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Optimization of Methods for Quantification of Arjunic Acid from Bark of Terminalia arjuna from Konkan Region, India

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Abstract

Terminalia arjuna (Roxb.) Wight and Arn. is a medicinal plant distributed throughout India. It is known for wide variety of medicinal properties, especially on heart blockages. The aim of the study was to develop simple, precise, accurate, linear, robust and stability indicative HPLC method for determination of arjunic acid and also to optimize forced degradation for various stress conditions along with different solvent extractions. Method of validation was carried for the analyte and was used to evaluate arjunic acid from eight T. arjuna samples collected from Konkan corridor of Western Ghats. The analysis of various solvents showed that alcoholic solvents like isopropyl alcohol, ethanol and 90% ethanol are suitable for arjunic acid extraction than other organic solvents. However, nonpolar solvents like hexane did not produce results. Out of the samples tested for arjunic acid content, sample from Sindhudurg district showed highest accumalation of arjunic acid (0.042 ± 0.002%).

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Acknowledgements

Authors are indebted to M/S Centaur Pharmaceuticals Pvt. Ltd., Ambernath and M/S SDS lab Mahape for their technical support and extended expertise. SRP is thankful to Principal, Dada Patil Mahavidyalaya, Karjat for seed support.

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Author notes

  1. Sandeep R. Pai and Neetin S. Desai have contributed equally to this work.

Authors and Affiliations

  1. Life and Allied Sciences, Department of Bioscience, ITM University, Navi Mumbai, Maharashtra, 410201, India

    Sandip D. Desai

  2. Department of Botany, Rayat Shikshan Sanstha’s, Dada Patil Mahavidyalaya, Karjat, Dist. Ahmednagar, Maharashtra, 414402, India

    Sandeep R. Pai

  3. Sunandan Divatia School of Science, SVKM’s Narsee Monjee Institute of Management Studies, Deemed to be University (as per UGC Norms), C/O C. B. Patel Research Centre, Bhaidas Building, 3rd Floor, Bhakti Vedanta Swami Marg, Vile-Parle (West), Mumbai, Maharashtra, 400056, India

    Neetin S. Desai

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  1. Sandip D. Desai
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Correspondence to Sandeep R. Pai or Neetin S. Desai.

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Significance Statement Arjunic acid were estimated from Terminalia arjuna. Eight populations were selected for study, from Konkan corridor of Western Ghats. Alcoholic solvents were more suitable to extract arjunic acid. Elite individuals were observed in Sindhudurg district population of Konkan corridor.

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Desai, S.D., Pai, S.R. & Desai, N.S. Optimization of Methods for Quantification of Arjunic Acid from Bark of Terminalia arjuna from Konkan Region, India. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 91, 879–887 (2021). https://doi.org/10.1007/s40011-021-01264-9

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