Skip to main content
SpringerLink
Log in

Transcriptional responses following seed priming with cold plasma and electromagnetic field in Salvia nemorosa L.

  • Research
  • Published:
Journal of Theoretical and Applied Physics

Abstract

This study was conducted to monitor the plant responses to seed priming with electromagnetic fields (0, 4, or 6 mT) or cold plasma (0, 80, or 100 s) in Salvia nemorosa. The cold plasma or electromagnetic field treatments significantly increased shoot fresh weight (49%), root fresh weight (41%), and root length (56%). The results highlighted that seed priming with cold plasma or the electromagnetic field is an effective method to modify seedling growth. The electromagnetic field and plasma treatments upregulated the AREB1 gene (mean = 3.9-fold). Except for the electromagnetic field of 4mT, the other treatments stimulated expressions of the WRKY1 gene by an average of 6.7-fold relative to the control. The cold plasma or electromagnetic field also induced the expression of cinnamoyl-CoA reductases (CCR2) gene (mean = fourfold). These treatments also changed the expression of the rosmarinic acid synthase by an average of sixfold. These findings may improve our knowledge of plant reactions to cold plasma and electromagnetic field for possible functions in seed technology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  1. Bahadori, M.B., Eskandani, M., Mieri, M.De, Hamburger, M., Nazemiyeh, H.: Anti-proliferative activity-guided isolation of clerodermic acid from Salvia nemorosa L.: geno/cytotoxicity and hypoxia-mediated mechanism of action. Food Chem. Toxicol. 120, 155–163 (2018)

    Article  Google Scholar 

  2. Kuzma, L., Wysokinska, H.: Production of secondary metabolites in shoots of Salvia nemorosa L. cultured in vitro. Biotechnologia 4, 154–159 (2003)

    Google Scholar 

  3. Tien, P., Wang, S.R.: The influences of extremely low frequency AC magnetic fields at 60 Hz on mung beans growth. J. Am. Sci. 5, 49 (2009)

    Google Scholar 

  4. Safari, N., Iranbakhsh, A., Ardebili, Z.O.: Non-thermal plasma modified growth and differentiation process of Capsicum annuum PP805 Godiva in in vitro conditions. Plasma Sci. Technol 19, 055501 (2017)

    Article  ADS  Google Scholar 

  5. Iranbakhsh, A., Ghoranneviss, M., Ardebili, Z.O., Ardebili, N.O., Tackallou, S.H., Nikmaram, H.: Non-thermal plasma modified growth and physiology in Triticum aestivum via generated signaling molecules and UV radiation. Biol. Plant. 61, 702–708 (2017)

    Article  Google Scholar 

  6. Babajani, A., Iranbakhsh, A., Ardebili, Z.O., Eslami, B.: Seed priming with non-thermal plasma modified plant reactions to selenium or zinc oxide nanoparticles: cold plasma as a novel emerging tool for plant science. Plasma Chem. Plasma Process. 39, 21–34 (2019). https://doi.org/10.1007/s11090-018-9934-y

    Article  Google Scholar 

  7. Šerá, B., Zahoranová, A., Bujdáková, H.: Šerý M Disinfection from pine seeds contaminated with Fusarium circinatum Nirenberg & O’Donnell using non-thermal plasma treatment. Rom. Rep. Phys. 71, 701 (2019)

    Google Scholar 

  8. Moghanloo, M., Iranbakhsh, A., Ebadi, M., Satari, T.N., Ardebili, Z.O.: Seed priming with cold plasma and supplementation of culture medium with silicon nanoparticle modified growth, physiology, and anatomy in Astragalus fridae as an endangered species. Acta Physiol. Plant. 41, 54 (2019)

    Article  Google Scholar 

  9. Iranbakhsh, A., Ardebili, N.O., Ardebili, Z.O., Shafaati, M., Ghoranneviss, M.: Non-thermal plasma induced expression of heat shock factor A4A and improved wheat (Triticum aestivum L.) growth and resistance against salt stress. Plasma Chem. Plasma Process. 38, 29–44 (2018)

    Article  Google Scholar 

  10. Seddighinia, F.S., Iranbakhsh, A., Ardebili, Z.O., Satari, T.N., Soleimanpour, S.: Seed priming with cold plasma and multi-walled carbon nanotubes modified growth, tissue differentiation, anatomy, and yield in bitter melon (Momordica charantia). J. Plant Growth Regul. 39, 87–98 (2020). https://doi.org/10.1007/s00344-019-09965-2

    Article  Google Scholar 

  11. Gao, X., Zhang, A., Héroux, P., Sand, W., Sun, Z., Zhan, J., Wang, C., Hao, S., Li, Z., Guo, Y.: Effect of Dielectric Barrier Discharge Cold Plasma on Pea Seed Growth. Agric. Food Chem. 67, 10813–10822 (2019)

    Article  Google Scholar 

  12. Sheteiwy, M.S., An, J., Yin, M., Jia, X., Guan, Y., He, F., Hu, J.: Cold plasma treatment and exogenous salicylic acid priming enhances salinity tolerance of Oryza sativa seedlings. Protoplasma 256, 79–99 (2019)

    Article  Google Scholar 

  13. Moghanloo, M., Iranbakhsh, A., Ebadi, M., Ardebili, Z.O.: Differential physiology and expression of phenylalanine ammonia lyase (PAL) and universal stress protein (USP) in the endangered species Astragalus fridae following seed priming with cold plasma and manipulation of culture medium with silica nanoparticles. Biotech 9, 288 (2019)

    Google Scholar 

  14. Iranbakhsh, A., Ardebili, Z.O., Ardebili, N.O., Ghoranneviss, M., Safari, N.: Cold plasma relieved toxicity signs of nano zinc oxide in Capsicum annuum cayenne via modifying growth, differentiation, and physiology. Acta Physiol. Plant. 40, 154 (2018)

    Article  Google Scholar 

  15. Mildažienė, V., Aleknavičiūtė, V., Žūkienė, R., Paužaitė, G., Naučienė, Z., Filatova, I., Lyushkevich, V., Haimi, P., Tamošiūnė, I., Baniulis, D.: Treatment of common sunflower (Helianthus annus L.) seeds with radio-frequency electromagnetic field and cold plasma induces changes in seed phytohormone balance, seedling development and leaf protein expression. Sci. Rep. 9, 6437 (2019)

    Article  ADS  Google Scholar 

  16. Vashisth, A., Nagarajan, S.: Effect on germination and early growth characteristics in sunflower (Helianthus annuus) seeds exposed to static magnetic field. J. Plant Physiol. 167, 149–156 (2010)

    Article  Google Scholar 

  17. Sotoodehnia-Korani, S., Iranbakhsh, A., Ebadi, M., Majd, A., Ardebili, Z.O.: Selenium nanoparticles induced variations in growth, morphology, anatomy, biochemistry, gene expression, and epigenetic DNA methylation in Capsicum annuum; an in vitro study. Environ. Pollut. 265, 114727 (2020). https://doi.org/10.1016/j.envpol.2020.114727

    Article  Google Scholar 

  18. Yan, X., Liu, J., Kim, H., Liu, B., Huang, X., Yang, Z., Lin, Y.C., Chen, H., Yang, V., Wang, J.P., Muddiman, D.C.: CAD 1 and CCR 2 protein complex formation in monolignol biosynthesis in Populus trichocarpa. New Phytol. 222, 244–260 (2019)

    Article  Google Scholar 

  19. Liu, B., Honnorat, B., Yang, H., Arancibia, J., Rajjou, L., Rousseau, A.: Non-thermal DBD plasma array on seed germination of different plant species. J. Phys. D Appl. Phys. 52, 025401 (2018)

    Article  ADS  Google Scholar 

  20. Radhakrishnan, R., Kumari, B.D.: Pulsed magnetic field: a contemporary approach offers to enhance plant growth and yield of soybean. Plant Physiol. Biochem. 51, 139–144 (2012)

    Article  Google Scholar 

  21. Abedi, S., Iranbakhsh, A., Ardebili, Z.O., Ebadi, M.: Seed priming with cold plasma improved early growth, flowering, and protection of Cichorium intybus against selenium nanoparticle. J. Theor. Appl. Phys. 14(2), 113–120 (2020)

    Article  ADS  Google Scholar 

  22. Iranbakhsh, A., Ardebili, Z.O., Molaei, H., Ardebili, N.O., Amini, M.: Cold plasma up-regulated expressions of WRKY1 transcription factor and genes involved in biosynthesis of cannabinoids in Hemp (Cannabis sativa L.). Plasma Chem. Plasma Process. 40, 527–537 (2020)

    Article  Google Scholar 

  23. Rajaee Behbahani, S., Iranbakhsh, A., Ebadi, M., Majd, A., Ardebili, Z.O.: Red elemental selenium nanoparticles mediated substantial variations in growth, tissue differentiation, metabolism, gene transcription, epigenetic cytosine DNA methylation, and callogenesis in bittermelon (Momordica charantia); an in vitro experiment. PLoS One 15(7), e0235556 (2020). https://doi.org/10.1371/journal.pone.0235556

    Article  Google Scholar 

  24. Ling, L., Jiangang, L., Minchong, S., Chunlei, Z., Yuanhua, D.: Cold plasma treatment enhances oilseed rape seed germination under drought stress. Sci. Rep. 5, 13033 (2015)

    Article  ADS  Google Scholar 

  25. Srivastava, S., Vishwakarma, R.K., Arafat, Y.A., Gupta, S.K., Khan, B.M.: Abiotic stress induces change in Cinnamoyl CoA Reductase (CCR) protein abundance and lignin deposition in developing seedlings of Leucaena leucocephala. Physiol. Mol. Biol. Plant. 21, 197–205 (2015)

    Article  Google Scholar 

  26. So, H.A., Chung, E.S., Cho, C.W., Kim, K.Y., Lee, J.H.: Molecular cloning and characterization of soybean cinnamoyl CoA reductase induced by abiotic stresses. Plant Pathol. J. 26, 380–385 (2010)

    Article  Google Scholar 

  27. Zhang, X., Liu, D., Zhou, R., Song, Y., Sun, Y., Zhang, Q., Niu, J., Fan, H., Yang, S.Z.: Atmospheric cold plasma jet for plant disease treatment. Appl. Phys. Lett. 104, 043702 (2014)

    Article  ADS  Google Scholar 

  28. Babajani, A., Iranbakhsh, A., Ardebili, Z.O., Eslami, B.: Differential growth, nutrition, physiology, and gene expression in Melissa officinalis mediated by zinc oxide and elemental selenium nanoparticles. Environ. Sci. Pollut. R. 26, 24430–24444 (2019)

    Article  Google Scholar 

  29. Ghasempour, M., Iranbakhsh, A., Ebadi, M., Ardebili, Z.O.: Seed priming with cold plasma improved seedling performance, secondary metabolism, and expression of deacetylvindoline O-acetyltransferase gene in Catharanthus roseus. Contrib. Plasma Phys. 60, e201900159 (2020). https://doi.org/10.1002/ctpp.201900159

    Article  Google Scholar 

  30. Döring, A.S., Pellegrini, E., Della Batola, M., Nali, C., Lorenzini, G., Petersen, M.: How do background ozone concentrations affect the biosynthesis of rosmarinic acid in Melissa officinalis? J. Plant Physiol. 171, 35–41 (2014)

    Article  Google Scholar 

Download references

Acknowledgements

This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

Author information

Authors and Affiliations

  1. Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Maryam Ghaemi & Alireza Iranbakhsh

  2. Department of Biology, Faculty of Biological Sciences, North-Tehran Branch, Islamic Azad University, Tehran, Iran

    Ahmad Majd

Authors
  1. Maryam Ghaemi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ahmad Majd
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Alireza Iranbakhsh
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Alireza Iranbakhsh.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ghaemi, M., Majd, A. & Iranbakhsh, A. Transcriptional responses following seed priming with cold plasma and electromagnetic field in Salvia nemorosa L.. J Theor Appl Phys 14, 323–328 (2020). https://doi.org/10.1007/s40094-020-00387-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40094-020-00387-0

Keywords

Search

Navigation