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Development of an Experimental Model of Vasculitis Using Ovalbumin Lipopolysaccharide in Rats

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Vasculitis is considered a veiled threat for several pathologic conditions, and consequently, there is a need to develop a distinct animal model for drug testing. An adjunct use of antigens, ovalbumin (OVA), and lipopolysaccharide (LPS) has been reported in exaggerating inflammation. However, the effects of OVA and LPS individually or in combination have not yet been established in inducing vasculitis. The present study examined effects of OVA and LPS on vasculitis induction in rats. Rats treated with OVA (5 mg/kg, i.p.) and LPS (1 mg/kg, i.p.) showed a significant increase in circulating inflammatory cells, erythrocyte sedimentation rate, inflammatory cytokines (IL-1β, IL-6, and TNF-α), c-reactive protein, anti-neutrophil cytoplasmic antibodies (anti-myeloperoxidase (MPO), anti-proteinase-3 (PR3)), liver function enzymes (AST, ALT), and kidney damage markers (BUN, Creatinine) in the serum. Matrix metalloproteinase (MMP)-9 levels were significantly increased in the temporal, carotid, aortic, iliac, mesentery, and coronary arteries. In addition, the diseased group developed hematuria and proteinuria, which was incomparable to the normal group. Histopathology further revealed significant neutrophil infiltration accompanied by fibrinolytic necrosis, indicating vascular injury and hyperplasia, leading to extracellular matrix degradation. In conclusion, the combination of ovalbumin and lipopolysaccharide has developed vasculitis-like conditions, which suggest OVA-LPS can be a new experimental model for vasculitis and be considered in future therapeutic studies.

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Acknowledgements

The present study was supported by grant from Student Startup Innovation Policy (SSIP) scheme (Grant Number 215/2019), Government of Gujarat, India.

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  1. Department of Pharmacology, L. M. College of Pharmacy, Gujarat Technological University, Navrangpura, Ahmedabad, Gujarat, 380009, India

    Vandana R. Thakur & Anita A. Mehta

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  1. Vandana R. Thakur
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  2. Anita A. Mehta
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Correspondence to Anita A. Mehta.

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A new animal model for Vasculitis has been established using OVA and LPS, which were previously used only as adjuncts to exaggerate the inflammatory process in Vasculitis. OVA and LPS have suggested a possible simplified animal model for vasculitis.

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Thakur, V.R., Mehta, A.A. Development of an Experimental Model of Vasculitis Using Ovalbumin Lipopolysaccharide in Rats. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. (2024). https://doi.org/10.1007/s40011-024-01548-w

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  • DOI: https://doi.org/10.1007/s40011-024-01548-w

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