Effects of moderate-intensity continuous training and high-intensity interval training on serum levels of Resistin, Chemerin and liver enzymes in Streptozotocin-Nicotinamide induced Type-2 diabetic rats

  • Parastesh MohammadEmail author
  • Khosravi Zadeh Esfandiar
  • Saremi Abbas
  • Rekabtalae Ahoora
Research article



The aim of this study was to investigate the effects of of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) in serum resistin, chemerin, insulin, liver enzymes and lipid profiles levels.


24 Wistar rats with mean weight of 200 ± 50 g were randomly assigned to non-diabetic rats (ND-Cnt), diabetic control (D-Cnt), diabetic training groups. The diabetic training group received 10 weeks of HIIT (D-HIIT) and MICT (D-MICT) following the induction of diabetes. Evaluating resistin, chemerin and insulin hormones levels through ELISA. FBS and liver enzyme levels were measured by biochemical kits.


HIIT and MICT resulted in a significant decrease in resistin, chemerin and fasting blood glucose (P < 0.05) compared to the D-Cnt (P < 0.05). Serum values of FBS, lipid profiles and liver enzyme (P < 0.05) decreased significantly more in the HIIT group compared with the MICT group (P < 0.05). As well as, the resistin level positively and significantly associated with values of ALT and chemerin level positively and significantly associated with values of ALT, ALP and AST in all rat (P < 0.05).


In general, our findings demonstrated that the HIIT leads to better improvements in serum liver enzyme levels, FBS and lipid profiles compared to MICT. HIIT therefore appears to be an important time-efficient treatment for treatment with type 2 diabetes rats.


Diabetic Resistin Chemerin Liver enzyme  Moderate-intensity continuous training (MICT) High-intensity interval training (HIIT) 



Alanine aminotransferase


Gamma –glutamyltransferase


Aspartate trans aminase


Alkaline phosphatase


High-intensity interval training


Moderate-intensity continuous training


Non diabetic rats


Diabetic control rats


Diabetic rats that received moderate-intensity continuous training


Diabetic rats that were treated with high-intensity interval training




High-density lipoprotein cholesterol


Low-density lipoprotein cholesterol


Authors’ contributions

MP, EKH, AS, and ART were responsible for the study concept and design. MP, EKH, AS, and ART contributed to data acquisition. ELAMM, LFB, EKT, CAP and EAVM assisted with data analysis and interpretation of findings. MP, EKH, AS, and ART drafted the manuscript. All authors provided critical revision of the manuscript for important intellectual content and approved final version for publication.


Funding for this study was provided by Arak University. The funding sources had no other role other than financial support.

Compliance with ethical standards

Competing interests

The authors declare that they have no competing interests.

Ethical approval

All procedures performed in studies involving animals were in accordance with the ethical standards of the Ethical Committee of Experimental Animals of the Faculty of Medicine in Inonu Univer-sity, at which the studies were conducted.

Consent for publication

Not applicable.


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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Parastesh Mohammad
    • 1
    Email author
  • Khosravi Zadeh Esfandiar
    • 2
  • Saremi Abbas
    • 2
  • Rekabtalae Ahoora
    • 1
  1. 1.Faculty of Sport Sciences, Department of Sports Physiology and PathologyArak UniversityArakIran
  2. 2.Faculty of Sport Sciences, Department of Sports ManagementArak UniversityArakIran

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