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Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil

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Abstract

Background

Biological activities of Pistacia atlantica have been investigated for few decades. The fruit oil of the plant has been used for treatment of wounds, inflammation, and other ailments in Traditional Persian Medicine (TPM).

Objectives

The main objectives of this study were to analyze the chemical composition of Pistacia atlantica fruit oil and to study wound healing and anti-inflammatory effects of oil-absorbed bacterial cellulose in an in vivo burn wound model.

Method

Bacterial cellulose membrane was prepared from Kombucha culture and Fourier-transform infrared was used to characterize the bacterial cellulose. Cold press technique was used to obtain Pistacia atlantica fruit oil and the chemical composition was analyzed by gas chromatography. Bacterial cellulose membrane was impregnated with the Pistacia atlantica fruit oil. Pistacia atlantica hydrogel was prepared using specific Carbopol. Burn wound model was used to evaluate in vivo wound healing and anti-inflammatory effects of the wound dressings containing either silver sulfadiazine as positive control, Pistacia atlantica hydrogel or bacterial cellulose membrane coated with the Pistacia atlantica fruit oil. Blank dressing was used as negative control.

Results

FT-IR analysis showed that the structure of the bacterial cellulose corresponded with the standard FT-IR spectrum. The major components of Pistacia atlantica fruit oil constituted linoleic acid (38.1%), oleic acid (36.9%) and stearic acid (3.8%). Histological analysis showed that bacterial cellulose coated with fruit oil significantly decreased the number of neutrophils as a measure of inflammation compared to either negative control or positive control (p < 0.05). Wound closure occurred faster in the treated group with fruit oil-coated bacterial cellulose compared to the other treatments (p < 0.05).

Conclusion

The results showed that bacterial cellulose coated with Pistacia atlantica fruit oil can be a potential bio-safe dressing for wound management.

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Data Availability

The data used to support the findings of this study are available from the corresponding author upon request.

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Acknowledgements

The authors would like to sincerely thank Dr. Mohsen Amini for his assistance in GC analysis. We appreciate the collaboration of Dr. Gholamreza Amin for identifying the plant species and assigning scientific name.

Funding

National Institutes of Medical Research Development (NIMAD 958943).

Author information

Authors and Affiliations

  1. Department of Drug and Food Control, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Navid Mirmohammadsadegh, Marzieh Shakoori, Hanieh Nobari Moghaddam, Ramtin Farhadi & Mohsen Amin

  2. Department of Pharmaceutical Biotechnology, and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran

    Ahmad Reza Shahverdi

  3. Pharmaceutical Quality Assurance Research Center, The Institute of Pharmaceutical Sciences (TIPS), Tehran University of Medical Sciences, Room No. 1-221, Faculty of Pharmacy, 16th Azar Street, Tehran, Iran

    Mohsen Amin

Authors
  1. Navid Mirmohammadsadegh
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  2. Marzieh Shakoori
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  3. Hanieh Nobari Moghaddam
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  6. Mohsen Amin
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Corresponding author

Correspondence to Mohsen Amin.

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Ethical approval

An animal study was conducted based on ethical guidelines of Iran National Committee for Ethics in Biomedical Research (IR.TUMS.MEDICINE.REC.1399.1194).

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Informed consent was obtained from all individual participants included in the study.

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Mirmohammadsadegh, N., Shakoori, M., Moghaddam, H.N. et al. Wound healing and anti-inflammatory effects of bacterial cellulose coated with Pistacia atlantica fruit oil. DARU J Pharm Sci 30, 1–10 (2022). https://doi.org/10.1007/s40199-021-00405-9

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