Skip to main content
Log in

An Inverse Correlation Between the Production of Itaconic and Mevinolinic Acids in Aspergillus terreus Mutants

  • Research Article
  • Published:
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

The high producer A. terreus ATWD 136 and the world A. terreus FRR 5360 wild strains were used to improve their productivity by physical mutagenesis. The production was decreased in all the mutants obtained from the mutagenesis of the FRR 5360 strain, while the mutants obtained from the ATWD 136 strain showed different responses in the production. Thus, they were grouped in two categories according to IA/MA production. The first category (three mutants) revealed that UV-irradiation-enhanced IA production, while the production of MA was decreased. The second category (14 mutants) showed an enhancement obtained in the MA production, while the production of IA was decreased. The mutant 16 was the best IA producer (threefold higher than the ATWD 136 parent strain), and the mutant 12 was the best MA producer (twofold higher than the ATWD 136 parent strain). Finally, RAPD–PCR confirmed the presence of different value of genetic variability between the wild A. terreus ATWD 136 and its hyper-producing mutants (M 12 and M 16). From the results, the authors concluded that there is an inverse correlation between level yield of IA and MA in mutated A. terreus strains.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Meena V, Sumanjali A, Dwarka K, Subburathinam K, Sambasiva Rao K (2010) Production of itaconic acid through submerged fermentation employing different species of Aspergillus. Rasayan J Chem 16:100–109

    Google Scholar 

  2. Samiee SM, Moazami N, Haghighi S, Aziz Mohseni F, Mirdamadi S, Bakhtiari MR (2003) Screening of lovastatin production by filamentous fungi. Iran Biomed J 7:29–33

    CAS  Google Scholar 

  3. Willke T, Vorlop KD (2001) Biotechnological production of itaconic acid. Appl Microbiol Biotechnol 56:289–295

    Article  CAS  Google Scholar 

  4. Okabe M, Lies D, Kanamasa S, Park EY (2009) Biotechnological production of itaconic acid and its biosynthesis in Aspergillus terreus. Appl Microbiol Biotechnol 84(4):597–606

    Article  CAS  Google Scholar 

  5. Hasan HAH, Elfarash AE, Abdrabo KhAE (2016) Aspergillus terreus occurrence in middle of Upper Egypt, genetic variation and production of itaconic and mevinolinic acids. Eur J Biol Res 6(4):242–253

    CAS  Google Scholar 

  6. Vinci V, Hoerner T, Coffman A, Schimmel T, Dabora R, Kirpekar A, Ruby C, Stieber R (1991) Mutants of a lovastatin-hyperproducing Aspergillus terreus deficient in the production of sulochrin. J Ind Microbiol 8(2):113–119

    Article  CAS  Google Scholar 

  7. Shakibaie M, Ameri A, Ghazanfarian R, Adeli-Sardou M, Amirpour-Rostami S, Torkzadeh-Mahani M, Imani M, Forootanfar H (2018) Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus. Braz J Microbiol 49(4):865–871

    Article  CAS  Google Scholar 

  8. Yahiro K, Takahama T, Park YS, Okabe M (1995) Breeding of Aspergillus terreus mutant TN-484 for itaconic acid production with high yield. J Ferment Bioeng 79:506–508

    Article  CAS  Google Scholar 

  9. Kumar MS, Kumar PM, Sarnaik HM, Sadhukhan A (2000) A rapid technique for screening of lovastatin-producing strains of Aspergillus terreus by agar plug and Neurospora crassa bioassay. J Microbiol Methods 40:99–104

    Article  CAS  Google Scholar 

  10. Ferrón MV, Lopez JC, Perez JS, Sevilla JF, Chisti Y (2005) Rapid screening of Aspergillus terreus mutants for overproduction of lovastatin. World J Microbiol Biotechnol 21:123–125

    Article  Google Scholar 

  11. Balajee SA, Gribskov JL, Hanley E, Nickle D, Marr KA (2005) Aspergillus lentulus sp. nov., a new sibling species of A. fumigatus. Eukaryot Cell 4:625–632

    Article  CAS  Google Scholar 

  12. Hong SB, Shin HD, Hong J, Frisvad JC, Nielsen PV, Varga J, Samson RA (2008) New taxa of Neosartorya and Aspergillus in Aspergillus section Fumigati. Antonie Van Leeuwenhoek 93:87–98

    Article  Google Scholar 

  13. Sanger F, Nicklen S, Coulson A (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci 74:5463–5467

    Article  CAS  Google Scholar 

  14. Prabhakar M, Lingappa K (2011) Screening of A. terreus Klvb28 mutants by bioassay method for overproduction of lovastatin (a vital statin in coronary heart disease). Res J Biotech 6(3):33–35

    CAS  Google Scholar 

  15. Irum W, Anjum T (2012) Production enhancement of cyclosporin ‘A’by Aspergillus terreus through mutation. Afr J Biotechol 11:1736–1743

    CAS  Google Scholar 

  16. Hartford CG (1962) Rapid spectrophotometric method for the determination of itaconic, citric, aconitic, and fumaric acids. Anal Chem 34:426–428

    Article  CAS  Google Scholar 

  17. López JC, Pérez JS, Sevilla JF, Fernandez FA, Grima EM, Chisti Y (2003) Production of lovastatin by Aspergillus terreus: effects of the C: N ratio and the principal nutrients on growth and metabolite production. Enzym Microb Technol 33:270–277

    Article  Google Scholar 

  18. Lingappa K, Babu CV, Siddalingeshwar K, Pramod T (2004) Isolation, screening and rapid confirmation of lovastatin producing strains of Aspergillus terreus. Indian J Microbiol 44:133–136

    CAS  Google Scholar 

  19. Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard R (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci 81:8014–8018

    Article  CAS  Google Scholar 

  20. Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci 76:5269–5273

    Article  CAS  Google Scholar 

  21. Jaivel N, Marimuthu P (2010) Optimization of lovastatin production in solid state fermentation by Aspergillus terreus. Int J Eng Sci Technol 2:2730–2733

    Google Scholar 

  22. Kariya M, Fujiwara H (1994) Manufacture of itaconic acid with heat resistant Aspergillus terreus. JP-Patent 6:038-774

  23. Qari SH (2008) Molecular and biochemical evaluation of genetic effect of Calotropis procera (Ait.) latex on Aspergillus terreus (Thom). Indian J Exp Biol 46:725

    CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors wish to acknowledge Assiut University for providing all necessary facilities during the research period.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Khaled A. E. Abdrabo.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest to publish this manuscript.

Additional information

Significance StatementAspergillus terreus was known to be the best species to produce itaconic and mevinolinic acids. Itaconic acid is an important organic acid with a lot of industrial applications. Mevinolinic acid is a drug that lowers the level of endogenous cholesterol in human blood serum.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Hasan, H.A.H., Elfarash, A.E. & Abdrabo, K.A.E. An Inverse Correlation Between the Production of Itaconic and Mevinolinic Acids in Aspergillus terreus Mutants. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 1231–1237 (2019). https://doi.org/10.1007/s40011-018-1041-6

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s40011-018-1041-6

Keywords

Navigation