Microbial fuel cell with a nano-membrane and two expired medicinal drug-feeding cathode: a novel strategy
- 1.1k Downloads
In recent years, there has been developing interests in microbial fuel cell (MFC) technology as a hopeful approach to overcome worldwide energy crisis that will come into account by restricting fossil fuels consumption in the future. In addition, many drugs around the world are accessible to some populations easily and remain unused annually. This paper discusses bioelectricity generation with whey degradation in a dual chambered MFC in the presence of some expired medicinal drug-feeding cathodes. Consequently, a two-chamber MFC applied using Escherichia coli as biocatalyst, humic acid as electron mediator and Nafion 117 with a 5 nm in size as nano-membrane. The results showed that the open-circuit potential was 0.751 V at ambient condition. Stability of the voltage was exceeded 20 h. Acetaminophen codeine and Bismuth—as two expired medicinal drugs—applied as possible catholyte. In conclusion, Bismuth revealed more opportunity for power deriving in comparison with Acetaminophen. The best values were close to 2.9 × 10−5 W and 1.75 × 10−4 A, referring to power generation and current production, respectively.
KeywordsAcetaminophen codeine Bismuth Mediator Microbial fuel cell Whey
Biological oxygen demand
Chemical oxygen demand
Microbial fuel cell
Proton exchange membrane
Scanning electron microscope
By confronting environmental pollution issues and fossil fuels overconsumption, it is manifest for humanity to explore a utile, trusty, and clean energy system to replace the current energy production systems. A great volume of expired and outdated drugs as well as generated wastewater determined by chemical and biological oxygen demand (COD and BOD) concentrations [1, 2, 3, 4] are other important issue imposing lots of expense on governments around the world. In addition, Whey is a well-known product of cheese processing industry [5, 6] and could be used as suggested biochemical analyte for generation of bio-derived source electricity. The possibility of using MFC for power generation has studied before [7, 8, 9, 10]. Some researchers studied the principles of MFC especially in selecting microorganisms of interest , economical substrate , pilot-plant study for power production , modeling of biofilm constitution with engineering aspect , and role of electron acceptors in MFCs , respectively. The other groups verified the importance of this technology for power production [15, 16, 17, 18, 19, 20, 21]. Several kinds of mediators had applied in such system to augment the electron transfer rate, but they are generally expensive to be used in pilot or industrial scale program of MFC manufacture and showed toxicity to secreted biocatalysts for long-period operation . In addition, several microbial systems with various substrates and mediators have been reported [23, 24, 25]. To the best of our knowledge, this is the first survey presenting MFCs for generating electricity using expired drugs as electron shuttle. The main novelty was investigation and introduction of electricity harvesting from whey wastewater in the anode compartment, especially applying two expired medical drugs in cathode compartment of MFC as a new suggested trend and their functions as a light at the end of the tunnel in terms of power generation by MFC.
Materials and methods
Chemicals and materials
The microorganism growth monitoring was accomplished by spectrophotometer (Unico, USA) established as optical density. SEM (Philips XL30 scanning microscope, Philips, The Netherlands) employed to determine the shape and surface morphology of electrode in the present MFC. The graphite was coated with gold before the SEM measurement with the selected magnification of 5000.
Arrangement of medium and inoculum
The medium is comprised of glucose, yeast extract, ammonium chloride, and peptone in grouping of 10, 1.0, 0.5, and 1.0 g/l, respectively. It was disinfected by autoclaving at 121 °C, 15 psig for 20 min. Initially, the pH of medium, inoculum, and other parameters had adjusted in 7.0. Thereafter, it was heated at 30 °C. The E. coli had completely developed for 24 h in 100 ml flux with no additional shaking. The sampling was done in every 4.0 h to investigate the substrate utilization on reduced sugar content by aforementioned technique .
Anodes and nano-membrane pretreatment
To keep up layer for good conductivity, the two compartments preserved in double-distilled water when the bioreactor was not being applied . The anode compartment had introduced to serial pretreatment, which has been engrossing 100% ethanol for 45 min and in 1.0 M HCl for 1.0 h. After every usage, cathodes were immersed in 1.0 M HCl, 1.0 M NaOH, for 1.0 h, independently to clear any metallic and normal contamination by exposing indistilled water before use, respectively . Nafion membrane was exposed to serial treatment to remove contaminations which include washing the film for 1.0 h, immersion in 3.0% H2O2, following washing with double-distilled water, re-immersing in acidic solution and eventually re-exposing to distilled water, respectively.
Depiction of the data procurement framework
Simple computerized information procurement had produced to record sampling information in every 6 s. The framework records measurements for variable resistances which were imposed to the MFC. By dividing the obtained voltage from characterized resistance, the output current recorded. Then, the system provides power calculation by multiplication of voltage and current. In addition, the microcontroller sends the essential information to a PC by analogue–digital converter. Moreover, unique capacity of MATLAB programming (7.4, 2007a) was utilized to store and synchronically show the received information.
All facts are presented in this text as mean result ± SD (standard deviation). Statistical analysis were assessed applying the t test and considered significant at P < 0.05 level. All figures shown in this article have been received from three unbiased experiments with similar results.
Results and discussion
The mediator in MFC was the main factor in increasing electron transfer interestingly achieves more power production and cell current efficiency. The drawbacks of MFC were mentioned in the previous scientific papers . One of the expressed suggestions for overcoming proposed issue is identification of new mediators. Figures 3, 4 show power generation in the presence of two aforementioned expired medicinal drugs as hygienic organization wastes. The maximum power and current production in the presence of Acetaminophen and Bismuth as novel cathodic mediators was 2.55 × 10−5 W, 1.29 × 10−5 A, 2.9 × 10−5 W and 1.75 × 10−4 A, respectively. The maximum value of Voc is attributed to the presence of expired drugs as electron mediators. Humic acid acts as electron acceptor in the anode compartment and two expired medicines act as electron shuttle in the cathode chamber. These outputs are comparable to other researchers’ findings [15, 16], especially in a case without using nanomaterial except the selected membrane. In this work, a novel approach to bioelectricity generation and new limited medicinal waste management opportunity as a novelty was introduced and developed. To best of our knowledge, this is the first attitude as a light at the end of the tunnel upon applying and developing expired drugs as mediator in cathode compartment of a bioreactor like as MFC. Although the obtained quantities based on power and generation are not in high level, but the results open a new horizon for related industries and compartments to investigate more in such a new introduced concept.
Characterization of graphite electrode
COD and BOD removal efficiencies
Whey utilized as selected analyte in the MFC. The performance of MFC investigated using two expired medicinal drugs so-called Acetaminophen codeine and Bismuth implementing as mediatory agents in cathode compartment. The result of this study indicated that humic acid served as suitable mediator. Implemented Acetaminophen as expired drug was unsuitable choice as cathodic electron acceptor, but Bismuth opened a new horizon toward working on other expired therapeutic drugs in cathode compartment for ideal performance. The most interesting finding was that the produced power in the presence of Acetaminophen codeine was lower than OCV in the absence of catholyte. The reason for this is not clear, but it may have something to do with relative competent groups for electricity exists in Acetaminophen codeine. The maximum power and current was 2.87 × 10−5 W and 1.75 × 10−4 A, respectively, although further studies will be need on many aspects of this technology before these approaches could be commercialized successfully, but these results provided a new horizon to produce bioelectricity in a world where the energy crisis threatens its inhabitants. The future program will be focused on using different class of antibiotics and nano-materials to develop new introduced horizon.
The food and drug control reference laboratories and the water safety research center due to their laboratory facilities support in this work are acknowledged.
Compliance with ethical standards
Conflict of interest
The authors have declared no conflict of interest.
- 1.Akbari-adergani, B., Attaran, A.M., Veiskarami, M.: Optimization of parameters in concentration technology for treatment and reduction of organic load in distillery’s vinasse. J. Tolooebehdasht. 4, 21–31 (2013)Google Scholar
- 2.Akbari-adergani, B., Attaran, A.M., Taghimolla, Z., Shoeibi, Sh: Modification of designing system in chemical oxygen demand test with silver nano-particles for determination of organic load in effluent of pharmaceutical industries. J. Tolooebehdasht 3, 69–82 (2012)Google Scholar
- 7.Lini, K.M.: Microbial fuel cells: a promising tool for power generation. Int. J. Sci. Res. 4(7), 2530–2532. ISSN 2319-7064 (2013)Google Scholar
- 8.Bavasso, I., Di Palma, L., Petrucci, E.: Treatment of wastewater in H-Type MFC with protonic exchange membrane: experimental study of organic carbon and ammonium reduction with electrochemical characterization. Chem. Eng. Trans. 47, 1–6 (2016)Google Scholar
- 9.Vinicius, F.P., Sidney, A.N., Adalgisa, R.A., Valeria, R.: Energy generation in a microbial fuel cell using anaerobic sludge from a wastewater treatment plant. Sci. Agric. 7(35), 424–428 (2015)Google Scholar
- 10.Weihua, H., Xiaoyuan, Z., Jia, L., Xiuping, Z., Yujie, F., Bruce, E.L.: Microbial fuel cells with an integrated spacer and separate anode and cathode modules. Environ. Sci. Water Res. Technol. 2, 186–195 (2015)Google Scholar
- 11.Mao, L., Verwoerd, W.S.: Selection of organisms for systems biology study of microbial electricity generation: a review. Int. J. Energy Environ. Eng. 4(17), 1–18 (2013)Google Scholar
- 12.Sirinutsomboon, : Modeling of a membrane less single-chamber microbial fuel cell with molasses as an energy source. Int. J. Energy Environ. Eng. 5(93), 1–9 (2014)Google Scholar
- 15.Entchev, E., Yang, L., Chorab, M., Rosato, A., Sibilio, S.: (2016) Energy, economic and environmental performance simulation of a hybrid renewable microgeneration system with neural network predictive control. Alex. Eng. J. 1–19. https://doi.org/10.1016/j.aej.2016.09.001
- 18.Olga, T., Lihong, L., Aijie, W.: Electricity generation by Enterobacter sp. of single-chamber microbial fuel cells at different temperatures. J. Clean Energy Technol. 4(1), 36–42 (2016)Google Scholar
- 21.Sarika, A.L., Vibhavari, D.P., Deelip, B.P.: Role of mediators in microbial fuel cell for generation of electricity and waste water treatment. Int. J. Chem. Sci. Appl. 6(1), 6–11 (2015)Google Scholar
- 22.Swamini, P., Kris, S., Abhilash, B., Froila, D., Yash, M., Vikram, S., Ritika, R., Joice, M., Radhika, B., Aruna, K.: Bioremediation of dye effluent waste through an optimized microbial fuel cell. Int. J. Adv. Res. Biol. Sci. 3, 214–226 (2016)Google Scholar
- 23.Venkatesh, C., Pradeep, V.: Microbial fuel cell: a green approach for the utilization of waste for the generation of bioelectricity. Bioresour. Bioprocess. 3(38), 1–14 (2016)Google Scholar
- 24.Thomas, L.C., Chamberlin, G.J., Ltd, Tintometer: Colorimetric chemical analytical methods, p. 31. Tintometer Ltd, Salisbury (1980)Google Scholar
- 28.Mathuriya, A.S., Sharma, V.N.: Bioelectricity production from various wastewaters through microbial fuel cell technology. J. Biochem. Technol. 2, 133–137 (2010)Google Scholar
- 30.Ucar, D., Yifeng Zhang, Y., Angelidaki, I.: An overview of electron acceptors in microbial fuel cells. Front. Microbiol. 8(643), 1–14 (2017)Google Scholar
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.