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Evaluation of Thermostable Biomolecule Cocktail from Algal-associated Hot Water Spring Bacteria for Antibiofilm Activities

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Controlling bacterial biofilms is a major target for industrial processes and thus is a tedious task under study in many research proceedings. The algal associated bacterial isolates from Unai Mata hot water spring, Gujarat, India, were screened for production of potent amylases, proteases and biosurfactants. The partially purified biomolecules were checked for effects of substrate concentration, cations and stability at extreme physical conditions like temperature (50 ℃) and acidic pH (5 and 6). Metal ions namely—Cu and Zn for amylase, while Zn and Na for protease, were found to be yielding least and highest activities, respectively. Best three isolates were selected for potential biomolecule production and sequenced for 16 s rDNA gene. The three isolates were found to be Stenotrophomonas sp. strain T1UM1, Pantoea sp. strain T1UM4 and Bacillus sp. strain T1UM8 with GenBank accession number MH764436, MH764437 and MH764438, respectively. The partially purified biomolecules cocktail showed effective antibiofilm activities at concentration of 1, 2, 5 and 10 U/ml or mg/ml against various bacteria tested at 1, 2 and 5 h treatments. The effects were different on the type of bacterium containing a combination of specific biomolecules. Thus, biomolecule cocktail reported here from hot water spring isolates has antibiofilm application potential in industries.

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Acknowledgements

The authors would like to thank the Provost and administrative staff from Uka Tarsadia University for their financial support (Research Promotion Scheme) during the work and for their constant support.

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  1. Anoop R. Markande

    Present address: Department of Biological Sciences, P.D. Patel Institute of Applied Sciences, Charotar University of Science and Technology, Changa, 388 421, Anand, Gujarat, India

Authors and Affiliations

  1. C.G. Bhakta Institute of Biotechnology, Uka Tarsadia University, Maliba Campus, Bardoli Mahuva RoadSurat district, Tarsadi, 394 350, Gujarat, India

    Mubassira K. Shaikh, Divya D. Patel, Shraddha C. Dobariya & Anoop R. Markande

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  1. Mubassira K. Shaikh
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  2. Divya D. Patel
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  3. Shraddha C. Dobariya
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Contributions

MS, DP and SD worked on Amylase, Biosurfactants and Protease production, respectively, under the guidance of AM who also prepared the basic manuscript.

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Correspondence to Anoop R. Markande.

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Significance statement Use of biomolecules from extreme condition isolates yields immense industrial potential as they are expected to be viable for a wide range of temperatures and pH. The present report of enzymatic and biosurfactant cocktails for the removal of biofilms has potent industrial and environmental applications.

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Shaikh, M.K., Patel, D.D., Dobariya, S.C. et al. Evaluation of Thermostable Biomolecule Cocktail from Algal-associated Hot Water Spring Bacteria for Antibiofilm Activities. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 93, 409–418 (2023). https://doi.org/10.1007/s40011-022-01432-5

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  • DOI: https://doi.org/10.1007/s40011-022-01432-5

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