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Attenuating properties of Rubus fruticosus L. on oxidative damage and inflammatory response following streptozotocin-induced diabetes in the male Wistar rats

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Abstract

Purpose

Diabetes mellitus is a prevalent metabolic disorder that entails numerous complications in various organs. In current era, different types of diseases are being treated by the applications of herbs. The present study is aimed at investigating the anti-inflammatory and antioxidant effects of the Rubus fruticosus hydroethanolic extracts (RFHE) in the streptozotocin (STZ)-induced diabetic rats.

Methods

At this experimental research, male Wistar rats with the weight of 220 ± 20 g, were categorized randomly into five groups of vehicles as control, STZ (60 mg kg− 1 of body weight, intraperitoneally (i.p.)) and RFHE (50, 100 and 200 mg kg− 1, i.p.). In the last stage (end of week 4) of the experiment, after being euthanized, the blood samples of the rats were collected for measuring malondialdehyde (MDA), glutathione (GSH), total antioxidant status (TAS) as well as inflammatory markers like tumor necrosis factor (TNF)-α, interleukin (IL)-6 and C-reactive protein (CRP).

Results

Data from this study was revealed that diabetes causes oxidative damage and consequently the serum level of inflammatory markers rises. RFHE was shown to be significantly correlated with lowering the level of MDA, TNF-α, IL-6 and CRP of diabetic rats. Moreover, RFHE significantly elevated the GSH and TAS serum levels in diabetic rats when compared with STZ group.

Conclusions

RFHE might have anti-diabetic properties; this outcome may be mediated by high antioxidant and anti-inflammatory effects.

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Funding

The authors have declared that there were no funding received for this study.

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Authors and Affiliations

  1. Department of Biology, Faculty of Basic Sciences, Bu-Ali Sina University, Hamedan, Iran

    Naser Mirazi

  2. Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

    Abdolkarim Hosseini

Authors
  1. Naser Mirazi
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  2. Abdolkarim Hosseini
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Correspondence to Naser Mirazi.

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Conflict of interest disclosure

The authors wish to declare that there were no competing interests.

Research involving human participants and/or animals

All the ethical considerations of working with the animals were accounted for (Guide for the Care and Use of Laboratory Animals, 8th edition, National Academies Press). Furthermore, the Ethics Committee of Bu-Ali Sina University, confirmed the validity of this project (IR.BASU.REC.1397.036).

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Mirazi, N., Hosseini, A. Attenuating properties of Rubus fruticosus L. on oxidative damage and inflammatory response following streptozotocin-induced diabetes in the male Wistar rats. J Diabetes Metab Disord 19, 1311–1316 (2020). https://doi.org/10.1007/s40200-020-00649-3

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  • DOI: https://doi.org/10.1007/s40200-020-00649-3

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