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Green synthesis of silver nanoparticles using Momordica cymbalaria aqueous leaf extracts and screening of their antimicrobial activity

AgNPs studies in Momordica cymbalaria

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Biosynthesis of silver nanoparticles (AgNPs) is an easy, environmentally benign or eco-friendly process with very less or no cost and is having immense applications in biotechnological, biomedical, agricultural and environmental fields. In the present investigation, AgNPs were successfully synthesized for the first time using an aqueous leaf extract of Momordica cymbalaria from 6 mM silver nitrate solution. The synthesis of AgNPs was preliminarily confirmed by the color change of the reaction mixture and further confirmed and characterized by UV–Vis spectroscopy, FTIR, SEM and XRD. The UV–Vis spectrum confirmed the presence of AgNPs between the wavelength range of 300–600 nm, with the highest peak area of 422 nm. XRD and SEM analyzes showed an average particle size of 16.58 nm with smooth-surface spherical-shaped silver nanoparticles. The FTIR spectrum analysis of silver nanoparticles reveals different functional groups such as primary amines (–NH2), secondary amines (–NH–), aldehydes (–CHO), ketone (C=O), carboxylic acid (–COOH), alkynes and SP3  carbons respectively. Furthermore, these biosynthesized nanoparticles showed antibacterial activity against multidrug-resistant human pathogens.

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Acknowledgements

The authors duly acknowledge the Department of Biotechnology, Kakatiya University, Warangal, for providing all the facilities for carrying out the research. The authors are thankful to UGC, SAP-DRS-II, NEW DELHI, India, for financial support.

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Authors and Affiliations

  1. Plant Tissue and Organ Culture Laboratory, Department of Biotechnology, Kakatiya University, Warangal, Telangana, 506009, India

    Chaitanya Gopu, Pavani Chirumamilla, Srikanth Kagithoju & Shasthree Taduri

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  1. Chaitanya Gopu
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  2. Pavani Chirumamilla
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  3. Srikanth Kagithoju
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  4. Shasthree Taduri
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Contributions

CG and PC conducted the experiment and analyzed the data, SK designed tables and figures, and ST designed the manuscript and finalized manuscript. All authors have read and approved the final manuscript.

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Correspondence to Shasthree Taduri.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Significance’ statement

AgNPs are simple and environmentally friendly; the process is very low or cost effective and has enormous applications in the fields of biotechnology, biomedical, agriculture and environment. It has been demonstrated and characterized using a combination of different methods such as UV–Vis spectroscopy, SEM, XRD, and FTIR. The surface Plasmon structure of AgNPs was confirmed. AgNPs have shown antibacterial activity against multidrug-resistant human pathogens.

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Gopu, C., Chirumamilla, P., Kagithoju, S. et al. Green synthesis of silver nanoparticles using Momordica cymbalaria aqueous leaf extracts and screening of their antimicrobial activity. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 92, 771–782 (2022). https://doi.org/10.1007/s40011-022-01367-x

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