Investigation of oxidative stress markers and antioxidant enzymes activity in newly diagnosed type 2 diabetes patients and healthy subjects, association with IL-6 level
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Oxidative stress and inflammation play an important role in the pathogenesis of diabetes and its complication. In this study, we aimed to evaluate and compare oxidative stress markers and antioxidant enzymes activity, as well as Interleukin 6 (IL-6) level in newly diagnosed type 2 diabetes mellitus (T2DM) patients and healthy subjects.
Material and methods
30 newly diagnosed type 2 diabetes patients and 30 healthy subjects (age and sex matched) were recruited in this study. After anthropometric parameters measurement, blood sample were collected from all participant. Serum and plasma were isolated. Biochemical parameters were evaluated in serum. Plasma was used to measure malondialdehyde (MDA), total oxidant status (TOS), total antioxidant capacity (TAC) levels, as well as superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity. Also, IL-6 level was investigated in plasma using ELISA method.
MDA and TOS levels were significantly higher in T2DM patients than the control group (p < 0.05). However, TAC and SOD were significantly lower in T2DM as compared with healthy subjects (p < 0.05). Also, IL-6 level was higher in T2DM in comparison to healthy subjects (p = 0.004). Furthermore, there was a significant positive correlation between IL-6 with MDA (p = 0.031, r = 0.482) and TOS (p < 0.001, r = 0.744). In addition, a negative correlation was observed between IL-6 and SOD activity (p = 0.002, r = −0.660).
Reducing oxidative stress and inflammation could be effective in improvement of T2DM.
KeywordsType 2 diabetes mellitus (T2DM) Malondialdehyde (MDA) Total oxidant status (TOS) Total antioxidant capacity (TAC) Superoxide dismutase (SOD) and glutathione peroxidase (GPx) Interleukin 6 (IL-6)
We would like to thank staff of Hamadan Health Center for providing assistance.
This study was financially supported by Hamadan University of Medical Sciences (Grant Number: 9612087760).
Compliance with ethical standards
Conflict of interest
The authors declare no conflict of interest.
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