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In Vitro Biocompatibility of MC3T3-E1 Osteoblast-like Cells on Arg-Gly-Asp Acid Peptides Immobilized Graphite-like Carbon Coating on Carbon/Carbon Composites

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Acta Metallurgica Sinica (English Letters) Aims and scope

Abstract

Carbon/carbon (C/C) composites were deposited with graphite-like carbon (GLC) coating, and then, Arg-Gly-Asp acid (RGD) peptides were successfully immobilized onto the functionalized GLC coating. GLC coating was utilized to prevent carbon particles releasing and create a uniform surface condition for C/C composites. RGD peptides were utilized to improve biocompatibility of GLC coating. Surface chemical characterizations of functionalized GLC coating were detected by contact angle measurement, X-ray photoelectron spectroscopy and Raman spectra. Optical morphology of GLC coatings was observed by confocal laser scanning microscopy. In vitro biological performance was determined using samples seeded with MC3T3-E1 osteoblast-like cells and cultured for 1 week. Surface characterizations and morphological analysis indicated that C/C composites were covered by a dense and uniform GLC coating. Contact angle of GLC coating was reduced to 27.2° when it was functionalized by H2O2 oxidation at 40 °C for 1 h. In vitro cytological test showed that the RGD peptides immobilized GLC coating had a significant improvement in biocompatibility. It was suggested that RGD peptides provided GLC coating with a bioactive surface to improve cell adhesion and proliferation on C/C composites.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China under Grant Nos. 51202194 and 51221001, the Programme of Introducing Talents of Discipline to Universities (“111” project of China) under Grant No. B08040.

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Authors and Affiliations

  1. State Key Laboratory of Solidification Processing, Northwestern Polytechnical University, Xi’an, 710072, China

    Sheng Cao, He-Jun Li, Ke-Zhi Li, Jin-Hua Lu & Lei-Lei Zhang

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  1. Sheng Cao
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  2. He-Jun Li
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  3. Ke-Zhi Li
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  4. Jin-Hua Lu
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  5. Lei-Lei Zhang
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Correspondence to He-Jun Li.

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Cao, S., Li, HJ., Li, KZ. et al. In Vitro Biocompatibility of MC3T3-E1 Osteoblast-like Cells on Arg-Gly-Asp Acid Peptides Immobilized Graphite-like Carbon Coating on Carbon/Carbon Composites. Acta Metall. Sin. (Engl. Lett.) 30, 558–566 (2017). https://doi.org/10.1007/s40195-017-0542-2

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  • DOI: https://doi.org/10.1007/s40195-017-0542-2

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