This study aimed to assess the effect of 4-nonylphenol (4-NP), a ubiquitous aquatic micro-chemical pollutant substance on some on some reproductive parameters and histology in koi carp. Fish were intraperitoneally subjected to ascending doses of 4-NP at 10, 50 and 100 μg/g body weight (BW) on days 7, 14 and 21 after the initiation of the experiment. Also, one group was treated with 17β-estradiol (E2) at 2 μg/g BW, positive control group (C2) received the vehicle (50 μL of coconut oil + 50 μL Ethanol) per fish and a negative control group (C1) was considered without injection. The results showed that 4-NP induced significantly an increase in E2, progesterone, and plasma vitellogenin at 50 μg/g in both male and female, while level of testosterone significantly decreased in the treated fish compared to control fish (P < 0.05). Conversely, the levels of these parameters at the higher concentration of 4-NP (100 μg/g) were significantly reduced compared to control one (P < 0.05). Also, some sever histopathological changes were observed in ovary, testicular and liver of treated fish, reflecting fish sensitivity to 4-NP at higher doses. This study showed that 4-NP has an estrogenic potency in the sexual development, hepatotoxicity and gonado-toxicity of koi carp.
Both natural and synthetic endocrine disrupting chemicals (EDCs) are able to cause many disorders and interferences with animal endogenous hormones systems including adverse effects on hormone receptors, normal hormone synthesis and secretion (Maradonna and Carnevali 2007; Zaccaroni et al. 2009). Huge volume of EDCs is released to the global environment influencing serious potential hazardous effects on animal health condition and reproductive functions via the food chain and contaminated water resources (Vos et al. 2000). Among classes of EDCs, 4-nonylphenol (4-NP) is considered as an estrogen-like and anti-androgenic chemical and captured the attention of many investigators to its effect on the animal reproductive system (Sayed et al. 2012). 4-NP is an ultimate degradation product of nonylphenol ethoxylates (NPEs) and both are the most widely used members of alkylphenol and alkylphenol ethoxylate family of non-ionic detergents used in a wide variety of industrial applications including pesticides, spermicides, paints, wetting agents, textiles, plastics and paper products (John et al. 2000; Uguz et al. 2003; Sone et al. 2004; Rivero et al. 2008).
Reproduction in vertebrates such as fish is mainly regulated by the endocrine system primarily affected along the hypothalamic–pituitary–gonadal (HPG) axis via neural and hormonal feedback (Yaron et al. 2003; Choi et al. 2010). The role of 17 β-estradiol (E2) as a neuroendocrine feedback control on the HPG axis has been shown in fish reproductive process (Yadetie et al. 1999). 4-NP has the ability to compete with E2 receptor binding and exerts an estrogen-like transcriptional activity on expression of estrogen receptor gene in liver, gill, pituitary and brain (Christensen et al. 1999; Yadetie et al. 1999). Several studies have been demonstrated an impairment of natural action of primary steroid hormones responsible for regulating gametogenesis including E2, androgens testosterone (T), and 11-ketotestosterone in fish collected from highly contaminated sites with alkylphenols or in fish after being exposed to nonylphenol ethoxylates (Sone et al. 2004; Rankouhi et al. 2004; Mitchelmore and Rice 2006). An increase in vitellogenin level in liver and blood of male common carp previously exposed to 4-NP has been also reported (Cardinali et al. 2004; Mitchelmore and Rice 2006). Moreover, histological alterations and lesions in reproductive organs as well as hepatic and kidney tissues have been reported in some fish species after being exposed to 4-NP (Weber et al. 2003; Zha et al. 2008; Yang et al. 2008). However, the harmful effects of 4-NP on fish reproductive system are still a controversial issue (Giesy et al. 2000; Matsumura et al. 2005; Yang et al. 2008).
This study aimed to investigate the effects of 4-NP as the biological breakdown product of widely used non-ionic surfactant on some reproductive parameters and histology of reproductive tissues in male and female of koi carp (Cyprinus carpio). As the HPG axis of this extensively farmed species is activated in small size (50–100 g), therefore, it is a good biological model for such physio-toxicological assays.
Materials and methods
One hundred and eighty apparently healthy immature koi carp including 90 females (55 ± 0.5 g) and 90 males (54 ± 0.7 g) were obtained from a local hatchery of an ornamental fish center (Tehran, Iran); and were transported to fisheries laboratory at Tehran Science and Research University. Fish were randomly divided into 18 glass aquaria (100 × 30 × 50 cm) with 10 fish per aquarium. Water quality condition (conductivity 400 L/min, pH 7.5, dissolved oxygen 5 ± 0.2 mg/L, temperature 23–24 °C and photoperiod of 12:12 h light:dark) was maintained constant during the trail with a whole water exchange per day and fish were fed with a commercial carp pellet at 3% body weight/day and waste products were siphoned daily. The experiment was performed according to the standard guidelines for the care and use of experimental animals by Science and Research Branch University Committee (Collegiate Committee on Ethics Matched Biomedical Researchers, Tehran).
Branched chain isomers of 4-NP (CAS No. 84852-15-3 with purity > 95%) and E2 (Sigma-Aldrich, Deisenhofen, Germany) were mixed with coconut oil (Sigma-Aldrich, Deisenhofen, Germany) and absolute ethanol (Merck, Darmstadt, Germany) as the chemical vehicle (Pait and Nelson 2003) to prepare treatment doses of 10, 50 and 100 μg/g body weight (BW) (Christensen et al. 1999; Yadetie et al. 1999; Casini et al. 2002; Carrera et al. 2007). E2 at 2 μg/g BW was also used as a separated treatment. Feeding was stopped 24 h prior to injection and ten fish per treatment were intraperitoneally injected with the mentioned dosages after being anesthetized with 2-phenoxyethanol at 0.1% (Merck, Darmstadt, Germany). The positive control group (C2) received the vehicle (50 μL of coconut oil + 50 μL Ethanol per fish) and a negative control group (C1) was considered without injection. Fish were injected on days 7, 14 and 21 after the initiation of the experiment.
On day 22, the blood samples were collected from the caudal vein after fish being anaesthetized with clove oil (150 mg/L), and sera samples were separated by centrifugation at 3000×g for 10 min (SIGMA 3–30 K, Osterode am Harz, Germany) before being frozen at − 80 °C until analysis. Fish were then dissected and separated by sex gonad characters.
Steroid hormones assay
Steroid hormone levels including E2, T, and P were measured using radio immune assay (RIA) kit (Immunotech, Marseille, France) and gamma counter (LKB-Wallac RackGamma, Turku, Finland). Samples and standards were incubated in monoclonal antibody-coated tubes with 125I-labeled tracer T, E2 and P (50 mL sample or standard and 500 mL of tracer). After one h incubation with horizontal shaking at 400 rpm, the liquid contents of the tubes were removed and the radioactivity bound to the antibody was measured by JNG 403 (Multichannel gamma counter for RIA). The samples were analyzed using two kits in two analyses’ forms of the calibration curve (r2 > 0.98).
The plasma vitellogenin (VTG) concentration was determined in the sera samples of five females and 5 males (totally 10 fish) in each treatment. VTG concentration was detected using carp vitellogenin ELISA kit (Biosense Laboratories AS, Bergen, Norway) following manufacturer’s instructions by an A3 plate reader (DAS, Rome, Italy) at 492 nm. VTG concentrations were detected based on a standard curve developed from the absorbance values for VTG standards (r2 > 0.99).
Five fish of each treatment were randomly removed and tissue samples of ovary, testis and liver were taken prior to be fixed in 10% neutral buffered formalin. The fixed tissues were processed for routine histo-technique, embedded in paraffin blocks before obtaining 5 μm thickness sections by a Leica RM2255 microtome (Leica Microsystems, Germany). The obtained sections were stained with Harris’s hematoxylin and eosin stain (H&E), dehydrated through a graded alcohol series and mounted onto the glass slides (Bancroft and Stevens 1982) before being examined under light microscope (Olympus DP71, Tokyo, Japan).
Transmission electron microscope (TEM)
To evaluate TEM, the ovaries were cut into approximately 1 × 1 mm piece, fixed in 3% glutaraldehyde and washed in cacodylate buffer prior to being fixed in 1% osmium tetroxide prepared in the same buffer for one h at 4 °C. Specimens were then dehydrated in ascending grades of acetone with two changes of 15 min each after being cleared by propylene oxide for 30 min. Infiltration was performed gradually in different proportion of propylene oxide with liquid resin. Tissue samples were embedded in the araldite embedding medium using BEEM® capsules (BEEM Inc., Bronx, USA) before being incubated at 50 and 60 °C in an embedding oven for 24 and 48 h, respectively, to complete polymerization. The obtained thin sections (1 μm) were stained with toluidine blue before being used for examination under a light microscope. The ultrathin sections collected on copper grids were also stained with uranyl acetate followed by lead nitrate prior to examination under scanning electron microscopy (Oxford INCA 200, Oxford Instruments Group, High Wycombe, UK) at an accelerating voltage of 80 kV.
Data were subjected to SPSS Version 16.0 for windows (SPSS Inc., USA), and checked for normal distribution (Shapiro–Wilk’s test) and homogeneity of variances (Levene’s test) using P–P plot analysis. One-way analysis of variance (ANOVA) followed by the NP exposure for each endpoint relative Duncan’s test was used to examine the effect of the control group and significance was set at P < 0.05.
The value of E2 in female fish received 10 and 50 µg 4-NP were significantly (P < 0.05) higher than control groups (Fig. 1), while fish exposed to 100 µg 4-NP showed significantly (P < 0.05) a lower value of E2 than both 10 and 50 µg 4-NP treatments (Fig. 1). Also, the value of T was decreased with an increase in 4-NP dose (P < 0.05) with the lowest value obtained in fish treated with 100 µg 4-NP (P < 0.05) (Fig. 1). Also, E2 value in E2-treated fish group was significantly lower than fish treated with 4-NP at 50 µg/g BW, while it increased to higher levels than 10 and 50 µg 4-NP groups. Also, no significant difference was seen in the level of P between 10 and 50 µg 4-NP groups, while this was significantly lower in the 100 µg 4-NP group. No significant difference was seen in level of T between E2 treated fish and 4-NP treated with 100 µg/g BW (P < 0.05), while T level in E2-treated fish was lower than 10 and 50 µg/g BW groups. Also, E2 value in fish treated with 2 μg E2 was significantly (P < 0.05) higher than control groups.
The highest value of E2 was obtained in fish treated with 4-NP at 50 µg compared to other groups and control ones (P < 0.05). Also, this value in fish treated with 2 µg E2 was significantly higher than fish treated with 10 and 100 µg4-NP (P < 0.05) (Fig. 1). The concentration of E2 in fish treated with 10 and 50 µg 4-NP and 2 µg E2 showed significantly a higher value than both control groups. In addition, the value of P increased significantly (P < 0.05) with an increase in 4-NP concentration up to 50 µg, but its concentration at 100 µg 4-NP gave a significant decrease compared to both 10 and 50 µg treatments (Fig. 1). No significant difference was seen in level of P among fish treated with 100 µg 4-NP, 2 µg E2 and control groups (P > 0.05). Value of T in fish treated with 10 and 50 µg 4-NP was significantly higher than fish treated with 100 µg 4-NP and 2 µg E2. Also, this value in both fish treated with 100 µg 4-NP and 2 µg E2 was significantly lower than control groups (P < 0.05).
The plasma VTG concentration was significantly higher in fish treated with 10 and 50 µg 4-NP/g BW compared to controls groups (P < 0.05). Also, fish received 100 µg 4-NP significantly showed a decrease in VTG value compared to both 10 and 50 µg 4-NP trails (P < 0.05). Further, fish treated with 2 μg E2 significantly gave an increase in VTG level compared to other groups (Fig. 2).
A similar trend in plasma VTG concentration was observed in male fish (Fig. 2). VTG concentration significantly (P < 0.05) increased in fish treated with 10 µg 4-NP compared to control groups. Considerable elevations were also observed in plasma concentration of male fish exposed to 50 µg 4-NP/g BW compared to other treatments (P < 0.05), but a significant decrease was observed in 100 μg 4-NP trail compared to both 10 and 50 µg 4-NP trails (Fig. 2).
Female histological findings
No histological changes were seen in the ovaries of control fish (Fig. 3a, b). The ovary of female fish exposed to 10 and 50 µg 4-NP was loaded with oocytes in different vitelogenic stages and late vitellogenesis (Fig. 3c, d). The vitellogenic oocytes contained normal yolk materials (fatty yolk globules and protein granules) and surrounded by zona radiate and follicular epithelium that consists of granulose cell layer and thecal layer. Fish exposed to 100 µg 4-NP showed a reduction in size of the growing vitellogenic oocytes and ova damage including vacuoles (v), irregular shape of ovum with rupture of follicular layer and atretic (Fig. 3e, f). The number of follicular atretic after exposure to 100 µg 4-NP was significantly increased compared to control fish (arrows). No histological change was seen in ovaries of control fish (Fig. 3a, b).
Necrosis, degeneration, psychosis, and cytoplasmic vacuolization of hepatic parenchyma were seen in the liver samples all treated fish (Fig. 4b, c) but with more severe histological changes observed in fish treated with higher dosage of 4-NP, i.e., 100 µg/g BW (Fig. 4d). Also, liver samples of fish treated with 100 µg 4-NP showed a sedimentation of cytoplasmic and an increase in cytoplasmic vacuolization (Fig. 4e, f).
No abnormality was seen in sections obtained from the control fish (Fig. 5a). Ovaries of female fish exposed to 10 µg 4-NP showed an increase in size and number of previtellogenic oocytes and most of ova demonstrated a vitellogenesis stage containing droplets of fat and cortical alveoli. The external membrane presented a normal in shape having a higher amount of yolk particles. Yolk particles with a relatively high volume of pores and channels through the porous passing to the inner membrane and density of porous channels were also seen (Fig. 5b). In fish exposed to 50 µg 4-NP, an increase in vitellogenesis with a remarkable increase in vitellogenin particles was observable. Outer membrane of the yolk particles with a relatively high volume of pore channels in passing to the inner membrane and making micro-pinocytosis cascade was seen (Fig. 5c, d). An abnormal external membrane with a few channels containing a little penetration of vitellogenin inside the internal membrane was also seen in fish exposed to 100 µg 4-NP. Further, a decrease in number of channels with no transport of materials in this layer was observable (Fig. 5e, f).
Male histological findings
The testis of the control fish contained spermatids, spermatozoa, lumen space, seminiferous lobule, and interlobular connective tissue histological structure (Fig. 6a). In testis of fish exposed to 10 µg 4-NP, a large extended sperm duct loaded with spermatozoa and seminiferous lobules with different amounts of spermatozoa, together with wide lumen free of spermatozoa, were detected (Fig. 6b). In fish exposed to 50 µg 4-NP, a discontinuous interlobular connective tissue with a reduction in testis mass was seen. Also, the seminiferous lobules were filled with spermatozoa or late spermatids (Fig. 6c). A decrease in spermatids and spermatozoa, degeneration of Leydig cells, atrophy of testis cells and cellular vacuolation were also observable in sections obtained from fish treated with 100 µg 4-NP (arrows) (Fig. 6d).
The results of the present work showed that 4-NP at 10 and 50 µg/g BW was able to increase the concentrations of steroid hormones of E2 and P in both male and female koi carp, while the value of testosterone was reduced. However, these hormones’ concentrations were reduced at the higher dosage, i.e., 100 μg/g BW. Hasselberg et al. (2004) showed that there is a reverse relationship between CYP1A and amount of estradiol in fish plasma in a way that CYP1A is effective in metabolizing steroids. In our study, a gradual increase in E2 of fish treated with 10 and 50 µg/g BW could be a reason for a decrease in the activity of metabolizing enzymes. Yang et al. (2008) reported similar results when goldfish (Carassius auratus) was exposed to 4-NP at 100 µg 4-NP for 20 days. Also, a significant increase of E2 was seen in female fathead minnows (Pimephales promelas) after fish being exposed to 4-NP at 0.09 µg/L (Giesy et al. 2000). Lavado et al. (2004) demonstrated an inhibition in glucuronidation and sulfidation of sexual steroids in common carp exposed to NP. Thus, such inhibition of glucuronidation and sulfidation in liver could be the cause of an increase in E2 seen in our work. However, when turbot (Scophthalmus maximus) was exposed to nonylphenol at 30 µg/g significantly, a decrease was occurred in the estradiol and testosterone levels as well as a reduction in the ovary aromatase enzyme and glucuronidation of sex steroids (Martin-Skilton et al. 2006).
Zenoestrogens like 4-NP can be accomplished by inhibitory effects in different sections of hypothalamus–hypophysis–gonads axis, and an effluence of the hormone receivers can cause a change in the secretion of hormones (Folmar et al. 2002; Lavado et al. 2004). Also, 4-NP could inhibit the activity of 17 alpha-hydroxylase enzymes which are involved in testosterone synthesis (Laurenzana et al. 2002). However, Villeneuve et al. (2002) have demonstrated no effect on E2, T and VTG in matured common carp male exposed to 4-NP at 0.05–5.4 µg/L for 28–31 days.
The plasma VTG in both male and female fish treated with 50 µg/g BW was significantly higher than control fish, whereas it significantly decreased at higher concentration, i.e.100 µg/g BW that may be in part due to a higher level of estrogen receptors on hepatocytes. Flouriot et al. (1995) demonstrated an increase in level of estrogen receptors and mRNA vitellogenin in hepatocyte cells of rainbow trout after fish being exposed to 4-NP at 10 µM. Therefore, 4-NP may play a role via estrogen receptors in a competition pathway with a much lower potent than E2 providing an estrogenic effect. Such similar function of 4-NP with E2 mimics to enhance the vitellogenin synthesize resulting in both hyperplasia and hypertrophy of hepatocytes in liver tissue (Arukwe et al. 1997).
Remarkable histological malformations were observed in ovaries of koi carp exposed to 4-NP, especially at the higher dosage. This could be in part due to 4-NP effect on oocytes and intrigue of gene expression of VTG as well as an increase in yolk formation and growth of ovary follicles. An increase in yolk production can cause a gathering vitellogenesis in oocytes with a consequence increasing in GSI index in female fish. In contrast, number of degenerated eggs and asteriated follicles in the ovary fish subjected to 4-NP at 100 µg/g BW showed a significant increase compared to other treatments. Degeneration of the cells of the follicular layer may cause a decrease in the level of synthesis of sexual steroids delaying the maturity of sexual cells and egg expansion. Holdway et al. (2008) reported that exposing fish to EDCs before the spawning can result in a change in reproduction behavior, decrease fecundity and sexual hormone excretion. Also, Sayed et al. (2014) demonstrated a structural pathology and function in Nile tilapia (Oreochromis niloticus) with a high decrease in gonadosomatic indexes, fecundity and oocyte diameter after fish being exposed to 100 µg 4-NP. In addition, African catfish (Clarias gariepinus) subjected to 4-NP at 0.1 mg/L showed a decrease in fertilization, egg hatching and fecundity as well as a remarkable reduction in incubation period, mortality and abnormality of embryos (Sayed et al. 2012). In the present study, liver histology of female koi carp exposed to 4-NP demonstrated several cellular disorders including degeneration, necrosis and vacuolation. It has been shown by Lee et al. (2003) that 4-NP is able to create such disorders in testicules structures. Also, similar histopathological signs have been also reported in different fish spices subjected to NP at different concentrations (Lee et al. 2004; Bhattacharya et al. 2008; Kaptaner and Ünal 2011). However, lower concentration of 4-NP can promote sexual glands preparatory phase in female catfishe (Heteropneustes fossilis) by ovulation and maturation of oocytes (Gautam et al. 2011). Therefore, NP at lower concentration can cause ovulation and maturation of oocytes, but at the higher concentrations a delay in maturation and growth of sexual glands together with a significant increase in atresia oocytes of stages 1 and 2 can be occurred.
In conclusion, the results of this study demonstrated that 4-NP can mimic to play a role as an estrogenic hormone in the sexual development, hepatotoxicity and gonado-toxicity in koi carp. Also, 4-NP demonstrated to induce vitellogenin production and interfere with fish gonadal maturation. Therefore, as a large volume of detergents containing 4-NP are currently discharged into aquatic ecosystems, a particular attention is required to avoid use of contaminated waters with 4-NP for aquaculture activities that can lead to failure in the propagation and rearing of both wild and commercial fish species.
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Authors would like to thank Mr. Hamid Fathalian and Mr. Saeid Totoonchi Mashhor for their valuable assistance.
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Amaninejad, P., Hosseinzadeh Sahafi, H., Soltani, M. et al. Endocrine disrupting effects of 4-nonylphenol on plasma vitellogenin, reproductive system and histology in koi carp (Cyprinus carpio). Int Aquat Res 10, 263–274 (2018). https://doi.org/10.1007/s40071-018-0203-8
- Reproductive system
- Koi carp