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Spectroscopic and Microscopic Characterisation of Parotid and Submandibular Ductal Sialoliths: A Comparative Preliminary Study

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

Sialoliths, the calcified structures that develop within the salivary gland parenchyma and its ductal system, are the major cause of acute and chronic sialadenitis. Various theories of aetiology have been postulated, yet unclear. Aim of our study is to study the submandibular and parotid ductal sialoliths ultra-structurally and chemically to derive its respective mechanism of lithogenesis. Two parotid and a submandibular intraductal sialoliths were retrieved from sialadenitis patients, and each was analysed using high-resolution scanning electron microscope, energy-dispersive X-ray spectroscopy (EDS) and Fourier transform infrared (FTIR) spectroscopy and compared with each other. Scanning electron microscopic analysis of parotid sialoliths revealed irregular scaly pattern of rudely spike-like crystals with tubular porosities which might suggest earlier harbour of bacilli, whereas submandibular sialolith revealed globular, roughly rhombohedral crystals with spherical intercrystal porosities. From EDS analyses, it was evident that levels of calcium and phosphorus found to be three times more in submandibular sialolith than parotid sialolith. FTIR spectroscopic analysis showed more hydroxyl vibration spectra in submandibular sialolith, whereas parotid sialolith showed more amide and CO stretching. Above results suggest that the submandibular sialoliths are composed mainly of inorganic contents while parotid sialoliths predominantly with organic contents. Henceforth, chronic sialadenitis and morpho-anatomical disadvantage of ductal orientation plays a major role in the submandibular sialolith formation while organic nidus in the parotid sialolithogenesis.

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References

  1. Jaya Chandran S (2010) Submandibular sialoithiasis. Analysis of 4 case reports. J Int Med Sci Acad 23:97

    Google Scholar 

  2. Kraaij S, Karagozoglu KH, Forouzanfar T, Veerman EC, Brand HS (2014) Salivary stones: symptoms, aetiology, biochemical composition and treatment. Br Dent J 217:E23

    Article  CAS  Google Scholar 

  3. Jayachandran S, Bakyalakshmi K (2011) Giant submandibular sialolith presenting with sialo-cutaneous and sialo-oral fistula: a case report and review of literature. J Indian Acad Oral Med Radiol 23(3):S491–S494

    Article  Google Scholar 

  4. Nolasco P, Anjos J, Marques J, Cabrita F, Costa EC, Mauricio A et al (2013) Structure and growth of sialoliths: computed microtomography and electron microscopy investigation of 30 specimens. Microsc Microanal 19(5):1190–1203

    Article  CAS  Google Scholar 

  5. Jayasree RS, Gupta AK, Vivek V, Nayar VU (2008) Spectroscopic and thermal analysis of a submandibular sialolith of Wharton’s duct resected using Nd:YAG laser. Lasers Med Sci 23:125–131

    Article  CAS  Google Scholar 

  6. Hiraide N (1978) Morphological studies on salivary calculi. Jibiinkou 50:241–248

    Google Scholar 

  7. Mishima H, Yamamoto H, Sakae T (1992) Scanning electron microscopy-energy dispersive spectroscopy and X-ray diffraction analyses of human salivary stones. Scanning Microsc 6(2):487–494

    CAS  PubMed  Google Scholar 

  8. Grases F, Santiago C, Simonet BM, Costa-Bauz AA (2003) Sialolithiasis: mechanism of calculi formation and etiologic factors. Clin Chim Acta 334:131–136

    Article  CAS  Google Scholar 

  9. Kasaboglu O, Er N, Tumer C, Akkocaoglu M (2004) Micromorphology of sialoliths in submandibular salivary gland: a scanning electron microscope and X-ray diffraction analysis. J Oral Maxillofac Surg 62:1253–1258

    Article  Google Scholar 

  10. Sabot JF, Gustin MP, Delahougue K, Faure F, Machon C, Hartmann DJ (2012) Analytical investigation of salivary calculi, by mid-infrared spectroscopy. Analyst 137:2095–2100

    Article  CAS  Google Scholar 

  11. Harrison JD (2009) Causes, natural history, and incidence of salivary stones and obstructions. Otolaryngol Clin N Am 42:927–994

    Article  Google Scholar 

Download references

Acknowledgements

We would like to thank Sophisticated Analytical Instrument Facility (SAIF) centre, Indian Institute of Technology, Madras (HR-SEM with EDS) and Department of Medical Physics, Anna University, College of Engineering, Guindy campus, Chennai (FTIR), for providing the required facilities during the course of work.

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Authors and Affiliations

  1. Department of Oral Medicine and Radiology, Tamil Nadu Government Dental College and Hospital (Affiliated to The Tamil Nadu Dr. MGR Medical University), No. 1, Frazer Bridge Road, Muthuswamy Salai, Opposite to Fort Station, Chennai, Tamil Nadu, 600003, India

    Jayachandran Sadaksharam & Shilpa Shree Kuduva Ramesh

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  1. Jayachandran Sadaksharam
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  2. Shilpa Shree Kuduva Ramesh
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Correspondence to Shilpa Shree Kuduva Ramesh.

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Significance Statement Sialolith is the common causative for obstructive salivary gland disease which in turn is one of the most common aetiological factors of sialadenitis, a painful condition of salivary glands. Various theories have been put forth regarding lithogenesis, but unclear. Hence, there is a need to study the sialolith ultra-structurally to figure out the difference between its formation in parotid and submandibular salivary glands.

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Sadaksharam, J., Kuduva Ramesh, S.S. Spectroscopic and Microscopic Characterisation of Parotid and Submandibular Ductal Sialoliths: A Comparative Preliminary Study. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 1165–1171 (2020). https://doi.org/10.1007/s40011-020-01189-9

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  • DOI: https://doi.org/10.1007/s40011-020-01189-9

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