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Neuroprotective Effect of Cyclosporine Against Oxidative Stress-Induced Neurotoxicity in PC12 Cells

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Oxidative stress has crucial role in neuronal cell death associated with neurodegenerative diseases including Alzheimer's disease. Excessive levels of reactive oxygen species (ROS) and induction of apoptosis are thought to lead to neuronal cell death. The aim of this study was to evaluate the effect of cyclosporine A (CsA) on hydrogen peroxide (H2O2)-elicited oxidative damage in PC12 cells. The cells were incubated with CsA and then treated with H2O2 for 24 h. The viability of cells was measured by MTT assay. Moreover, lipid peroxidation (LPO), total thiol molecules (TTM), and total antioxidant capacity (TAC) were evaluated. Our results demonstrated that CsA notably improved the reduced viability of PC12 cells induced by H2O2. Treating PC12 cells with H2O2 increased LPO level, whereas CsA significantly reduced LPO. CsA effectively increased the TTM and TAC level, as compared with H2O2 group. These findings suggested that CsA has beneficial effects against H2O2-mediated damage in PC12 cells.

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Acknowledgements

This study was supported by Deputy of Research of Kerman University of Medical Sciences, Kerman, Iran (Grant number: 96001031).

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Authors and Affiliations

  1. Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran

    Azadeh Liravi

  2. Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

    Mehrnaz Mehrabani

  3. Department of Pharmacology and Toxicology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran

    Somayyeh Karami-Mohajeri & Azadeh Aminzadeh

  4. Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

    Somayyeh Karami-Mohajeri & Azadeh Aminzadeh

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  1. Azadeh Liravi
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  2. Mehrnaz Mehrabani
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  4. Azadeh Aminzadeh
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Correspondence to Azadeh Aminzadeh.

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Significance Statement: Oxidative stress usually implicated in neurodegenerative diseases, including Alzheimer's disease. Our study showed the beneficial effects of cyclosporine on hydrogen peroxide-mediated damage in PC12 cells.

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Liravi, A., Mehrabani, M., Karami-Mohajeri, S. et al. Neuroprotective Effect of Cyclosporine Against Oxidative Stress-Induced Neurotoxicity in PC12 Cells. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 94, 41–45 (2024). https://doi.org/10.1007/s40011-023-01505-z

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  • DOI: https://doi.org/10.1007/s40011-023-01505-z

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