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Synthesis of silver and gold nanoparticles by Sargassum muticum biomolecules and evaluation of their antioxidant activity and antibacterial properties

Abstract

Abstract

The present work reports on the antibacterial and antioxidant activity of gold and silver nanoparticles, synthesized by an extract of the brown macroalga Sargassum muticum (SM). These nanoparticles were synthesized and fully characterized by means of UV–Vis spectroscopy and electron microscopy. These techniques confirmed that spherical nanoparticles, with mean diameters of 10.4 ± 1.2 nm for Au@SM and 41.0 ± 5.7 nm for Ag@SM, were formed. To investigate the possible role of these biomolecules in this green synthetic process, Fourier-transformed infrared spectroscopy analysis was performed before and after the synthesis of nanoparticles. Furthermore, the carbohydrate composition was examined along with other variations observed after the synthesis of nanoparticles by size-exclusion chromatography. The study of the changes observed indicated that the polysaccharide fraction of the extract plays a role in the formation of the nanoparticles as well as in their stabilization. The in vitro antioxidant activity was analyzed by the determination of the reducing power, total content of phenolic compounds and the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) scavenging activity. Lastly, the antibacterial activity was tested against three types of bacteria with different cell wall composition. In particular, Ag@SM showed good inhibitory capacity on Gram+, especially on Staphylococcus aureus with a MIC of 3.38 µg/mL.

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Acknowledgements

This work was supported by the Xunta de Galicia (Ref.: ED431C 2017/46-GRC and ED431C 2018/54 -GRC).

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Correspondence to M. C. Rodríguez-Argüelles.

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González-Ballesteros, N., Rodríguez-Argüelles, M.C., Lastra-Valdor, M. et al. Synthesis of silver and gold nanoparticles by Sargassum muticum biomolecules and evaluation of their antioxidant activity and antibacterial properties. J Nanostruct Chem 10, 317–330 (2020). https://doi.org/10.1007/s40097-020-00352-y

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Keywords

  • Sargassum muticum
  • Gold nanoparticles
  • Silver nanoparticles
  • Antibacterial activity
  • Antioxidant activity