The need to monitor the presence of cyanide (CN−) in water is necessary to minimize the risks to aquatic ecosystems and human health. In this paper, a paper-based analytical device (PAD) was fabricated by immobilizing silver nanoparticles (AgNPs) on filter paper (FP) for the semi-quantitative colorimetric detection of CN− in water. The average diameter of the synthesized AgNPs was estimated to be around 26.23 ± 8.37 nm, with a characteristic optical absorption peak around 420 nm. Scanning electron microscopy and energy-dispersive X-ray spectroscopy results confirmed the successful immobilization of AgNPs on the filter paper via direct immersion technique. The potential of the fabricated FP-AgNPs PAD as a colorimetric sensor for CN− was evaluated using water samples contaminated with various ions and CN− concentration. Here, a color change from yellow to colorless was instantly observed as the FP-AgNPs PAD was exposed to water samples containing CN−. Interestingly, no color change was observed for samples exposed to other analytes suggesting the good selectivity of the FP-AgNPs PAD. Ultraviolet–Visible spectroscopy results and digital image analysis revealed that the fabricated sensor can detect CN− with concentration down to 1.0 ppm. The colorimetric response was also obtained for real water samples spiked with CN−. The results stipulated in this work offer baseline information that can be used in developing highly selective and sensitive digital sensing devices for affordable, accessible, and fast water contaminant monitoring and other related applications.
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Ozer, T., McMahon, C., Henry, C.S.: Advances in paper-based analytical devices. Annu. Rev. Anal. Chem. 13, 85–109 (2020)
Fu, L.-M., Wang, Y.-N.: Detection methods and applications of microfluidic paper-based analytical devices. TrAC, Trends Anal. Chem. 107, 196–211 (2018)
Akyazi, T., Basabe-Desmonts, L., Benito-Lopez, F.: Review on microufluidic paper-based analytical devices towards commercialisation. Anal. Chim. Acta 1001, 1–17 (2018)
Sher, M., Zhuang, R., Demirci, U., Asghar, W.: based analytical devices for clinical diagnosis: recent advances in the fabrication techniques and sensing mechanisms. Expert Rev. Mol. Diagn. 17(4), 351–366 (2017)
Morbioli, G.G., Mazzu-Nascimento, T., Stockton, A.M., Carrilho, E.: Technical aspects and challenges of colorimetric detection with microufluidic paper-based analytical devices (µpads)-a review. Anal. Chim. Acta 970, 1–22 (2017)
Xia, Y., Si, J., Li, Z.: Fabrication techniques for microfluidic paper-based analytical devices and their applications for biological testing: a review. Biosens. Bioelectron. 77, 774–789 (2016)
Hendry-Hofer, T.B., Ng, P.C., Witeof, A.E., Mahon, S.B., Brenner, M., Boss, G.R., Bebarta, V.S.: A review on ingested cyanide: risks, clinical presentation, diagnostics, and treatment challenges. J. Med. Toxicol. 15(2), 128–133 (2019)
Jaszczak, E., Polkowska, Z., Narkowicz, S., Namiesnik, J.: Cyanides in the environment analysis problems and challenges. Environ. Sci. Pollut. Res. 24(19), 15929–15948 (2017)
Ma, J., Dasgupta, P.K.: Recent developments in cyanide detection: a review. Anal. Chim. Acta 673(2), 117–125 (2010)
Mohammadian, M., Sari, A.H.: Optical and morphological studies of gold nano thin fillms on glass substrates for surface plasmon resonance sensors. Int. J. Nano Res. 57, 51–57 (2019)
Shaikh, A.J., Batool, M., Yameen, M.A., Waseem, A.: Plasmonic e_ects, size and biological activity relationship of au-ag alloy nanoparticles. In: Journal of Nano Research 54, 98–111 (2018)
Sasikumar, T., Ilanchelian, M.: Colorimetric and visual detection of cyanide ions based on the morphological transformation of gold nanobipyramids into gold nanoparticles. New J. Chem. 44(12), 4713–4718 (2020)
Cheng, C., Chen, H.-Y., Wu, C.-S., Meena, J.S., Simon, T., Ko, F.-H.: A highly sensitive and selective cyanide detection using a gold nanoparticle-based dual fluorescence-colorimetric sensor with a wide concentration range. Sens. Actuators, B Chem. 227, 283–290 (2016)
Sadrolhosseini, A.R., Krishnan, G., Sa_e, S., Beygisangchin, M., Rashid, S.A., Harun, S.W.: Enhancement of the uorescence property of carbon quantum dots based on laser ablated gold nanoparticles to evaluate pyrene. Opt. Materials Express 10(9), 2227–2241 (2020)
Budlayan, M.L.M.B., Oracion, J.P.L., De La Rosa, L.B., Rodriguez, M.J., Patricio, J.N., Perez, S.J.L.N., Arco, S.D., Manigo, J.P., Austria, E.S., Alguno, A.A., Deocaris, C.C., Capangpangan, R.Y.: Preparation of spin-coated poly(vinyl alcohol)/chitosan/gold nanoparticles composites and its potential for colorimetric detection of cyanide in water. Pol. J. Environ. Stud. 31(2), 1–8 (2022)
Taheri, A., Noroozifar, M., Khorasani-Motlagh, M.: Investigation of a new electrochemical cyanide sensor based on ag nanoparticles embedded in a three-dimensional sol-gel. J. Electroanal. Chem. 628(1–2), 48–54 (2009)
Princy, K., Holaday, M.D., Gopinath, A.: Marine macroalgae bio-fabricated silver nanoparticles as naked-eye colorimetric and turn-on fluorescent sensor for cyanide ions in aqueous media. Environ. Nanotechnol. Monitoring Manage. 15, 100399 (2021)
Hassanvand, H., Hashemi, P.: Synthesis of silver nanoparticles-agarose composite and its application to the optical detection of cyanide ion. Anal. Sci. 34(5), 567–570 (2018)
Jiang, X., Fan, Z.H.: Fabrication and operation of paper-based analytical devices. Annu. Rev. Anal. Chem. 9, 203–222 (2016)
Budlayan, M.L.M., Patricio, J.N., Lagare, J.P.B., de la Rosa, L.B., Arco, S.D., Alguno, A.C., Austria, E.S., Manigo, J.P., Capangpangan, R.Y.: Functionalized silver nanoparticle-decorated paper sensor for rapid colorimetric detection of copper ions in water. Funct. Composites Struct. 3(3), 035007 (2021)
Budlayan, M.L., Lagare-Oracion, J.P., Rosa, L.D., Rodriguez, M.J., Manigo, J., Alguno, A., Austria, E., Arco, S., Patricio, J., Deocaris, C., Capangpangan, R.Y.: Gold nanoparticles-decorated paper-based sensor for rapid cyanide detection in water. Adv. Natl. Sci 12(2), 025007 (2021)
Elamawi, R.M., Al-Harbi, R.E., Hendi, A.A.: Biosynthesis and characterization of silver nanoparticles using trichoderma longibrachiatum and their effect on phytopathogenic fungi. Egypt. J. Biol. Pest Control 28(1), 1–11 (2018)
Anandalakshmi, K., Venugobal, J., Ramasamy, V.: Characterization of silver nanoparticles by green synthesis method using pedalium murex leaf extract and their antibacterial activity. Appl. Nanosci. 6(3), 399–408 (2016)
Hajizadeh, S., Farhadi, K., Forough, M., Sabzi, R.E.: Silver nanoparticles as a cyanide colorimetric sensor in aqueous media. Anal. Methods 3(11), 2599–2603 (2011)
Shengchun, Y., Shouan, D., Chun, T., Pinjiang, L.: Study on the reaction mechanism of silver nanoparticles with cyanide. Rare Metal Materials Eng. 34(8), 1243 (2005)
The authors would like to acknowledge the Commission on Higher Education (CHED)- DARE TO Program of the Philippines for funding this work.
This work was accomplished through the funding provided by Commission on Higher Education (CHED)-Philippines.
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Budlayan, M.L., Lagare-Oracion, J.P., Patricio, J. et al. Single-dip colorimetric detection of cyanide using paper-based analytic device based on immobilized silver nanoparticles. Int Nano Lett 12, 399–407 (2022). https://doi.org/10.1007/s40089-022-00373-1
- Silver nanoparticles
- Colorimetric sensing
- Paper-based analytical device