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Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments

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Acta Metallurgica Sinica (English Letters) Aims and scope

Abstract

Porous titanium scaffold with suitable porous architecture exhibits enormous potentials for bone defect repairs. However, insufficient osteointegration and osteoinduction still remain to open as one of the major problems to achieve satisfactory therapeutic effect. To solve this problem, many studies have been carried out to improve the bioactivity of porous titanium scaffold by surface modifications. In this study, porous Ti6Al4V scaffolds were fabricated using additive manufacturing technique. Porous architectures were built up based on a diamond pore structure unit. Alkali–acid-heat (AH) treatment was applied to create a TiO2 layer on the porous Ti6Al4V scaffold (AH-porous Ti6Al4V). Subsequently, a hydrothermal treatment was employed to enable the formation of HA coating with nanopillar-like morphology on the alkali–acid-heat-treated surface (HT/AH-porous Ti6Al4V). The effects of surface modifications on apatite-forming ability, protein adsorption, cell attachment, cell proliferation and osteogenic gene expression were studied using apatite-forming ability test, protein adsorption assay and in vitro cell culture assay. It was found that the HT/AH-porous Ti6Al4V exhibited the highest apatite formation ability and best affinity to fibronectin and vitronectin. In vitro studies indicated that the mesenchymal stem cells (MSCs) cultured on the HT/AH-porous Ti6Al4V presented improved adhesion and differentiation compared with the porous Ti6Al4V and AH-porous Ti6Al4V.

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Acknowledgements

This work was financially supported by the National Basic Research Program of China (Grant No. 2016YFC1102000), the National Natural Science Foundation of China (Grant Nos. 81672139 and 81702129), the China Postdoctoral Science Foundation (No. 171479), the Doctor Initial Foundation of Liaoning Province (No. 20170520017) and the Affiliated Zhongshan Hospital of Dalian University (No. DLDXZSYY-DK201701).

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Zhongshan Hospital of Dalian University, Dalian, 116001, China

    Jun-Lei Li, Shuai Wang, Fang Cao, Xiao-Wei Wei, Zhen-Hua Zhao, Xiao-Jie Dou, Wei-Ting Yu & De-Wei Zhao

  2. Orthopaedic Institute, Medical College, Soochow University, Suzhou, 215006, China

    Xiao Lin

  3. Institute of Metal Research, Chinese Academy of Sciences, Shenyang, 110016, China

    Ke Yang

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  1. Jun-Lei Li
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Correspondence to Ke Yang or De-Wei Zhao.

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Li, JL., Wang, S., Cao, F. et al. Fabrication and Characterization of Nanopillar-Like HA Coating on Porous Ti6Al4V Scaffold by a Combination of Alkali–Acid-Heat and Hydrothermal Treatments. Acta Metall. Sin. (Engl. Lett.) 32, 1075–1088 (2019). https://doi.org/10.1007/s40195-019-00920-4

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