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Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH

Abstract

The Glossogobius giuris (Gobiidae), tank goby—a species of goby fish, is native mainly to freshwater and estuaries and has importance in the aquarium trade. It is classified as least concern under IUCN Red List. Live individuals of this species were collected from Loktak Lake in Manipur for cytogenetic investigation to reveal the nucleolar organizer region (NOR) through silver nitrate (AgNO3) and chromomycin A3 (CMA3) staining as well as DAPI staining and single-color fluorescence in situ hybridization (FISH) using 18S rDNA sequence as probe. Analysis of more than 50 metaphase chromosome complement (obtained from colchicine–potassium chloride–Carnoy’s fixation–Giemsa staining procedures) showed the presence of 46 diploid chromosome numbers with all telocentric having fundamental arm number as 46 without any heteromorphic pair. One pairs of silver-stained NORs, situated terminally on the telocentric chromosome, were observed. Similarly, one pair of CMA3-positive sites were observed on the chromosome that suggested abundance of GC-rich repetitive DNAs in this region. One pair of 18S rDNA positive sites was observed on the telocentric chromosomes using FISH. These karyological features can be useful markers in cytotaxonomy and conservation of this species.

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References

  1. Mariano CDSF, Pompolo SDG, Silva JG, Delabie JHC (2012) Contribution of cytogenetics to the debate on the paraphyly of Pachycondyla spp. (Hymenoptera, Formicidae, Ponerinae). Psyche. https://doi.org/10.1155/2012/973897

    Article  Google Scholar 

  2. Kumar R, Baisvar VS, Kushwaha B, Waikhom G, Nagpure NS (2017) Cytogenetic investigation of Cyprinus carpio (Linnaeus, 1758) using giemsa, silver nitrate, CMA3 staining and fluorescence in situ hybridization. Nucleus 60:1–8

    CAS  Article  Google Scholar 

  3. Kushwaha B, Srivastava SK, Nagpure NS, Ogale SN, Ponniah AG (2001) Cytogenetic studies of Mahaseer, Tor khudree and Tor mussullah (Cyprinidae, Pisces) from India. Chromosome Sci 5:47

    Google Scholar 

  4. Talwar PK, Jhingran AG (1991) Inland fishes of India and adjacent countries, vol I. Oxford and IBH Publishing Co., New Delhi

    Google Scholar 

  5. Bertollo LAC, Takahashi CS, Moreira-Filho O (1978) Cytotaxonomic consideration on Hoplias lacerdae (Pisces Erythrinidae). Braz J Genet 1:103

    Google Scholar 

  6. Levan A, Fredga KY, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201

    Article  Google Scholar 

  7. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014

    CAS  Article  Google Scholar 

  8. Ueda T, Irie S, Kato Y (1987) Longitudinal differentiation of metaphase chromosomes of Indian muntjac as studies by restriction enzyme digestion, in situ hybridization with cloned DNA probes and distamycin a plus DAPI fluorescence staining. Chromosoma 95:251–257

    CAS  Article  Google Scholar 

  9. Sambrook J, Russell I (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Plainsveiw

    Google Scholar 

  10. Winterfeld G, Roser M (2007) Deposition of ribosomal DNAs in the chromosome of perennial oats (Poaceae: Aveneae). Bot J Linn Soc 155:193–210

    Article  Google Scholar 

  11. Masagca JT, Ordonez JA (2003) Karyomorphology of the Philippine rock goby, Glossogobius giuris (gobiidae) from Lake Taal and some rivers of Cavite, Luzon Island. Biotropia 2:11–18

    Google Scholar 

  12. Manna GK, Prasad R (1974) Chromosome analysis in three species of fishes belonging to family Gobitidae. Cytologia 39:609–618

    CAS  Article  Google Scholar 

  13. Arkhipchuk, VV (1999) Chromosome database. Database of Dr. Victor Arkhipchuk. https://www.fishbase.de/references/FBRefSummary.php?ID=30184&win=eli. Accessed 15 Mar 2018

  14. Vasil’ev VP (1980) Chromosome numbers in fish-like vertebrates and fish. J Ichthyol 20:1–38

    Google Scholar 

  15. Kaur D, Srivastava MDC (1965) The structure and behaviour of chromosome in five freshwater teleosts. Caryologia 18:181–191

    Article  Google Scholar 

  16. Rishi KK, Singh J (1982) Karyological studies on five estuarine fishes. Nucleus 25:178–180

    Google Scholar 

  17. Klinkhardt M, Tesche M, Greven H (1995) Database of fish chromosomes. Westarp Wissenschaften. https://www.fishbase.de/references/FBRefSummary.php?ID=34370&win=eli. Accessed 15 Mar 2018

  18. Prasad R (1971) Not provided. PhD thesis, Kalyani University, Kalyani, W.B

  19. Jankun M, Oclaewicz K, Pardo BG, Martinez P et al (2003) Localization of 5SrRNA loci in three coregonid species (Samonidae). Genetica 119:183

    CAS  Article  Google Scholar 

  20. Rincao MP, Chavari JL, Brescovit AD, Dias AL (2017) Cytogenetic analysis of five Ctenidae species (Araneae): detection of heterochromatin and 18S rDNA sites. Comp Cytogenet 11:627–639

    Article  Google Scholar 

  21. Jordan G (1987) At the heart of nucleolus. J Nat 329:489–490

    CAS  Article  Google Scholar 

  22. Vitturi R, Colomba MS, Barbieri R, Zunino M (1999) Ribosomal DNA location in the scrab beetle Thorectes intermedius (costa) (Coleopterea: Geotrupidae) using banding and fluorescent in situ hybridization. Chrom Res 7:255

    CAS  Article  Google Scholar 

  23. Klinkhardt MB (1998) Some aspect of karyoevolution in fishes. Ani Res Dev 47:7

    Google Scholar 

  24. Zhang D, Sang T (1999) Physical mapping of ribosomal RNA genes in Peonies (Paenia, Paeoniaceae) by fluorescent in situ hybridization: implications for phylogeny and concerted evolution. Am J Bot 86:735–740

    CAS  Article  Google Scholar 

  25. Jiang J, Gill BS (1994) Nonisotopic in situ hybridization and plant genome mapping: the first 10 years. Genome 37:717–725

    CAS  Article  Google Scholar 

  26. Das JK, Khuda-Bukhsh AR (2007) GC-rich heterochromatin in silver stained nucleolar organizer regions (NORs) fluoresces with chromomycin A3 (CMA3) staining in three species of teleostean fishes (Pisces). Indian J Exp Biol 45:413–418

    CAS  PubMed  Google Scholar 

  27. Kumar R, Kushwaha B, Nagpure NS, Behera BK, Srivastava SK, Lakra WS (2009) Physical mapping of rRNA gene in endangered fish Osteobrama belangeri (Valenciennes, 1844) (Family: Cyprinidae). Indian J Exp Biol 47:597–601

    CAS  PubMed  Google Scholar 

  28. Siljak-Yakovlev S, Godelle B, Zoldos V, Valles J, Garnatje T, Hidalgo O (2017) Evolutionary implications of heterochromatin and rDNA in chromosome number and genome size changes during dysploidy: a case study in Reichardia genus. PLoS ONE 12:1–21

    Article  Google Scholar 

  29. Singh M, Kumar R, Nagpure NS, Kushwaha B, Mani I, Chauhan UK, Lakra WS (2009) Population distribution of 45S and 5S rDNA in golden mahseer, Tor Putitora: population-specific FISH marker. J Genet 88:315–320

    CAS  Article  Google Scholar 

  30. Silva GS, Souza MM, de Melo CAF, Urdampilleta JD, Forni-Martins ER (2018) Identification and characterization of karyotype in Passiflora hybrids using FISH and GISH. BMC Genet 19:26–36

    CAS  Article  Google Scholar 

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Acknowledgements

The authors are grateful to the Director, ICAR- National Bureau of Fish Genetic Resources, Lucknow, for his support and encouragement for taking up this research program. They are thankful to the Department of Biotechnology, Ministry of Science and Technology, Government of India, for financial support through Project Sanction No. BT/13/NE/TBP/2010 dated March 16, 2011, and October 20, 2011.

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Correspondence to Ravindra Kumar.

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Significance Statement Tank goby, G. giuris, has world-wide distribution and importance in aquarium trade. The basic cytogenetic information, except diploid chromosome number, is lacking in the species. The information generated will be useful in cytotaxonomic identification as well as conservation and management of this species.

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Kumar, R., Baisvar, V.S., Kushwaha, B. et al. Cytogenetic Studies in Glossogobius giuris (Hamilton, 1822) Through NOR-Staining and FISH. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 221–226 (2020). https://doi.org/10.1007/s40011-019-01084-y

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Keywords

  • Glossogobius giuris
  • Giemsa
  • AgNOR
  • CMA3
  • FISH
  • 18S rDNA