enzymes in nicotinamide-streptozotocin-induced type II diabetic male mice. Res Pharm Sci. 2017;12(5):416.

    Article  PubMed  PubMed Central  Google Scholar 

  24. Nickavar B, Esbati N. Evaluation of the antioxidant capacity and phenolic content of three Thymus species. J Acupunct Meridian Stud. 2012;5(3):119–25.

    Article  PubMed  Google Scholar 

  25. Samie A, Sedaghat R, Baluchnejadmojarad T, Roghani M. Hesperetin, a citrus flavonoid, attenuates testicular damage in diabetic rats via inhibition of oxidative stress, inflammation, and apoptosis. Life Sci. 2018;210:132–9.

    Article  CAS  PubMed  Google Scholar 

  26. Kumar R, Pate DK, Prasad SK, Sairam K, Hemalatha S. Antidiabetic activity of alcoholic leaves extract of Alangium lamarckii Thwaites on streptozotocin–nicotinamide induced type 2 diabetic rats. Asian Pac J Trop Med. 2011;4(11):904–9.

    Article  PubMed  Google Scholar 

  27. Parmar GR, Pundarikakshudu K, Balaraman R. Antidiabetic and antihyperlipidemic activity of Euphorbia thymifolia L. extracts on streptozotocin-nicotinamide induced type 2 diabetic rats. J Appl Pharmaceut Sci. 2017;7(08):078–84.

    CAS  Google Scholar 

  28. Furman BL. Streptozotocin-induced diabetic models in mice and rats. Curr Protoc Pharmacol. 2015;70(1):5.47.1–5.20.

    Article  Google Scholar 

  29. Weinberg E, Maymon T, Moses O, Weinreb M. Streptozotocin-induced diabetes in rats diminishes the size of the osteoprogenitor pool in bone marrow. Diabetes Res Clin Pract. 2014;103(1):35–41.

    Article  CAS  PubMed  Google Scholar 

  30. Ahangarpour A, Oroojan AA, Radan M. Effect of aqueous and hydro-alcoholic extracts of lettuce (Lactuca sativa) seed on testosterone level and spermatogenesis in NMRI mice. Iran J Reprod Med. 2014;12(1):65.

    PubMed  PubMed Central  Google Scholar 

  31. Haredy SA, Imam TS, Ahmed-Farid OA. Combination of Ficus carica leaves extract and ubiquinone in a chronic model of lithium induce reproductive toxicity in rats: Hindrance of oxidative stress and apoptotic marker of sperm cell degradation. JJPBS. 2017;12:64–73.

    Google Scholar 

  32. Keyhanmanesh R, Hamidian G, Alipour MR, Ranjbar M, Oghbaei H. Protective effects of sodium nitrate against testicular apoptosis and spermatogenesis impairments in streptozotocin-induced diabetic male rats. Life Sci. 2018;211:63–73.

    Article  CAS  PubMed  Google Scholar 

  33. Ommati MM, Heidari R, Jamshidzadeh A, Zamiri MJ, Sun Z, Sabouri S, et al. Dual effects of sulfasalazine on rat sperm characteristics, spermatogenesis, and steroidogenesis in two experimental models. Toxicol Lett. 2018;284:46–55.

    Article  CAS  PubMed  Google Scholar 

  34. Navarro-Casado L, Juncos-Tobarra M, Chafer-Rudilla M, De Onzono LÍ, Blazquez-Cabrera J, Miralles-Garcia JJ. Effect of experimental diabetes and STZ on male fertility capacity. Study in Rats. J Androl. 2010;31(6):584–92.

    Article  CAS  PubMed  Google Scholar 

  35. Halvaei I, Roodsari HRS, Harat ZN. Acute effects of Ruta graveolens L. on sperm parameters and DNA integrity in rats. J Reprod Infertil. 2012;13(1):33.

    PubMed  PubMed Central  Google Scholar 

  36. Ahangarpour A, Oroojan AA, Heidari H, Ehsan G, Nooshabadi R, Nooshabadi MRR. Effects of hydro-alcoholic extract of Rhus coriaria (Sumac) seeds on reproductive complications of nicotinamide-streptozotocin induced type-2 diabetes in male mice. World J Mens Health. 2014;32(3):151–8.

    Article  PubMed  PubMed Central  Google Scholar 

  37. Khaneshi F, Nasrolahi O, Azizi S, Nejati V. Sesame effects on testicular damage in streptozotocin-induced diabetes rats. Avicenna J Phytomed. 2013;3(4):347.

    PubMed  PubMed Central  Google Scholar 

  38. Soudamani S, Yuvaraj S, Malini T, Balasubramanian K. Experimental diabetes has adverse effects on the differentiation of ventral prostate during sexual maturation of rats. Anat Rec A Discov Mol Cell Evol Biol. 2005;287(2):1281–9.

    Article  PubMed  Google Scholar 

  39. Shoorei H, Khaki A, Khaki AA, Hemmati AA, Moghimian M, Shokoohi M, et al. The ameliorative effect of carvacrol on oxidative stress and germ cell apoptosis in testicular tissue of adult diabetic rats. Biomed Pharmacother. 2019;111:568–78.

    Article  CAS  PubMed  Google Scholar 

  40. Kaushik D, Yadav J, Kaushik P, Sacher D, Rani R. Current pharmacological and phytochemical studies of the plant Alpinia galanga. Zhong Xi Yi Jie He Xue Bao. 2011;9(10):1061–5.

    Article  CAS  PubMed  Google Scholar 

  41. Aloud AA, Chinnadurai V, Govindasamy C, Alsaif MA, Al-Numair KS. Galangin, a dietary flavonoid, ameliorates hyperglycaemia and lipid abnormalities in rats with streptozotocin-induced hyperglycaemia. Pharm Biol. 2018;56(1):302–8.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Saggu S, Sakeran MI, Zidan N, Tousson E, Mohan A, Rehman H, et al. Ameliorating effect of chicory (Chichorium intybus L.) fruit extract against 4-tert-octylphenol induced liver injury and oxidative stress in male rats. Food Chem Toxicol. 2014;72:138–46.

    Article  CAS  PubMed  Google Scholar 

  43. Javanmardi J, Stushnoff C, Locke E, Vivanco JM. Antioxidant activity and total phenolic content of Iranian Ocimum accessions. Food Chem. 2003;83(4):547–50.

    Article  CAS  Google Scholar 

  44. Abbas ZK, Saggu S, Sakeran MI, Zidan N, Rehman H, Ansari AA. Phytochemical, antioxidant and mineral composition of hydroalcoholic extract of chicory (Cichorium intybus L.) leaves. Saudi J Biol Sci. 2015;22(3):322–6.

    Article  CAS  PubMed  Google Scholar 

  45. Kolangi F, Shafi H, Memariani Z, Kamalinejad M, Bioos S, Jorsaraei SGA, et al. Effect of Alpinia officinarum Hance rhizome extract on spermatogram factors in men with idiopathic infertility: A prospective double-blinded randomised clinical trial. Randomized Controlled Trial. 2019;51(1):e13172.

    Google Scholar 

  46. Negm SH, Ragheb EM. Effect of (m) hance on sex hormones and certain biochemical parameters of adult male experimental rats. J Sci Dent. 2019;10(9):315–22.

    Google Scholar 

  47. Mazaheri M, Shahdadi V, Boron AN. Molecullar and biochemical effect of alcohlic extract of Alpinia galanga on rat spermatogenesis process. Iran J Reprod Med. 2014;12(11):765.

    PubMed  PubMed Central  Google Scholar 

  48. Qureshi S, Shah A, Ageel AM. Toxicity studies on Alpinia galanga and Curcuma longa. Planta Med. 1992;58(02):124–7.

    Article  CAS  PubMed  Google Scholar 

  49. Fedder MD, Jakobsen HB, Giversen I, Christensen LP, Parner ET, Fedder J. An extract of pomegranate fruit and galangal rhizome increases the numbers of motile sperm: a prospective, randomised, controlled, double-blinded trial. Randomized Controlled Trial. Plos One. 2014;9(10). https://doi.org/10.1371/journal.pone.0108532.

  50. Kolangi F, Shafi H, Memariani Z, Kamalinejad M, Naeimi M, Mozaffarpur SAJT et al. Improvement in semen quality and occurrence of pregnancy treated with an herbal medication: a case report. Traditional and Integrative Medicine. 2019;4(3):123–29.

  51. Zhao H, Xu S, Wang Z, Li Y, Guo W, Lin C, et al. Repetitive exposures to low-dose X-rays attenuate testicular apoptotic cell death in streptozotocin-induced diabetes rats. Toxicol Lett. 2010;192(3):356–64.

    Article  CAS  PubMed  Google Scholar 

  52. Hussein Z, Al-Qaisi J. Effect of diabetes mellitus type 2 on pituitary gland hormones (FSH, LH) in men and women in Iraq. Al-Nahrain J Sci. 2012;15(3):75–9.

    Google Scholar 

  53. Jelodar G, Khaksar Z, Pourahmadi M. Endocrine profile and testicular histomorphometry in adult rat offspring of diabetic mothers. J Physiol Sci. 2009;59(5):377.

    Article  CAS  PubMed  Google Scholar 

  54. Ballester J, Muñoz MC, Domínguez J, Rigau T, Guinovart JJ, Rodríguez-Gil JE. Insulin-dependent diabetes affects testicular function by FSH-and LH-linked mechanisms. J Androl. 2004;25(5):706–19.

    Article  CAS  PubMed  Google Scholar 

  55. Ebokaiwe AP, Ijomone OM, Osawe SO, Chukwu CJ, Ejike CE, Zhang G, et al. Alteration in sperm characteristics, endocrine balance and redox status in rats rendered diabetic by streptozotocin treatment: attenuating role of Loranthus micranthus. Redox Rep. 2018;23(1):194–205.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  56. Kianifard D, Sadrkhanlou RA, Hasanzadeh S. The ultrastructural changes of the sertoli and leydig cells following streptozotocin induced diabetes. Iran J Basic Med Sci. 2012;15(1):623.

    PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This research project was approved and financially supported by Qom University of medical sciences (Qom, Iran). The authors deeply thank Miss Azam Khalaj and Danesh laboratory for their technical support.

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Authors and Affiliations

  1. Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran

    Hamid Heidari

  2. Department of Anatomical Sciences, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

    Maasoume Abdollahi

  3. Department of Chemistry, University of Massachusetts Boston, Boston, MA, USA

    Sima Khani

  4. Department of Iranian Traditional Medicine, School of Medicine, Qom University of Medical Sciences, Qom, Iran

    Fatemeh Nojavan

  5. Neuroscience Research Center, Qom University of Medical Sciences, Pardis Campus, Ghadir Blvd, Qom, Iran

    Samira Khani

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  1. Hamid Heidari
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Correspondence to Samira Khani.

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Heidari, H., Abdollahi, M., Khani, S. et al. Effect of Alpinia officinarum extract on reproductive damages in streptozotocin induced diabetic male rats. J Diabetes Metab Disord 20, 77–85 (2021). https://doi.org/10.1007/s40200-020-00711-0

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