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Morpho-physiological Responses of Finger Millet Genotypes to PEG-Induced Osmotic Stress at an Early Seedling Stage

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Abstract

The present study reports the effect of polyethylene glycol (PEG) (0–25%) induced osmotic stress on morpho-physiological responses of finger millet Eleusine coracana (L.) Gaertn] genotypes at an early seedling stage. The significant differences in morpho-physiological responses were observed at 25% PEG over control, 15–20% PEG concentration. At 25% concentration, finger millet genotypes were classified into three groups according to their F values. The first group contains seven sensitive genotypes GE 811, GPU 28, MS 9272, AMM 679, GE 4962, BM and PR 4617, with contribution from germination percentage, germination rate, root length, seed vigor index and tissue water content. The second group contains nine tolerant genotypes AMM 197, GE 4434, GE 496, GE 1013, GE 1332, GE 4764, GPU 67, PR 202, RAU 8 with contribution from fresh weight, dry weight, root: shoot ratio, chl a, chl b, total chlorophyll, and CSI. The third group consists of two semi-sensitive genotypes, PR 6038 and KEP 534 contributed by root length and seedling length. This study provides a rapid and reliable method for screening finger millet genotypes using germination, morpho-physiological parameters, and multivariate analysis to support future breeding programs.

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References

  1. Vetriventhan M, Upadhaya HD, Dwivedi SL, Pattanashetti SK, Singh SK (2016) Finger and foxtail millets. In: Singh M, Upadhyaya HD (eds) Genetic and genomic resources for grain cereals improvement. Academic Press, Cambridge, pp 291–319

    Chapter  Google Scholar 

  2. Mukami A, Ngetich A, Mweu C, Oduor RO, Muthangya M, Binda WM (2019) Differential characterization of physiological responses during drought stress in finger millet varieties. Physiol Mol Bio Plant 25:837–846. https://doi.org/10.1101/603944

    Article  CAS  Google Scholar 

  3. Mundada PS, Nikam TD, Anil Kumar S, Umdale SD, Ahire ML (2020) Morphophysiological and biochemical responses of finger millet (Eleusine coracana (L.) Gaertn.) genotypes to PEG-induced osmotic stress. Bio Agr Bio 23:101488. https://doi.org/10.1016/j.bcab.2019.101488

    Article  Google Scholar 

  4. Khan MN, Zhang J, Luo T, Liu J, Ni F, Rizwan M, Fahad S, Hu L (2019) Morpho-physiological and biochemical responses of tolerant and sensitive rapeseed cultivars to drought stress during early seedling growth stage. Acta Physiol Plant 41:25. https://doi.org/10.1007/s11738-019-2812-2

    Article  CAS  Google Scholar 

  5. Bhatt D, Negi M, Sharma P, Saxena SC, Dobriyal AK, Arora S (2011) Responses to drought induced oxidative stress in five finger millet varieties differing in their geographical distribution. Physiol Mol Bio Plant 17:347–353. https://doi.org/10.1007/s12298-011-0084-4

    Article  CAS  Google Scholar 

  6. Munns R, Guo J, Passioura JB, Cramer GR (2000) Leaf water status controls day-time but not daily rates of leaf expansion in salt-treated barley. Aus J Plant Phy 27:949–957. https://doi.org/10.1071/PP99193

    Article  Google Scholar 

  7. Parvathi MS, Nataraja K (2017) Discovery of stress responsive TATA box binding protein associated factor6 (TAF6) from finger millet (Eleusine coracana (L.) Gaertn.). J Plant Bio 60:335–342. https://doi.org/10.1007/s12374-016-0574-6

    Article  CAS  Google Scholar 

  8. Zhu JK, Hasegawa PM, Bressan RA (1997) Molecular aspects of osmotic stress in plants. Cri Rev Plant Sci 16:253–277. https://doi.org/10.1080/07352689709701950

    Article  CAS  Google Scholar 

  9. Huang B, Su J, Zhang G, Luo X, Wang H, Gao Y, Ma G, Wang J, Cai D, Zhang X, Huang B (2015) Screening for Eruca genotypes tolerant to polyethylene glycol-simulated drought stress based on the principal component and cluster analyses of seed germination and early seedling growth. Plant Gene Res 15:87–193. https://doi.org/10.1017/S1479262115000519

    Article  CAS  Google Scholar 

  10. Razzaq H, Tahir N, Hammad M, Sadaqat HA, Sadia B (2017) Screening of sunflower (Helianthus annuus L.) accessions under drought stress conditions, an experimental assay. J Soil Sci Plant Nut 17:662–671. https://doi.org/10.4067/S0718-95162017000300009

    Article  CAS  Google Scholar 

  11. Michel BE, Kaufman MR (1973) The osmotic pressure of polyethylene glycol 6000. Plant Physiol 51:914–916. https://doi.org/10.1104/pp.51.5.914

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Carleton AE, Cooper CS, Wiesner, (1968) LE Effect of seed pod and temperature on speed of germination and seedling Elongation of Sainfoin (Onobrychis viciaefolia Scop.). Agro J 60:81–84. https://doi.org/10.2134/agronj1968.00021962006000010026x

    Article  Google Scholar 

  13. Abdul-Baki AA, Anderson JD (1973) Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci 13:227–232. https://doi.org/10.2135/cropsci1973.0011183X001300020023x

    Article  CAS  Google Scholar 

  14. Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in beta vulgaris. Plant physiol 24:1–15. https://doi.org/10.1104/pp.24.1.1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Murthy KS, Mujumdhar SK (1962) Modification of the technique for determination of chlorophyll stability index in relation to studies of drought resistance in rice. Cur Sci 31:470–471. https://doi.org/10.18805/LR-4314

    Article  Google Scholar 

  16. Allel D, Ben-Amar A, Badri M, Abdelly C (2016) Salt tolerance in barley originating from harsh environment of North Africa. Aus J Crop Sci 10:438–451

    Article  Google Scholar 

  17. Hegarty TW (1977) Seed activation and seed germination under moisture stress. New Physiol 78:349–359. https://doi.org/10.1111/j.1469-8137.1977.tb04838.x

    Article  Google Scholar 

  18. Hunter JR, Erickson AE (1952) Relation of seed germination to soil moisture tension. Agro J 44:107109. https://doi.org/10.2134/agronj1952.00021962004400030001x

    Article  Google Scholar 

  19. Singh D, Dobey A, Verma AK (2018) Evolution of morphological and physiological parameters during drought stress in Barnyard millet growing in Kumaon region of Himalaya. Res J Chem Env Sci 6:64–85

    CAS  Google Scholar 

  20. Kumari A, Sairam RK, Singh SK, Krishna HK (2014) Early growth response: an indicator of subsequent growth and yield of wheat genotypes grown under simulated water stress condition. Ind J Plant Physiol 19:94–100. https://doi.org/10.1007/s40502-014-0077-8

    Article  Google Scholar 

  21. Hellal FA, El-Shabrawi HM, El-Hady MA, Khatab IA, El-Sayed SAA, Abdelly C (2018) Influence of PEG induced drought stress on molecular and biochemical constituents and seedling growth of Egyptian barley cultivars. J Gen Eng Biotech 16:203–212. https://doi.org/10.1016/j.jgeb.2017.10.009

    Article  CAS  Google Scholar 

  22. Saha RR, Hannan A, Nessa A, Malek MA, Islam MR (2017) Selection of drought tolerant wheat genotypes by osmotic stress imposed at germination and early seedling stage. SAARC J Agr 15:177–192. https://doi.org/10.3329/sja.v15i2.35147

    Article  Google Scholar 

  23. Kholova J, Hash CT, Kocova M, Vadez V (2011) Does a terminal drought tolerance QTL contribute to differences in ROS scavenging enzymes and photosynthetic pigments in pearl millet exposed to drought? Env Exper Bot 71:99–106. https://doi.org/10.1016/j.envexpbot.2010.11.001

    Article  CAS  Google Scholar 

  24. Zhu XH, Song YC, Zhao ZH, Shi YS, Liu YH, Li Y, Wang TY (2008) Methods for identification of drought tolerance at germination period of foxtail millet by osmotic stress. J Plant Gen Res 9:62–67

    CAS  Google Scholar 

  25. Sareeta N (2017) Chlorophyll stability: a better trait for grain yield in rice under drought. Ind J Ecol 44:77–82

    Google Scholar 

  26. Ahmad NS, Kareem SH, Mustafa KM, Ahmad DA (2017) Early screening of some Kurdistan wheat (Triticum aestivum L.) cultivars under drought stress. J Agric Sci 9:88–103. https://doi.org/10.5539/jas.v9n2p88

    Article  Google Scholar 

  27. Sadeghian SY, Yavari N (2004) Effect of water-deficit stress on germination and early seedling growth in sugarbeet. J Agro Crop Sci 190:138–144. https://doi.org/10.1111/j.1439-037X.2004.00087.x

    Article  Google Scholar 

  28. Arunyanark A, Jogloy S, Akkasaeng C, Vorasoot N, Kesmala T, Nageswara Rao RC, Wright GC, Patanothai A (2008) Chlorophyll stability is an indicator of drought tolerance in peanut. J Agro Crop Sci 194:113–125. https://doi.org/10.1111/j.1439-037X.2008.00299.x

    Article  CAS  Google Scholar 

  29. Chikha MB, Hessini K, Ourteni RN, Ghorbel A, Zoghlami N (2016) Identification of barley landrace genotypes with contrasting salinity tolerance at vegetative growth stage. Plant Biotech 33:287–295. https://doi.org/10.5511/plantbiotechnology.16.0515b

    Article  Google Scholar 

  30. Kadir K, Talebi R, Hamidi H (2017) Multivariate analysis and drought stress tolerance indices in chickpea (Cicer arietinum L.) under different irrigation regimes. J Exp Bio Agr Sci 5:54–60. https://doi.org/10.18006/2017.5(1).054.060

    Article  Google Scholar 

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Acknowledgements

The financial support for this research was provided by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), Government of India, New Delhi, India, in the framework of the Extramural Research Scheme [EMR/2015/000145]. In addition, MLN expresses thanks to the National Fellowship for Scheduled Tribes (NFST) for awarding the fellowship. The authors also thank Dr. H.D. Upadhyaya, ICRISAT, Patancheruvu, Hyderabad, India and Dr. Prabhakar, Former Project Coordinator, AICRP on Small Millets, University of Agricultural Sciences, GKVK, Bangalore, India, for providing seeds of different genotypes of finger millet.

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Authors and Affiliations

  1. Department of Botany, Yogi Vemana University, Kadapa, 516005, Andhra Pradesh, India

    Lakshmipathi Naik Mude, Muniraja Mondam, Vijayalakshmi Gujjula & Shaik Sha Valli Khan Patan

  2. Department of Genetics and Genomics, Yogi Vemana University, Kadapa, 516005, Andhra Pradesh, India

    Sivakumar Jinka & Osman Basha Pinjari

  3. Crop Improvement, International Center for Genetic Engineering and Biotechnology,, Jawaharlal Nehru University, Aruna Asif Ali Marg, Delhi , 110067, New Delhi, India

    Varakumar Panditi

  4. Department of Crop physiology, University of Agricultural Sciences, GKVK, Bangalore, 560065, Karnataka, India

    YA Nanja Reddy

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  1. Lakshmipathi Naik Mude
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Significance statement: This study provides a rapid and reliable PEG-induced osmotic stress method for screening finger millet genotypes in future breeding programs.

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Mude, L.N., Mondam, M., Gujjula, V. et al. Morpho-physiological Responses of Finger Millet Genotypes to PEG-Induced Osmotic Stress at an Early Seedling Stage. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 93, 337–350 (2023). https://doi.org/10.1007/s40011-022-01421-8

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