Update on Epidemiology and Treatment of MRSA Infections in Children
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In 2000–2010, the epidemiology of pediatric MRSA infections in the United States was transformed with an epidemic of CA-MRSA infections. We review the epidemiology of MRSA in the community and in the health care setting, including intensive care units, among infants and CF patients, and in households, as well as the impact that the CA-MRSA epidemic has had on hospitalization with MRSA infections. Risk factors for carriage, transmission, and initial and recurrent infection with MRSA are discussed. New studies on the treatment of pediatric MRSA infections and on the efficacy of MRSA decolonization are reviewed.
Staphylococcus aureus isolates, including methicillin-resistant S. aureus (MRSA) strains, are common causes of skin and soft tissue infections (SSTIs), bacteremia, osteomyelitis, and other invasive infections . MRSA strains almost universally carry the mecA gene, which confers resistance to nearly all β-lactam antibiotics. mecA is carried on a genetic element, the staphylococcal chromosome cassette mec (SCCmec). Until the late 1990s, MRSA infections were almost always associated with exposure to the health care setting. The epidemiology of MRSA became complex when, in the mid-1990s, novel MRSA strains were recognized that caused infections in previously healthy children [2, 3]. These are called community-associated (CA-) MRSA, in contrast to the healthcare-associated (HA-) MRSA that had been recognized from the early 1960s. Beginning in about 2001, an epidemic of CA-MRSA infections has occurred in North America, Taiwan, Korea, and Australia, and a lower prevalence of CA-MRSA infections has been documented simultaneously in Europe, Latin America, and other parts of Asia.
CA-MRSA strains vary geographically in their genetic backgrounds, although they usually carry relatively small SCCmec types IV, V, or VT in Taiwan, and nearly uniformly carry genes encoding the Panton–Valentine leukocidin (PVL), an otherwise unusual toxin that lyses human neutrophils. In contrast, HA-MRSA strains rarely carry the pvl toxin genes. HA-MRSA strains predominantly carry SCCmec type II in North America and type III in other regions of the world. While these genetic traits can distinguish CA- from HA-MRSA, this distinction does not correlate well with the setting of onset of a MRSA infection (i.e., community or health care). CA- and HA-MRSA strains have both been found commonly in the health care setting since 2004. Both also cause infections originating in the community . CA-MRSA strains tend to carry fewer non-β-lactam genetic resistance elements than HA-MRSA strains . Nevertheless, the distinction between CA- and HA-MRSA is often important for clinical studies.
In the U.S. in the early twenty-first century, there have been two predominant MRSA strain types defined by pulsed field gel electrophoresis (PFGE). USA100 [usually ST5 by multilocus sequence typing (MLST) with SCCmec type II and lacking pvl] is the predominant HA-MRSA strain type, and USA300 (usually ST8 with SCCmec type IV, and pvl+) is the predominant CA-MRSA type. USA300 has greater “fitness,” virulence and transmissibility  compared with genotypically defined HA-MRSA isolates.
In this review, we describe studies on MRSA epidemiology and therapy in children published after the recognition of CA-MRSA, focusing on recent studies from the U.S.
Maternal and Infant MRSA Colonization
Studies of rectovaginal or peripartum maternal MRSA colonization, 2006–2012
Year (s) of testing
Anatomic site tested (broth used)a
n with MRSA/n tested (%)
Genotypes identified (n or %)
New York City (Columbia U.)
Pregnant women 35–37 weeks gestation
Perirectal and lower vagina (Lim broth)
14/2,963 (0.47 %)
SCCmec type IV (12); SCCmec type V (1); other SCCmec type (1)
New York City (Columbia U.)
Pregnant women 35–37 weeks gestation
Perirectal and lower vagina (Lim broth)
18/2,921 (0.6 %)
USA300 (12); other (6)
New York City (Lutheran Med. Center)
Pregnant women ≥35 weeks
7/6,626 (0.1 %)
Pregnant women 35–37 weeks gestation
Rectovaginal culture (Lim broth)
26/250 (10.4 %)
SCCmec type IV (7); other type (19)
Pregnant women 35–37 weeks gestation
202/5,732 (3.5 %)
Pregnant women admitted to labor and delivery delivering within 24 h
Vaginal and nares culture (nb)
6/288 (2.1 %)
SCCmec type IV (4); SCCmec type II (2)
Pregnant women admitted for pre-term delivery
Vulva, rectovaginal, and nares culture (nb)
15/422 (3.6 %)
Pregnant women 35–37 weeks gestation
Rectovaginal (Lim broth)
26/1,488 (1.7 %)
Pregnant women admitted to labor and delivery
Vagina and nares (nb)
2/96 (2.1 %)
Pregnant women admitted to labor and delivery
Nares (PCR only)
39/2,254 (2 %)
Instead of intrapartum vertical transmission, two studies suggest that transmission of MRSA or other S. aureus post-partum from mothers to infants occurs. In 2007–2009 in Memphis, Tennessee, among 50 maternal–infant pairs studied up to 4 months post-partum who had concurrent MRSA colonization, 76 % had isolate pairs of the same pulsotype, and 30 % of the pairs both carried USA300, most identified only long after birth [17•]. Although the molecular epidemiology of MRSA differs in Europe, a study from a Greek hospital had similar results .
Infants are also at risk for MRSA colonization from non-maternal sources. In 2005–2007, among a convenience sample of healthy newborn infants in Washington State, 8/23 (35 %) had S. aureus isolated from stool, anterior nares, and/or periumbilical skin after hospital discharge at 2 weeks of age. Broth enrichment culture was not used. Two of these infants carried MRSA, but there was no MRSA nasal carriage among their parents . Thus, MRSA colonization in infants can arise from many sources in the community, and, at least most of the time, it is not likely to be derived from maternal rectovaginal colonization at birth.
Neonatal Intensive Care Unit (NICU) MRSA Epidemiology
S. aureus, including MRSA, has long been a common cause of serious infections in pediatric ICUs (PICUs) and NICUs. The epidemiology of MRSA in NICUs changed in 2000–2010 as CA-MRSA, USA300 in the U.S., became more common among vulnerable patients in this setting [20•, 21, 22]. Among very-low-birth-weight infants, S. aureus is an unusual cause of early systemic infection, but it is more common as a cause of late-onset systemic infection .
The emergence of USA300 in NICUs was documented in New York City and Boston. At a NICU in New York City with about 1,100 discharges per year in 2000–2008, during routine screening and 4 outbreak investigations, 8.6 % (115/1,336) of screened neonates were colonized with MRSA. Among 93 NICU patients with actual MRSA infections, USA100 predominated during 2001–2004. USA300 became the predominant MRSA pulsotype (10/12 typed isolates) during 2007 [20•]. In 2001–2009 at the Children’s Hospital of Boston, among 15,295 pediatric ICU patients, 3.2 % (495/15,295) carried MRSA in the nares, and the proportion of MRSA isolates increased that carried SCCmec IV (p = 0.03), and pvl (p = 0.003). No isolate was pvl+ in 2003, but 28.9 % were pvl+ in 2009 . At Case Western Reserve University, the incidence of MRSA infections in the NICU was higher in 2006–2008 compared with 2001–2005. USA300 predominated among available MRSA isolates in 2006–2008; there were none from 2001–2005 .
In a Boston NICU during 2000–2007, 1.3 % (102/7997) of the patients were MRSA carriers. The incidence of MRSA infections decreased from 1.79 per 1,000 patient-days in 2000 to 0.15 in 2005 with the introduction of weekly nasal and rectal surveillance cultures, but then increased to 1.26 in 2007. The antibiogram of MRSA isolates shifted from one suggesting dominance of HA-MRSA in 2000–2004 to one suggesting CA-MRSA in 2006–2007 .
At the University of Chicago Medical Center NICU in 2007–2008, 13/614 (2.1 %) infants on admission carried MRSA in the mouth, axilla or groin. Carriage on admission was not a strong predictor of MRSA infection during the first year of life . Thus, the epidemiology of MRSA infection in NICU patients is shifting and the predominant source of USA300 colonizing NICU patients is not defined; it may be staff or visitors.
Pediatric Intensive Care Unit (PICU) MRSA Epidemiology
Patients in PICUs in the United States have experienced a change in circulating MRSA strain types during the first decade of the twenty-first century. At the Johns Hopkins PICU in 2007–2008, 6 % (72/1674) of patients had MRSA colonization. Colonized patients were younger, more likely to be black and to have been hospitalized in the previous year than those without colonization. Of the MRSA isolates obtained, 61 % (40/66 tested) were USA300 or USA400, CA-MRSA strain types [26•]. Thus, as in NICUs, MRSA infections in PICUs may be predominantly caused by CA-MRSA strain types; MRSA epidemiology in the community may affect colonization trends in U.S. PICUs.
Trends in Pediatric MRSA-Associated Hospitalization and Infection Incidence
As early as 2003, young age was determined to be a risk factor for CA-MRSA infection compared with HA-MRSA , and newer evidence has continued to support this association. In a retrospective study in New York City in 1997–2006, age <18 years was a risk factor for CA-MRSA-related hospitalization (adjusted OR 1.29, p < 0.01) . In Orange County, California, in 2008–2009, hospitalized children with a MRSA infection were more likely than adults to be infected by a CA-MRSA strain type . In a meta-analysis, the percent of CA-MRSA among all MRSA infections in U.S. children plateaued at about 70 % in 1995, far earlier than among adults, for whom it was still rising in 2010 .
In the U.S. [30, 31, 32] and Canada , the epidemic of CA-MRSA was associated with a rapid increase in hospital stays for SSTIs among children in 1995–2010. Using the National Hospital Discharge Survey, a federal database that includes billing data from a sample of U.S. hospital stays, it was estimated in 1996–2006 that 616,375 children were hospitalized with a primary diagnosis of SSTI. The incidence increased from <1 in 1996 to 25.5 per 100,000 in 2006 .
In California, data from a state-wide hospital discharge database showed a rapid rise in population-based incidence of pediatric hospitalizations for S. aureus infections from 1985 to 2009. In 1985, 49/100,000 children were hospitalized for a S. aureus infection. With the emergence of CA-MRSA infections, the incidence increased by >50 %, peaked at 83/100,000 in 2006 and then decreased to 73/100,000 in 2009. This last decrease demonstrates a common trend in a number of epidemiologic studies in adults and children after 2006. The increase to 2006 was driven by MRSA SSTIs, infections in children 1–24 months of age and in black children of all ages. In 2000–2009, the incidence of MRSA infections in hospitalized children increased from 3 to 35/100,000 children per year. Hospitalizations for community-onset pediatric MRSA infections increased 27.7-fold in 2000–2006 [34••].
Studies based on laboratory data have similarly shown an increase in MRSA infections. A laboratory-based surveillance study at UCLA in 2001–2010 found that, among inpatient and outpatient pediatric SSTI cultures, 33.2 % (1,119/3,372) grew S. aureus, and 40.1 % (449/1,119) were MRSA, with a peak (49 %) in 2005. The percent of MRSA among outpatient S. aureus SSTIs changed little during 2004–2007 (annual mean, 47.8 %), prior to a slow decline in 2008–2010 . Thus, the epidemic of CA-MRSA infections had an impact on hospitalizations for children in the U.S., both as a cause of infections in the community and in the health care setting.
MRSA Colonization in Children: Geographic Variation and Risk Factors
Studies on MRSA Carriage in Healthy Children, 2009–May 2013
Year (s) of testing
Place of testing
Anatomic site tested (broth used)a
n with MRSA/n tested (%)
Genotypes identified (n or %)
North Carolina and Virginia
24 child care centers
15/163 (1.3 %)
ST5 (6); ST8/IV/pvl+ (5); ST1532 (1); ST231 (1); ST97/IV/pvl− (1); ST5slv (1)
Children at a child care facility at a medical center
Nares, axillae, inguinal, perirectal area, or oropharynx (b)
7/104 (6.7 %)
USA300 (4); USA100 (2); USA600 (1)
Seoul, South Korea
Healthy children in 7 day care centers, 1–6 years old
40/428 (9.3 %)
ST72/IV (23); ST72/II (6); ST1765/IV (4); ST1765/II (2); other (5)
2- to 5-year-old children at day care centers
Nares or nasopharynx (b)
28/2,211 (1.3 %)
10 STs: ST59 (9); ST45 (7); ST10 (4) other ST (8). 2 SCCmec types: SCCmec type IV (13); SCCmec type V (15)
Nasopharynx, tested 3 times over 12 months (unk)
11/333 (3 %)
7 spa types; 6 STs
Portugal, 3 regions
2006, 2007, 2009
Children 6 years old and younger
3/2,100 (< 1 %)
ST939/IVa/pvl−/t324 (1); ST72/IVc/pvl−/t148 (1); ST931/VI/pvl−/t008 (1)
San Antonio de Areco, Argentina
Nares, axillae, inguinal, or pharynx (nb)
14/316 (4.4 %)
All SCCmec type IV; 8 closely related by PFGE
Healthy children, 2–6 years old
5/104 (4.8 %)
SCCmec type IV (2); SCCmec type II (1); SCCmec type I (2)
Siem Reap, Cambodia
Healthy children at doctor’s visits
87/2,485 (3.5 %)
ST834 (80); ST121 (2); ST188 (3); ST45 (1); ST9 (1)
Healthy children, 1–6 years old at 27 day care centers
6/500 (4.1 %)
Negev region, Israel
Children 2–12 months old at any of 5 doctors’ visits
32/553 (5.7 %)
ST916 (1); ST30 (1); ST911 (1); ST228 (1); ST5 (1); CC913 (16); ST247 (1); ST45 (3); other (7)
Healthy children 2–60 months old at clinic visits
Nares or nasopharynx (unk)
473/6,057 (7.8 %)
12 PFGE types; ST59/VT/pvl+ (22.8 %); ST59/IV/pvl− (59.2 %); other (18 %)
Healthy children 14 years or younger
131/1,615 (8.1 %)
ST59 (111); ST338 (1); other ST (19)
Healthy children 14 years or younger
240/1,585 (15.1 %)
ST59 (209); ST338 (15); other ST (16)
The household is likely an important site of transmission among U.S. children and their close contacts, as suggested by studies in Minnesota  and Michigan , and a study in adults and children in Los Angeles and Chicago . Home visits were made to 236 children in Minnesota after convalescence from a CA-MRSA infection. Patients and their 712 household contacts underwent nasal cultures for MRSA colonization without broth enrichment. Totals of 13 % (29/236) of case patients and 12 % (82/712) of contacts carried MRSA. At least one contact in 25 % (58/236) of households carried MRSA. 76 % of colonizing MRSA isolates obtained from case-patients and 87 % from their contacts shared the PFGE pulsotype of the index infection isolate from that household. Risk factors for household contacts to be MRSA-colonized included assisting the case patient in bathing or sharing of lotions with the case patient. The risk of colonization was decreased among contacts who used antibacterial soap . The study was limited by lack of extranasal cultures given the frequently exclusive extranasal CA-MRSA colonization identified in other studies .
In Detroit, MRSA nasal carriage was more frequent in 2007–2008 among pediatric inpatients with CA-MRSA infection (23 %, 18/77) than among uninfected control children (3.9 %, 3/77) (p ≤ 0.001) admitted to the same hospital. Similarly, MRSA carriage was more common among household contacts of patients (58 %, 14/24) compared with household contacts of controls (6.9 %, 2/29) (p = 0.001). At least one household contact in the infection patients’ households had a similar PFGE pulsotype to the index patient in 10/14 households , suggesting that there is likely to be household transmission of CA-MRSA.
S. aureus carriage on an individual may be polyclonal. A study from Illinois of 125 children tested for nasal carriage of S. aureus prior to surgery demonstrated that 26 % (32/125) carried at least two S. aureus strain types as defined by multi-locus variable-number tandem-repeat fingerprinting [53•]. This suggests that decolonization in children may result in unintended consequences because some strain types of S. aureus are commensals and not major pathogens.
In the U.S., the carriage of closely related MRSA isolates by several household members suggests that the household is likely an important site of MRSA transmission. Given the complex strain type dynamics of S. aureus colonization in households  and on individuals [53•], further research is needed to determine how to control transmission in households and among children generally, assessing for unintended consequences of decolonization regimens or other interventions.
Risk Factors for CA-MRSA Infection
Risk factors for CA-MRSA infection have recently been examined in children, and included obesity , chronic skin disease, especially eczema , previous SSTI, a close contact with a recent SSTI [54, 55•], and recent use of antibiotics [54, 56]. Interventions to protect these high-risk children have not been widely studied.
Native Hawaiians and Pacific Islanders (NHPI) have a high risk for S. aureus infections. In a study of children on Maui, Hawaii attending clinics in 2008–2009, cases with a S. aureus SSTI [28/71 (39.4 %) had MRSA and 43/71 (60.6 %) had MSSA], were compared with age-matched controls without an SSTI. Risk factors for a S. aureus SSTI were having a household contact with a S. aureus SSTI, abrasions or wounds of the skin, and being overweight. Among NHPI, the latter two risk factors were significant as were antibiotic use within 6 months and a history of eczema or another chronic skin disorder . It is not known if these risk factors apply to children in other parts of the U.S.
In St. Louis, in a 6-month period in 2006–2007, among children tested for S. aureus nasal colonization, a self-reported SSTI occurred in 23 % (6/26) of those with MRSA colonization compared with 8 % (16/194) with MSSA and 7 % (34/474) with no S. aureus. Independent risk factors for SSTI included SSTI in a household contact during the 6-month study period [adjusted OR (aOR) 7.6 (95 % CI 3.6, 16.4)] and a history of SSTI in the index child in the year prior to enrollment [aOR 5.3 (95 % CI 2.2, 13.1)]. Curiously, MRSA nasal carriage was not significantly associated with SSTI risk [aOR 2.8 (95 % CI 0.9, 8.6)] [55•]. However, among 3,140 children admitted to the Johns Hopkins PICU, those without nasal carriage of MRSA at admission were less likely (1.4 %) to develop a MRSA infection during hospitalization or after discharge than those with colonization on admission (8.5 %) (RR 5.9, 95 % CI 3.4–10.1) . A follow-up study that included self-report and chart abstraction to identify MRSA infections among patients with MRSA colonization during (n = 128) or prior to (n = 40) the index PICU stay, 9.5 % had a MRSA infection in the year after hospital discharge . Further research on the importance of MRSA nasal colonization in healthy children as an independent risk factor for subsequent MRSA infection is needed. In addition, the reasons that very young children are at elevated risk for MRSA infections relative to older children requires further research. For example, it is possible that the use of diapers may increase the risk of CA-MRSA carriage and infection.
Cystic Fibrosis (CF), MRSA Carriage, and Disease Progression
Among CF patients, chronic lower respiratory colonization by S. aureus is common and has long been recognized. For this reason, surveillance of respiratory secretions for S. aureus and other pathogens is often routinely performed. In the U.S., the prevalence of MRSA carriage in respiratory secretions increased from 7.3 % in 2001 to 25.9 % in 2011 , although there has been variability at different centers.
Some evidence suggests that MRSA carriage, and perhaps pvl+ MRSA carriage, may increase the risk of more rapid progression of illness and death although the importance of these observations in patients with CF is controversial. At the St. Louis Children’s Hospital in 2001–2004, six pediatric CF patients carried pvl+ strains of MRSA. These six patients were at higher risk for hospital admission requiring intravenous antibiotics, for focal lung infiltrates, and for a more rapid decrease in FEV1 in the year following isolation of MRSA in the sputum than 34 CF patients colonized with MRSA that was not pvl+ . In contrast, a 2-center study showed that in 2004–2005, there was no difference in the likelihood of having a pvl+ MRSA isolate obtained from the respiratory secretions of a CF patient with a CF exacerbation (3/11) or from a routine surveillance culture (7/23) (p = 1.0) .
MRSA infection in patients who were 6–17 years of age in the Epidemiologic Study of Cystic Fibrosis during 2001 was associated with more airflow obstruction (FEV1 80.7 % predicted) compared with patients with MSSA infection (FEV1 89.4 % predicted) . Similarly, using the CF Foundation Patient Registry in 2003–2006, MRSA infection was a risk factor for lack of recovery of FEV1 after an exacerbation of CF . Even more striking, among 19,833 U.S. CF patients aged 6–45 years in 1996–2006 in the CF Foundation Patient Registry, persistent MRSA infection was associated with an increased risk of death (18.3 vs. 27.7 deaths per 1,000 patient-years). The adjusted hazard ratio of death in MRSA colonized patients was 1.27 (95 % CI 1.11–1.45). However, transient MRSA carriage that cleared within 1 year did not confer an increased risk of death [64••].
Not all studies have supported the association of MRSA colonization with a more rapid progression of disease in CF patients. In 120 CF patients with MRSA isolated from sputum in North Carolina in 2005–2007, 17 % of MRSA isolates were pvl+ and carried SCCmec type IV. FEV1 percent predicted did not differ among patients colonized with MRSA or with MSSA, even after adjusting for Pseudomonas colonization, age, and gender. While MRSA patients did require more pulmonary maintenance therapy (i.e., mucolytics, inhaled steroids, and chronic antimicrobial suppressive therapy), they did not require a greater number of antibiotic courses than did MSSA patients . In another cohort, MRSA-colonized patients had a faster decline in lung function even before acquisition of MRSA, suggesting that MRSA was not the cause of this decline but may have been a proxy marker .
Invasive and Complicated Infections in the General Pediatric Population
While the majority of MRSA infections in children are uncomplicated SSTIs, deep tissue and invasive infections were increasingly caused by MRSA in 1995–2010. Many studies of these infections unfortunately lack MRSA genotyping data.
The incidence of pediatric retropharyngeal abscesses at a Detroit hospital in 2004–2010 increased 2.8-fold compared with 1993–2003 (4.94 vs. 13.8 per 10,000 admissions). In the latter period, S. aureus was found in 38 % (25/66) of cultures with growth, compared with only 4.9 % (2/41) in 1993–2003. MRSA accounted for 64 % (16/25) of S. aureus isolates in 2004–2010, and none in 1993–2003 [67•]. At Emory University in Atlanta in 2004–2009, MRSA was isolated from 42 % (49/118) of community-onset deep neck space abscesses, while an additional 30 % (35/118) were caused by MSSA. Children <16 months of age were more likely than older children to have an abscess caused by MRSA or any S. aureus . In a review of 74 pediatric patients with 76 neck abscesses in Iowa in 2000–2010, 12 % grew MRSA and 31 % grew MSSA. There was only one MRSA abscess in 2000–2004 and seven in 2005–2010 . Similar results were obtained in a study from Toronto, Canada . Thus, with the emergence of USA300, S. aureus has become a more common cause of pediatric neck abscesses.
Necrotizing MRSA pneumonia complicating bacteremia or previous viral respiratory infection emerged as a sometimes fatal syndrome among previously healthy children in the era of CA-MRSA in the U.S. and elsewhere [4, 71, 72]. Among 838 U.S. pediatric patients with H1N1 influenza A infection admitted to PICUs in 2009–2010, 8.5 % (71/838) also had S. aureus pneumonia, of whom 48 % (34/71) had MRSA strains. Among 251 previously healthy children with influenza A infection, infection with a MRSA isolate <72 h after PICU admission was an independent risk factor for death . This study suggests that coinfection of influenza and MRSA, when occurring prior to ICU admission, results in severe pulmonary disease.
Cases of CA-MRSA and HA-MRSA necrotizing fasciitis in children with sometimes fatal outcome have been reported since 2009 in the U.S. [74, 75], and other countries [76, 77, 78, 79]. Bone and joint CA-MRSA infections have also been reported in previously healthy children in the U.S. [80, 81] and elsewhere , as have orbital abscesses [83, 84, 85, 86].
At some centers, CA-MRSA strains have come to predominate among causative isolates of MRSA causing pediatric catheter-associated bacteremia (CLABSI). For example, at Texas Children’s Hospital in 2001–2007, USA300 was isolated in 21/29 (72 %) of MRSA cases of CLABSI. In MRSA CLABSI cases, prolonged bacteremia was more common than in MSSA CLABSI, but there was no difference in the frequency of complications of endocarditis caused by USA300 or non-USA300 S. aureus .
Recurrent MRSA Infections
Frequent recurrence of MRSA infections, both invasive and non-invasive, has been noted in studies of children. As among adults, recurrence of MRSA infections in children is common, ranging from 9 to >50 % within a year. However, studies have used differing methods to detect recurrence, and few studies have assessed genetic data to determine if recurrent infections are due to the same MRSA strain types.
At the Akron Children’s Hospital ED, of 308 individuals <21 years of age (median age 7 years) treated for one or more skin abscesses (79 % of which were caused by MRSA) in January–June 2007, 14 % presented for at least one additional abscess during the study period. Also, among the 192 patients with available data, 43 % (82/192) had reported a history of a previous skin abscess .
A recurrent self-reported SSTI occurred within 1 year in >50 % of subjects in a study comparing two decolonization regimens in children who at baseline had a S. aureus SSTI (MSSA or MRSA) with subsequent S. aureus colonization [93•].
Although the predominant MRSA strain types differ in Taiwan, a study of recurrent infections in children there is noteworthy. In 2004–2006, of 66 paired isolates from an initial and a recurrent MRSA infection in individual children, 69.7 % (46/66) were genotypically indistinguishable by PFGE, and 19.7 % (13/66) differed by <4 bands. Distinct strain types were identified in an initial and recurrent infection in 7 (10.6 %) paired isolates. When an initial MRSA infection was caused by a pvl+ strain, recurrence with the same strain was more likely .
Further research is needed to assess the risk factors for recurrent MRSA infection in children, whether recurrence is more common with certain strain types of MRSA, the relationship of prolonged colonization and recurrence and the phenomenon of recurrent MRSA infections at different anatomic sites.
Treatment of Pediatric MRSA Infections
In 2011, the Infectious Disease Society of America published a treatment Guideline for MRSA infections in pediatric and adult infections. These Guidelines recommend the use of incision and drainage for all purulent SSTIs combined with antimicrobial drug therapy for lesions that are large or are accompanied by systemic symptoms. Drugs of choice for uncomplicated SSTIs should be chosen based on local trends in S. aureus epidemiology, but options include TMP-SMX [trimethoprim 4–6 (mg/kg)/dose, sulfamethoxazole 20–30 (mg/kg)/dose PO every 12 h], clindamycin [10–13 (mg/kg)/dose PO every 6–8 h, not to exceed 40 (mg/kg)/day], or, only for those greater than 8 years of age, either doxycycline [≤45 kg: 2 (mg/kg)/dose PO every 12 h; >45 kg: 100 mg BID] or minocycline [4 mg/kg PO × 1, then 2 (mg/kg)/dose PO every 12 h] [95••]. Here, we review recent reports assessing optimal therapies for MRSA infections in children.
In September 2006–May 2009 at Johns Hopkins University, 200 immunocompetent children with uncomplicated, purulent SSTIs treated as outpatients were randomized in a blinded study to receive cephalexin or clindamycin. There was no significant difference in clinical improvement 7 days later (94 vs. 97 % favorable outcomes), regardless of the size of the lesion. Among enrollees, 127/200 (64 %) underwent incision and drainage, 2/200 (1 %) had a needle aspiration, and 66/200 (33 %) had draining pus at presentation with no drainage procedure. Of the enrollees, 69 % had MRSA cultured. Three months later, recurrent SSTIs were self-reported or identified in medical records in 18 % of subjects (21 % of MRSA patients and 16 % of MSSA) .
Although the Hopkins study  suggests that antibiotic therapy may not be necessary for improved outcome of therapy for an uncomplicated CA-MRSA SSTI, effective antibiotic therapy may decrease the incidence of recurrent infections. In a double-blind randomized controlled trial among children (3 months to 18 years of age) in St. Louis, there was no significant difference in treatment failure in patients with uncomplicated fluctuant SSTIs after incision and drainage who were given TMP-SMX (4.1 %, 3/73) or placebo (5.3 %, 4/76). Eighty percent of recovered pathogens were MRSA and 9 % were MSSA. There was a higher rate of recurrent SSTI in the placebo than the TMP-SMX group within the first 10 days of follow-up (26.4 vs. 12.9 %), but not after 3 months (28.8 vs. 28.3 %) .
In a study of 6,407 children 0–17 years of age in 2004–2007 in Tennessee with Medicaid insurance coverage who had an SSTI, clindamycin had a smaller chance of treatment failure than either TMP-SMX (aOR 1.92, 95 % CI 1.49–2.47) or β-lactams (aOR 2.23, 95 % CI 1.71–2.90). Recurrence was also more likely with TMP-SMX (aHR 1.26, 95 % CI 1.06–1.49) or β-lactams (aHR 1.42, 95 % CI 1.19–1.69) compared with clindamycin therapy .
In summary, incision and drainage and wound care may be more important than an antibiotic active against S. aureus in pediatric uncomplicated SSTIs, although antimicrobial therapy, and perhaps clindamycin more than TMP-SMX, may decrease the incidence of recurrent infections.
It is not clear under what circumstances decolonization should be attempted in children who have MRSA infections. Also, the optimal regimen for decolonization is not established, and adverse effects of decolonization have not been fully explored, although two recent studies have added to our knowledge about decolonization.
In an open-label trial of pediatric (n = 193) and adult (n = 107) patients who had a S. aureus SSTI in 2007–2009 in St. Louis, intranasal mupirocin twice daily with a daily 15-min bleach bath for 5 days resulted in superior eradication of S. aureus after 4 months in the inguinal folds, axillae, and/or nares (71 %) compared with a 5-day regimen of either 4 % chlorhexidine baths plus mupirocin (54 %) or intranasal mupirocin alone (56 %). In each of these three intervention groups, and in a control group (48 % eradication), hygiene education was provided. In all groups combined, recurrent SSTIs were self-reported in 36 % of participants at 4 months [99••].
Despite the apparent decolonization of many participants, recurrent SSTIs were very common, suggesting that a single decolonization regimen is not likely to prevent recurrent disease. Weaknesses of this study included lack of measurement of adherence, lack of strain typing, and only self-report of recurrent infections.
No significant difference was found in eradication of S. aureus carriage among 183 children after treatment for a S. aureus SSTI who were randomized to a 5-day decolonization regimen of 4 % chlorhexidine baths plus intranasal mupirocin in the patients alone (50 % at 1 month; 54 % at 1 year) or to the same regimen applied to the entire household of the patient (51 % at 1 month; 66 % at 1 year). However, self-reported recurrent SSTIs at 12 months were more common in the patient-only decolonization group (72 %) than in the household decolonization group (52 %) (p = 0.02) [93•].
Thus, decolonization regimens applied to all individuals within a household may have superior efficacy in preventing recurrent SSTIs in a patient who had a S. aureus SSTI, but further studies powered to assess this outcome are necessary. Further studies are needed to determine which patients benefit from decolonization, and, in particular, which patients are at highest risk for recurrent disease. In addition, studies utilizing mupirocin or another antimicrobial for decolonization must assess the potential unintended consequence of emerging antibiotic resistance to these agents. Although decolonization may provide some benefit, control of S. aureus will not likely be achieved until effective S. aureus vaccines are developed and tested .
In 2000–2010, the molecular and clinical epidemiology of pediatric MRSA infections was transformed in the United States and Canada. This was driven by an epidemic of CA-MRSA SSTIs and the emergence of the USA300 pulsotype of MRSA in the community and in the health care setting. Additional research is needed in NICUs, among CF patients, in households, and in other community settings to determine risk factors for carriage, transmission, and infection with MRSA. Obesity should be the subject of further research given the high prevalence of both obesity and CA-MRSA in U.S. children. Continued surveillance of MRSA strains is necessary to anticipate changes in antimicrobial susceptibilities and trends in strain replacement. Regimens for the prevention of recurrent MRSA infection, such as decolonization, and the optimal choice of therapy of uncomplicated SSTIs must be clarified, in particular the risks and benefits of routine antibiotic therapy in addition to incision and drainage. A vaccine is likely needed to control MRSA among children.
Michael Z. David and Robert S. Daum declare that they have no conflicts of interest.
This article does not contain any studies with human or animal subjects performed by any of the authors.
Papers of particular interest, published recently, have been highlighted as: • Of importance •• Of major importance
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