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A novel high-throughput single-molecule technique: DNA curtain

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Abstract

Single-molecule (SM) techniques have been contributing to unraveling the detailed mechanisms underlying DNA metabolic processes with superior spatiotemporal resolution beyond the limit of traditional biochemical assays. However, SM techniques need to gather a great deal of data to guarantee statistical reliability. The ‘DNA curtain’ technique is a high-throughput system integrating fluorescence imaging, microfluidics, and nano-engineering to enable the visualization of protein-DNA interactions at the single-molecule level. Since the DNA curtain technique was presented, it has evolved for probing diverse DNA transactions including replication, repair, recombination, and chromatin dynamics. Here, we introduce several types of DNA curtain assays and review their principles, technical details, and applications.

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  • 21 January 2021

    The article was revised to change copy right year.

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Acknowledgements

The authors would like to thank Na Young Cheon and Yujin Kang for thoughtful discussion and DNA curtain images. Ja Yil Lee is financially supported from a National Research Foundation (grant number: NRF-2020R1A2B5B01001792) and the Institute for Basic Science (IBS-R022-D1).

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Authors and Affiliations

  1. Department of Physics and Astronomy, Seoul National University, Seoul, 08826, South Korea

    Jongjin Cha

  2. Department of Biological Sciences, Ulsan National Institute of Science and Technology, Ulsan, 44919, South Korea

    Ja Yil Lee

  3. Center for Genomic Integrity, Institute for Basic Science, Ulsan, 44919, South Korea

    Ja Yil Lee

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  1. Jongjin Cha
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Correspondence to Ja Yil Lee.

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Cha, J., Lee, J.Y. A novel high-throughput single-molecule technique: DNA curtain. J. Korean Phys. Soc. 78, 442–448 (2021). https://doi.org/10.1007/s40042-020-00031-9

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