Skip to main content
SpringerLink
Log in

Characterization, Antimicrobial and α-Amylase Inhibitory Activity of Silver Nanoparticles Synthesized by using Mushroom Extract of Lentinus tuber-regium

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The present investigation was conducted to synthesize and characterize silver nanoparticles using wild edible mushroom extract of Lentinus tuber-regium. The current investigation also aims to determine antimicrobial and α-amylase inhibitory activity of biosynthesized silver nanoparticles (AgNPs). UV–Visible spectrophotometer study indicated that the surface plasmon resonance peak of reaction of AgNPs was around at 433 nm which confirmed the formation of AgNPs. Fourier-transform infrared spectroscopy analysis confirms that carboxyl functional groups in mushroom extract are mainly responsible in reduction of Ag+ ions to Ag0 nanoparticles. Atomic force microscopy, transmission electron microscopy and scanning electron microscopy analysis revealed that synthesized AgNPs were spherical and have an average size within 5 to 35 nm. Selected area electron diffraction and X-ray diffraction pattern displayed that synthesized AgNPs were crystalline in nature. Energy-dispersive X-ray spectroscopy study showed 10.50% of silver metal in weight in the sample. Biosynthesized AgNPs showed more antibacterial activity in Gram (−) bacteria than Gram (+). Minimum inhibitory concentration values of the biosynthesized AgNPs against Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus and Bacillus subtilis were found to be 9, 12, 14 and 13 µg/ml, respectively. Inhibition of α-amylase activity increased with increasing concentration of biosynthesized AgNPs. This work substantially indicates that mushroom is efficient in biosynthesis of silver nanoparticles having potential antimicrobial and α-amylase inhibitory activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Banerjee K, Rai VR (2016) Study on green synthesis of gold nanoparticles and their potential applications as catalysts. J Clust Sci 27:1307–1315

    CAS  Google Scholar 

  2. Sujatha S, Tamilselvi S, Subha K (2013) Panneerselvam A (2013) On biosynthesis of silver nanoparticles using mushroom and its antibacterial activities. Int J Curr Microbiol Appl Sci 2(12):605

    Google Scholar 

  3. Song JY, Kim BS (2009) Rapid biological synthesis of silver nanoparticles using plant leaf extracts. Bioprocess Biosyst Eng 32:79–84

    PubMed  Google Scholar 

  4. Rajasekharreddy P, Rani PU, Sreedhar B (2010) Qualitative assessment of silver and gold nanoparticle synthesis in various plants: a photobiological approach. J Nanoparticle Res 12:1711–1721

    CAS  Google Scholar 

  5. Kumar V, Yadav SK (2009) Plant-mediated synthesis of silver and gold nanoparticles and their applications. J Chem Technol Biotechnol 84:151–157

    CAS  Google Scholar 

  6. Khatoon N, Mazumder JA, Sardar M (2017) Biotechnological applications of green synthesized silver nanoparticles. J Nanosci Curr Res 2:1–8

    Google Scholar 

  7. Sreekanth TVM, Lee KD (2011) Green synthesis of silver nanoparticles from Carthamus tinctorius flower extract and evaluation of their antimicrobial and cytotoxic activities. Curr Sci 7:1046–1053

    CAS  Google Scholar 

  8. Debnath G, Dutta S, Saha AK, Das P (2016) Green synthesis, characterization and antibacterial activity of silver nanoparticles (Silver nanoparticles) from grass leaf extract Paspalum conjugatum P.J. Berguis. J Mycopathol Res 54(3):371–376

    Google Scholar 

  9. Ahmad R, Shahverdi SM, Shahverdi HR, Hossein J, Nohi AA (2007) Rapid synthesis of silver nanoparticles using culture supernatants of Enterobacteria. Biochem 42:919–923

    Google Scholar 

  10. Bilal D, Gurunathan S (2008) Biosynthesis of silver nanocrystals by Bacillus licheniformis. Colloids Surf B 65:150

    Google Scholar 

  11. Bhattacharjee S, Debnath G, Roy Das A, Saha AK, Das P (2017) Characterization of silver nanoparticles synthesized using an endophytic fungus, Penicillium oxalicum having potential antimicrobial activity. Adv Nat Sci Nanosci Nanotech 8:1–6

    Google Scholar 

  12. Roh Y, Lauf RJ, Mcmillan AD, Zhang C, Rawn CJ, Bai J, Phelps TJ (2001) Microbial synthesis and the characterization of metal-substituted magnetites. Solid State Commun 118:529–534

    CAS  Google Scholar 

  13. Mukherjee P, Ahmad A, Mandal D, Senapati S, Sainkar SR, Khan MI, Ramani R, Parischa R, Kumar PAV, Alam Sastry MMK, Angew M (2001) Bioreduction of AuCl4− ions by the Fungus, Verticillium sp. and surface trapping of the gold nanoparticles. Chem Int Ed 40:3585–3588

    CAS  Google Scholar 

  14. Philip D (2009) Biosynthesis of Au, Ag and Au-Ag nanoparticles using edible mushroom extract. Spectrochem Acta A Mol Biomol Spectrosc 73(2):374–381

    Google Scholar 

  15. Nithya R, Ragunathan R (2009) Synthesis of silver nanoparticle using Pleurotus sajorcaju and its antimicrobial study. Digest J Nanomater Biostrut 4:623–629

    Google Scholar 

  16. Karwa A, Gaikwad S, Rai MK (2011) Mycosynthesis of silver nanoparticles using Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt.: Fr.) P. Karst. and their role as antimicrobials and antibiotic activity enhancers. Int J Med Mushrooms 13:483–489

    PubMed  CAS  Google Scholar 

  17. Narasimha G, Praveen B, Mallikarjuna K, Raju BDP (2011) Mushrooms (Agaricus bisporus) mediated biosynthesis of sliver nanoparticles, characterization and their antimicrobial activity. Nano Dimens 2(1):29–36

    CAS  Google Scholar 

  18. Nagajyothi PC, Sreekanthb J, Leec TVM, Leec KD (2014) Mycosynthesis: antibacterial, antioxidant and antiproliferative activities of silver nanoparticles synthesized from Inonotus obliquus (Chaga mushroom) extract. J Photochem Photobiol B Biol 130:299–304

    CAS  Google Scholar 

  19. Sheikhloo Z, Salouti M (2012) Intracellular biosynthesis of gold nanoparticles by fungus Phoma macrostoma. Synth React Inorg Met Org Nano-Met Chem 42:65–67

    CAS  Google Scholar 

  20. Awwad AM, Salem NM, Abdeen AO (2013) Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. Intern J Ind Chem 4:29–35

    Google Scholar 

  21. Lansdown ABG (2010) Silver in healthcare: its antimicrobial efficacy and safety in use. R Soc Chem 159

  22. Dannis S, Dhruba B (2011) The role of nanotechnology in diabetes treatment: current and future perspectives. Int J Nanotechnol 8(1):53–65

    Google Scholar 

  23. Daisy P, Saiprya K (2012) Biochemical analysis of Cassia fistula aqueous extract and phytochemically synthesized gold nanoparticles as hypoglycemic treatment for diabetes mellitus. Int J Nanomed 7:1189–1202

    CAS  Google Scholar 

  24. Pegler D (1977) A preliminary agaric flora of East Africa. Kew Bull. Addit. Ser. 6:1–615

    Google Scholar 

  25. Ul-Haq M, Rathod V, Singh D, Singh AK, Ninganagouda S, Hiremath J (2015) Dried Mushroom Agaricus bisporus mediated synthesis of silver nanoparticles from Bandipora District (Jammu and Kashmir) and their efficacy against Methicillin Resistant Staphylococcus aureus (MRSA) strains. Nanosci and Nanotechnol Int J 5(1):1–8

    Google Scholar 

  26. Bauer AW, Kirby WM, Sherris JC, Turck M (1966) Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45:493–496

    PubMed  CAS  Google Scholar 

  27. Nickavara B, Yousefiana N (2009) Inhibitory effects of six Allium species on α-amylase enzyme activity. Iran J Pharm Res 8(1):53–57

    Google Scholar 

  28. Mulvaney P (1996) Surface plasmon spectroscopy of nanosized metal particles. Langmuir 12:788–800

    CAS  Google Scholar 

  29. Akanna S, Prasad KV, Elumalai EK, Savithramma N (2010) Production of biogenic silver nanoparticles using Boswellia ovalifoliolata stem bark. Digest J Nanomater Biostruct 5(2):369–372

    Google Scholar 

  30. Singh RP, Shukla VK, Yadav RS, Sharma PK, Singh PK, Pandey AC (2011) Biological approach of zinc oxide nanoparticles formation and its characterization. Adv Mater Lett 2:313–317

    Google Scholar 

  31. Lee KD, Kuppusamy P, Kim DH, Govindan N, Maniam GP, Choi KC (2018) Forage crop Lolium multiflorum Assisted synthesis of AgNPs and their bioactivities against poultry pathogenic bacteria in in vitro. Indian J Microbiol 58(4):507–514

    PubMed  PubMed Central  CAS  Google Scholar 

  32. Bhat R, Deshpande R, Ganachari SV, SungHuh D, Venkataraman A (2011) Photo-irradiated biosynthesis of silver nanoparticles using edible mushroom Pleurotus florida and their antibacterial activity studies. Bio Chem Appl 2011:1–7

    Google Scholar 

  33. Chen Q, Liu G, Chen G, Mi T, Tai J (2017) Green synthesis of silver nanoparticles with glucose for conductivity enhancement of conductive ink. BioRes 12(1):608–621

    CAS  Google Scholar 

  34. Gong P, Li H, He X, Wang K, Hu J, Tan W (2007) Preparation and antibacterial activity of Fe3O4 Ag nanoparticles. Nanotechnology 18:604–611

    Google Scholar 

  35. Yugandhar P, Haribabu R, Savithramma N (2015) Synthesis, characterization and antimicrobial properties of green-synthesised silver nanoparticles from stem bark extract of Syzygium alternifolium (Wt.) Walp. 3 Biotech 5:1031–1039

    PubMed  PubMed Central  Google Scholar 

  36. Yugandhar P, Savithramma N (2016) Biosynthesis, characterization and antimicrobial studies of green synthesized silver nanoparticles from fruit extract of Syzygium alternifolium (Wt.) Walp. an endemic, endangered medicinal tree taxon. Appl Nanosci 6:223–233

    CAS  Google Scholar 

  37. Singha G, Bhavesh R, Kasariya K, Sharma AR, Singh RP (2011) Biosynthesis of silver nanoparticles using Ocimum sanctum (Tulsi) leaf extract and screening its antimicrobial activity. J Nanoparticle Res 13:2981–2988

    Google Scholar 

  38. Otari SV, Patil RM, Nadaf NH, Ghosh SJ, Pawar SH (2014) Green synthesis of silver nanoparticles by microorganism using organic pollutant: its antimicrobial and catalytic application. Environ Sci Pollut Res 21:1503–1513

    CAS  Google Scholar 

  39. Hungund B, Gayatri S, Dhulappanavar R, Ayachit NH (2015) Comparative evaluation of antibacterial activity of silver nanoparticles biosynthesized using fruit juices. J Nanomed Nanotechnol 6(2):1–6

    Google Scholar 

  40. Kuppusamy P, Yusoff MM, Maniam GP, Govindan N (2014) Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications—an updated report. Saudi Pharm J 24:473–484

    PubMed  PubMed Central  Google Scholar 

  41. Khair-ul-Bariyah S, Ahmed D, Aujla MI (2012) Comparative Analysis of Ocimum basilicum and Ocimum sanctum: extraction techniques and urease and alpha-amylase inhibition. Pak J Chem 2(3):1–8

    Google Scholar 

  42. Aruna A, Nandhini R, Karthikeyan V, Bose P, Vijayalakshmi K (2014) Comparative anti-diabetic effect of methanolic extract of insulin plant (Costus pictus) leaves and its silver nanoparticles. Indo Am J Pharm Res 4:3217–3230

    Google Scholar 

Download references

Acknowledgements

The authors are grateful to the Head, Department of Botany, Tripura University, for providing facilities. The first author is grateful to UGC, Government of India, for BSR fellowship.

Author information

Authors and Affiliations

  1. Mycology and Plant Pathology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, 799022, India

    Gopal Debnath & Ajay Krishna Saha

  2. Microbiology Laboratory, Department of Botany, Tripura University, Suryamaninagar, Tripura, 799022, India

    Panna Das

Authors
  1. Gopal Debnath
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Panna Das
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ajay Krishna Saha
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Gopal Debnath.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest to publish this manuscript.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Significance Statement

Wild edible mushroom Lentinus tuber-regium can be used as a source of reducing and capping agents of spherical metallic silver nanoparticles with average size 5–35 nm, and synthesized AgNPs using the mushroom offer potential medicinal properties.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Debnath, G., Das, P. & Saha, A.K. Characterization, Antimicrobial and α-Amylase Inhibitory Activity of Silver Nanoparticles Synthesized by using Mushroom Extract of Lentinus tuber-regium. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 90, 37–45 (2020). https://doi.org/10.1007/s40011-019-01076-y

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-019-01076-y

Keywords

Search

Navigation