In this work, a new sensitive enzyme-based electrode for electrochemical cholesterol biosensor was fabricated based on a nanocomposite of Au nanoparticles, ZnO nanoparticles and multi-wall carbon nanotubes (Au/ZnO/MWCNTs). The nanocomposite was prepared by sol–gel method and deposited on FTO substrate by dip coating, followed by cholesterol oxidase (ChOx) enzyme immobilized (ChOx/Au/ZnO/MWCNTs). Structural properties and morphology of the nanocomposite have been studied using X-ray diffraction (XRD) and Field emission scanning electron microscopy (FESEM). The sample was subjected to Fourier transform infrared spectroscopy (FTIR) to determine functional groups. Electrochemical behavior of the electrode was studied by cyclic voltammetry (CV) and differential pulse voltammetry (DPV) techniques as a function of cholesterol concentration. Electrochemical impedance spectroscopy (EIS) was also considered to study of surface modified electrodes. The ChOx/Au/ZnO/MWCNTs electrode has been found to have enhanced electron transfer and display excellent analytical linear performances. The fabricated electrode exhibited low detection limit (0.1 μM), high sensitivity (25.89 μA/μM) evaluated from DPV data in the detection range of 0.1–100 µM and high selectivity in the determination of cholesterol over glucose and uric acid. The application of the ChOx/Au/ZnO/MWCNTs electrode in detection of cholesterol in human serum was also confirmed.
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Baynes, J.W., Dominiczak, M.: Medical Biochemistry, 2nd edn. Elsevier, Amsterdam (2005)
Li, L.H., Dutkiewicz, E.P., Huang, Y.C., Zhou, H.B., Hsu, C.C.: Analytical methods for cholesterol quantification. J. Food Drug Anal. (2019). https://doi.org/10.1016/j.jfda.2018.09.001
Li, R., Xiong, C., Xiao, Z., Ling, L.: Colorimetric detection of cholesterol with G-quadruplex-based DNAzymes and ABTS2-. Anal. Chim. Acta (2012). https://doi.org/10.1016/j.aca.2012.02.015
Dervisevic, M., Çevik, E., Şenel, M., Nergiz, C., Abasiyanik, M.F.: Amperometric cholesterol biosensor based on reconstituted cholesterol oxidase on boronic acid functional conducting polymers. J. Electroanal. Chem. (2016). https://doi.org/10.1016/j.jelechem.2016.06.033
Molaei, R., Sabzi, R.E., Farhadi, K., Kheiri, F., Forough, M.: Amperometric biosensor for cholesterol based on novel nanocomposite array gold nanoparticles/acetone-extracted propolis/multiwall carbon nanotubes/gold. Micro Nano Lett. (2014). https://doi.org/10.1049/mnl.2013.0664
Saxena, U., BikasDas, A.: Nanomaterials towards fabrication of cholesterol biosensors: key roles and design approaches. Biosens. Bioelectron. (2016). https://doi.org/10.1016/j.bios.2015.08.042
Devi, S., Rodrigues, F., Meenakshi, S., Pandian, K., Perumal, P.: Carbon nanotube based amperometric biosensor for the quantitative detection of cholesterol. Biotechnol. Biochem. (2017). https://doi.org/10.9790/264X-03021020
Cai, X., Gao, X., Wang, L., Wu, Q., Lin, X.: A layer-by-layer assembled and carbon nanotubes/gold nanoparticles-based bienzyme biosensor for cholesterol detection. Chemi. Sens Actuators B (2013). https://doi.org/10.1016/j.snb.2013.02.050
Lin, X., Ni, Y., Kokot, S.: Electrochemical cholesterol sensor based on cholesterol oxidase and MoS2-AuNPs modified glassy carbon electrode. Sens Actuators B: Chem. (2016). https://doi.org/10.1016/j.snb.2016.04.019
Mokwebo, K.V., Oluwafemi, O.S., Arotiba, O.A.: An electrochemical cholesterol biosensor based on a CdTe/CdSe/ZnSe quantum dots-poly (propylene imine) dendrimer nanocomposite immobilisation layer. Sensors (2018). https://doi.org/10.3390/s18103368
Ghosh, S., Ahmad, R., Khare, S.K.: Immobilization of cholesterol oxidase: an overview. Open Biotechnol. J. (2018). https://doi.org/10.2174/1874070701812010176
Zhu, Z.: An overview of carbon nanotubes and graphene for biosensing applications. Nano-Micro Lett. 9, 1–24 (2017). https://doi.org/10.1007/s40820-017-0128-6
Sireesha, M., Jagadeesh Babu, V., Kranthi Kiran, A.S., Ramakrishna, S.: A review on carbon nanotubes in biosensor devices and their applications in medicine. Nanocomposites (2018). https://doi.org/10.1080/20550324.2018.1478765
Baldo, S., Buccheri, S., Ballo, A., Camarda, M., La Magna, A., Castagna, M.E., Romano, A., Iannazzo, D., Raimondo, F.D., Neri, G., Scalese, S.: Carbon nanotube-based sensing devices for human arginase-1 detection. Sens Bio-Sens. Res (2016). https://doi.org/10.1016/j.sbsr.2015.11.011
Gupta, V.K., Norouzi, P., Ganjali, H., Faridbod, F., Ganjali, M.R.: Flow injection analysis of cholesterol using FFT admittance voltammetric biosensor based on MWCNT–ZnO nanoparticles. Electrochim. Acta (2013). https://doi.org/10.1016/j.electacta.2013.03.118
Zhang, C., Wang, G., Ji, Y., Liu, M., Feng, Y., Zhang, Z., Fang, B.: Enhancement in analytical hydrazine based on gold nanoparticles deposited on ZnO-MWCNTs films. Sens Actuators B: Chem. (2010). https://doi.org/10.1016/j.snb.2010.07.007
Mehmood, S., Carlino, E., Bhatti, A.S.: Role of Au(NPs) in the enhanced response of Au(NPs)-decorated MWCNT electrochemical biosensor. Int. J. Nanomed. (2018). https://doi.org/10.2147/IJN.S155388
Gahlaut, A., Hooda, V., Dhull, V., Hooda, V.: Recent approaches to ameliorate selectivity and sensitivity of enzyme based cholesterol biosensors: a review. Artif. Cells Nanomed. Biotechnol. (2017). https://doi.org/10.1080/21691401.2017.1337028
Solanki, P.R., Kaushik, A., Ansari, A.A., Malhotra, B.D.: Nanostructured zinc oxide platform for cholesterol sensor. Appl. Phys. Lett. (2009). https://doi.org/10.1063/1.3111429
Zhao, Z., Lei, W., Zhang, X., Wang, B., Jiang, H.: ZnO-based amperometric enzyme biosensors. Sensors (Basel) (2010). https://doi.org/10.3390/s100201216
Paliwal, A., Gaur, R., Sharma, A., Tomar, M., Gupta, Vinay: Sensitive optical biosensor based on surface plasmon resonance using ZnO/Au bilayered structure. Optik (2016). https://doi.org/10.1016/j.ijleo.2016.05.103
Zang, Y., Fan, J., Yun, J., Xue, H., Pang, H.: Current advances in semiconductor nanomaterial-based photoelectrochemical biosensing. Eur. J. Chem. (2018). https://doi.org/10.1002/chem.201801358
Cullity, B.D., Stock, S.R.: Elements of X-ray diffraction, vol. 3. Prentice Hall, New-Jersey (2001)
Ahmed, D.S., Haider, A.J., Mohammad, M.R.: Comparesion of functionalization of multi walled carbon nanotubes treated by oil olive and nitric acid andtheircharacterization. Energy Procedia (2013). https://doi.org/10.1016/j.egypro.2013.07.126
Finnie, K.S., Cassidy, D.J., Bartlett, J.R., Woolfrey, J.L.: IR spectroscopy of surface water and hydroxyl species on nanocrystalline TiO2 films. Langmuir (2001). https://doi.org/10.1021/la0009240
Vafaee, M., Ghamsari, M.S.: Preparation and characterization of ZnO nanoparticles by a novel sol–gel route. Mater. Lett. (2007). https://doi.org/10.1016/j.matlet.2006.11.089
Alagappan, M., Immanuel, S., Sivasubramanian, R., Kandaswamy, A.: Development of cholesterol biosensor using Au nanoparticles decorated f-MWCNT covered with polypyrrole network. Arab. J. Chem. (2018). https://doi.org/10.1016/j.arabjc.2018.02.018
Azhar, M., Nawaz, H., Majdinasab, M., Latif, U., Nasir, M., Gokce, G., WaqasAnwar, M., Hayat, A.: Development of a disposable electrochemical sensor for detection of cholesterol using differential pulse voltammetry. J. Pharm. Biomed. Anal. (2018). https://doi.org/10.1016/j.jpba.2018.07.005
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Ghanei Agh Kaariz, D., Darabi, E. & Elahi, S.M. Fabrication of Au/ZnO/MWCNTs electrode and its characterization for electrochemical cholesterol biosensor. J Theor Appl Phys 14, 339–348 (2020). https://doi.org/10.1007/s40094-020-00390-5
- Electrochemical biosensor
- Cholesterol oxidase
- Au nanoparticles
- ZnO nanoparticles
- Multi-wall carbon nanotubes
- Au/ZnO/MWCNTs nanocomposite