Effects of moderate-intensity continuous training and high-intensity interval training on serum levels of Resistin, Chemerin and liver enzymes in Streptozotocin-Nicotinamide induced Type-2 diabetic rats
- 42 Downloads
The aim of this study was to investigate the effects of of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) in serum resistin, chemerin, insulin, liver enzymes and lipid profiles levels.
24 Wistar rats with mean weight of 200 ± 50 g were randomly assigned to non-diabetic rats (ND-Cnt), diabetic control (D-Cnt), diabetic training groups. The diabetic training group received 10 weeks of HIIT (D-HIIT) and MICT (D-MICT) following the induction of diabetes. Evaluating resistin, chemerin and insulin hormones levels through ELISA. FBS and liver enzyme levels were measured by biochemical kits.
HIIT and MICT resulted in a significant decrease in resistin, chemerin and fasting blood glucose (P < 0.05) compared to the D-Cnt (P < 0.05). Serum values of FBS, lipid profiles and liver enzyme (P < 0.05) decreased significantly more in the HIIT group compared with the MICT group (P < 0.05). As well as, the resistin level positively and significantly associated with values of ALT and chemerin level positively and significantly associated with values of ALT, ALP and AST in all rat (P < 0.05).
In general, our findings demonstrated that the HIIT leads to better improvements in serum liver enzyme levels, FBS and lipid profiles compared to MICT. HIIT therefore appears to be an important time-efficient treatment for treatment with type 2 diabetes rats.
KeywordsDiabetic Resistin Chemerin Liver enzyme Moderate-intensity continuous training (MICT) High-intensity interval training (HIIT)
Aspartate trans aminase
High-intensity interval training
Moderate-intensity continuous training
Non diabetic rats
Diabetic control rats
Diabetic rats that received moderate-intensity continuous training
Diabetic rats that were treated with high-intensity interval training
High-density lipoprotein cholesterol
Low-density lipoprotein cholesterol
MP, EKH, AS, and ART were responsible for the study concept and design. MP, EKH, AS, and ART contributed to data acquisition. ELAMM, LFB, EKT, CAP and EAVM assisted with data analysis and interpretation of findings. MP, EKH, AS, and ART drafted the manuscript. All authors provided critical revision of the manuscript for important intellectual content and approved final version for publication.
Funding for this study was provided by Arak University. The funding sources had no other role other than financial support.
Compliance with ethical standards
The authors declare that they have no competing interests.
All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethical Committee of Experimental Animals of the Faculty of Medicine in Inonu Univer-sity, at which the studies were conducted.
Consent for publication
- 7.Khalili M, Shuhart MC, Lombardero M, Feld JJ, Kleiner DE, Chung RT, et al. Relationship between metabolic syndrome, alanine aminotransferase levels, and liver disease severity in a multiethnic north American cohort with chronic hepatitis B. Diabetes Care. 2018:dc180040.Google Scholar
- 12.Ahmed OM, Mahmoud AM, Abdel-Moneim A, Ashour MB. Antidiabetic effects of hesperidin and naringin in type 2 diabetic rats. Diabetol Croat. 2012;41:2.Google Scholar
- 14.Asalah AK, Alsayed MA, Al-Aleem DIA, El Malkey NF. Serum resistin, vaspin and chemerin in rats with non alcoholic fatty liver disease: correlation with metabolic and haemostatic parameters. Basic Sciences of Medicine. 2014;3(4):69–84.Google Scholar
- 16.Hong C, Yan-jiao W, Yi J, Cheng Z, YA-qun G. Relationship between chemerin and metabolic syndrome. Journal of Xi'an Jiaotong University (Medical Sciences). 2011;6:013.Google Scholar
- 21.Hansen D, Dendale P, Jonkers RA, Beelen M, Manders RJ, Corluy L, et al. Continuous low- to moderate-intensity exercise training is as effective as moderate- to high-intensity exercise training at lowering blood HbA(1c) in obese type 2 diabetes patients. Diabetologia. 2009;52(9):1789–97.PubMedPubMedCentralCrossRefGoogle Scholar
- 22.Cassidy S, Thoma C, Hallsworth K, Parikh J, Hollingsworth KG, Taylor R, et al. High intensity intermittent exercise improves cardiac structure and function and reduces liver fat in patients with type 2 diabetes: a randomised controlled trial. Diabetologia. 2016;59(1):56–66.PubMedCrossRefGoogle Scholar
- 24.Ephraim RK, Acheampong E, Swaray SM, Odame Anto E, Agbodzakey H, Adoba P, et al. Developing a modified low-density lipoprotein (M-LDL-C) Friedewald’s equation as a substitute for direct LDL-C measure in a Ghanaian population: a comparative study. Journal of Lipids. 2018;2018:1–9.CrossRefGoogle Scholar
- 25.Husna F, Suyatna FD, Arozal W, Poerwaningsih EH. Antihyperglycemia effect and antioxidant capacity of Murraya koenigii in nicotinamide-Streptozotocin induced diabetic rats. Acta Biomedica Indonesiana. 2018.Google Scholar
- 26.Ranasinghe C, Hills A, Constantine G, Finlayson G, Katulanda P, King N. Study protocol: a randomised controlled trial of supervised resistance training versus aerobic training in Sri Lankan adults with type 2 diabetes mellitus: SL-DART study. BMC Public Health. 2018;18(1):176.PubMedPubMedCentralCrossRefGoogle Scholar
- 29.Schmatz R, Perreira LB, Stefanello N, Mazzanti C, Spanevello R, Gutierres J, et al. Effects of resveratrol on biomarkers of oxidative stress and on the activity of delta aminolevulinic acid dehydratase in liver and kidney of streptozotocin-induced diabetic rats. Biochimie. 2012;94(2):374–83.PubMedCrossRefGoogle Scholar
- 34.Liu G, Wang X-H. Research advances in the effects of excise and diet on LPL and its mechanism. Sheng li ke xue jin zhan [Progress in physiology]. 2014;45(2):87–92.Google Scholar
- 38.Yang M, Yang G, Dong J, Liu Y, Zong H, Liu H, et al. Elevated plasma levels of chemerin in newly diagnosed type 2 diabetes mellitus with hypertension. Journal of investigative medicine : the official publication of the American Federation for Clinical Research. 2010;58(7):883–6.CrossRefGoogle Scholar
- 39.Buechler C. Chemerin in liver diseases. Endocrinology & Metabolic Syndrome. 2014;3:4.Google Scholar
- 42.Palsamy P, Sivakumar S, Subramanian S. Resveratrol attenuates hyperglycemia-mediated oxidative stress, proinflammatory cytokines and protects hepatocytes ultrastructure in streptozotocin–nicotinamide-induced experimental diabetic rats. Chem Biol Interact. 2010;186(2):200–10.PubMedCrossRefGoogle Scholar
- 44.Hosseini M, Afsariehee B, Reza M. The effect of high intensity interval training and aloe Vera consumption on resistin and insulin resistance index in diabetic rat. Feyz Journal of Kashan University of Medical Sciences. 2018;22(4):370–8.Google Scholar