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Vitamin B combination reduces fluconazole toxicity in Wistar rats

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Abstract

Background

The major adverse effect associated with systemic administration of Fluconazole (FLZ), is hepatic toxicity. FLZ is most commonly used antifungal drug in treatment of invasive fungal infections.

Methods

FLZ toxicity was challenged by individual and in combination of three vitamins (B1, B2 B3). Animals were divided nine groups with six animals in each group. FLZ, at a dose of 50 mg/kg b.w, was orally administered for 90 days in experimental animals. Vitamins as individual or in combination was administered concomitantly to challenge or alleviate the toxicity of FLZ. They were sacrificed at the end of protocol for biochemical and histopathology analysis. Focus was made to observe the role of these micro nutrient’s (vitamins) on liver for alteration in of pathological and physiological effects by FLZ in the Wistar albino rats.

Results

Combination of vitamin B1 + B2 + B3 in FLZ induced toxicity was able to restore the level of alkaline phosphatase (ALP) near to normal but with high level of ALP in B1 Control group. Aspartate aminotransferase (AST) was restored to normal in FLZ + B1, FLZ + B2 and FLZ + B1 + B2 + B3 groups and vice versa in FLZ + B3 group animals. Further the level of alanine aminotransferase (ALT) was restored to normal in FLZ + B3 animals. There were no significant changes found in total bilirubin (TBI), and direct bilirubin (DBI) as compare to normal control. Histopathological studies on animals’ studies validated the serological results in normalizing the cellular architecture of liver.

Conclusions

Restoration of altered biochemical parameters and cellular architecture of hepatocytes by different combination of these vitamins proves the chemo preventive potential of these micro nutrients’ in FLZ toxicity.

Vitamin B combination attenuates fluconazole toxicity.

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Acknowledgements

This project was funded by the Deanship of Scientific Research (DSR) at King Abdulaziz University, Jeddah, under the grant no. G-389.130-38. The authors, therefore, acknowledge with thanks DSR for their technical and financial support.

The authors thank Kaleemuddin Mohammed, Ph.D. research scholar at our department for his assistance in performing experiments and drafting this manuscript.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Science, King Abdulaziz University, P.O. Box: 80200, Zip Code: 21589, Jeddah, Kingdom of Saudi Arabia

    Fahad A. Al-Abbasi, Saida Sadath, Gauhar Mushtaq & Firoz Anwar

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  1. Fahad A. Al-Abbasi
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  2. Saida Sadath
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  3. Gauhar Mushtaq
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Correspondence to Fahad A. Al-Abbasi or Firoz Anwar.

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Al-Abbasi, F.A., Sadath, S., Mushtaq, G. et al. Vitamin B combination reduces fluconazole toxicity in Wistar rats. DARU J Pharm Sci 27, 525–531 (2019). https://doi.org/10.1007/s40199-019-00252-9

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  • DOI: https://doi.org/10.1007/s40199-019-00252-9

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