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Evaluating Oxidative Stress in Labeo rohita, Infected Asypmtomatically with Native and Invasive Aeromonads Using Biochemical Indices

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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Aims and scope Submit manuscript

Abstract

In view of the importance of disease management in aquaculture, the impacts of Aeromonas hydrophila and Aeromonas salmonicida infection at sublethal dose on Labeo rohita (Hamilton, 1822), were investigated by determining the changes in the levels of serum biochemical parameters (total protein, glucose, cholesterol, bilirubin and calcium); detoxifying enzyme concentration {glutathione peroxidase (GPx), glutathione reductase (GR) and glutathione (GSH)} and rate of reactive oxygen species production in phagocytes. Fishes were inoculated (i.p.) with 3 × 107 cfu ml−1 of A. hydrophila and 2 × 107 cfu ml−1 of A. salmonicida to generate asymptomatic carrier state. After an exposure period of 3 days, the above parameters were studied. The levels of total protein, glucose, cholesterol, bilirubin and calcium concentration in serum were found to be significantly (p < 0.05) higher in infected fishes. Total protein level was not significantly different among the infected groups. However, glucose and cholesterol concentrations were higher in A. salmonicida infected fish while cholesterol and calcium levels were higher in A. hydrophila treated group. Concentrations of all the detoxifying enzymes in A. hydrophila treated fishes were higher than those measured in A. salmonicida treated group and control fishes. The rate of reactive oxygen production in phagocytes was determined by formazan deposition which indicated that A. salmonicida infection triggered more reactive oxygen as compared to A. hydrophila treatment. The findings indicated that these parameters could be analyzed as potential exposure biomarkers for clinical monitoring of fish health at field level against pathogenic Aeromonads.

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References

  1. Saksida S, Constantine J, Karreman GA, Neville C, Sweeting R, Beamish R (2006) Evaluation of sea lice, Lepeophtheirus salmonis, abundance levels on farmed salmon in British Columbia, Canada. In: Proceedings from the international symposium on veterinary epidemiology and economics XI

  2. Kaleeswaran B, Iavenil S, Ravikumar S (2012) Changes in biochemical, histological and specific immune parameters in Catla catla (Ham) by Cynodon dactylon (L.). J King Saud Univ Sci 24(2):139–152

    Article  Google Scholar 

  3. Almeida A, Cunha Â, Gomes N, Alves E, Costa L, Faustino MA (2009) Phage therapy and photodynamic therapy: low environmental impact approaches to inactivate microorganisms in fish farming plants. Mar Drugs 7(3):268–313

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Harikrishnan R, Balasundaram C, Bhuvaneswari R (2005) Restorative effect of Azadirachta indica aqueous leaf extract dip treatment on haematological parameter changes in Cyprinus carpio (L.) experimentally infected with Aphanomyces invadans fungus. J Appl Ichthyol 21(5):410–413

    Article  Google Scholar 

  5. Tellez-Bañuelos MC, Santerre A, Casas Solis J, Zaitseva G (2010) Endosulfan increases seric interleukin-2 like (IL-2L) factor and immunoglobulin M (IgM) of Nile tilapia (Oreochromis niloticus) challenged with Aeromonas hydrophila. Fish Shellfish Immunol 28(2):401–405

    Article  CAS  PubMed  Google Scholar 

  6. Bernoth EM, Ellis AE, Midtlyng PJ, Olivier G, Smith P (1997) Furunculosis. Multidisciplinary fish disease research, vol 10. Academic Press Ltd., London, pp 248–268

    Google Scholar 

  7. Pal S, Datta RS, Homechaudhuri S (2015) Evaluation of in vivo non-specific immunity and oxidative stress in Labeo rohita (Hamilton, 1822) infected with Aeromonas hydrophila as biomarker for early diagnosis. Int J Fish Aquat Stud 3(1):116–124

    Google Scholar 

  8. Pal S, Datta RS, Homechaudhuri S (2016) Serum bactericidal activity as indicator of innate immunity in Labeo rohita (Hamilton, 1822) challenged with Aeromonas hydrophila as biomarker for clinical monitoring. Int J Adv Res Biol Sci 3(1):134–144

    CAS  Google Scholar 

  9. Pal J, Pradhan K (1990) Bacterial involvement in ulcerative condition of air-breathing fish from India. J Fish Biol 36(6):833–839

    Article  Google Scholar 

  10. Gómez-Ochoa P, Sabateb D, Homedesb J, Ferrerc L (2012) Use of the nitroblue tetrazolium reduction test for the evaluation of Domperidone effects on the neutrophilic function of healthy dogs. Vet Immunol Immunopathol 146:97–99

    Article  CAS  PubMed  Google Scholar 

  11. Zar JH (1999) Biostatistical analysis, 4th edn. Prentice Hall, Upper Saddle River

    Google Scholar 

  12. Davis KB, Parker NC (1990) Physiological stress in striped bass: effect of acclimation temperature. Aquaculture 91:349–358

    Article  Google Scholar 

  13. Barton BA, Iwama GK (1991) Physiological changes in the fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annu Rev Fish Dis 1:3–26

    Article  Google Scholar 

  14. Pickering AD (1993) Endocrine- induced pathology in stressed salmoid fish. Fish Res 17:35–50

    Article  Google Scholar 

  15. Citarasu T, Sivaram V, Immanuel G, Rout N, Murugan V (2006) Influence of selected Indian immunostimulant herbs against white spot syndrome virus (WSSV) infection in black tiger shrimp, Penaeus monodon with reference to haematological, biochemical and immunological changes. Fish Shellfish Immunol 21:372–384

    Article  PubMed  Google Scholar 

  16. Allen FM, Patrick JW, Roger TH (2005) Blood biochemistry of the oyster toadfish. J Aquat Anim Health 17:170–176

    Article  Google Scholar 

  17. Rehulka J (1998) Blood indices of the rainbow trout (Oncorhynchus mykiss) in Aeromonas-induced ulcerous dermatitis. Acta Vet Brno 67(3):17–322

    Google Scholar 

  18. Mastoi AM, Sukumaran M, Mastoi A, Hassan A, Shaharom F, Chatterji A (2011) Differences in haematological parameters in normal, infected and immune-primed fingerlings of Red Tilapia (Oreochromis mossambicus × Oreochromis niloticus). Biol Forum 4:90–97

    Google Scholar 

  19. Matsoff L, Oikari A (1987) Acute hyper bilirubinaemia in rainbow trout (Salmon gairdneri) caused by resin acids. Comp Biochem Physiol 88C:263–268

    Google Scholar 

  20. Bansal SK, Verma SR, Gupta AK, Dalela RC (1979) Physiological dysfunction of the haemopoietic system in a freshwater teleost, Labeo rohita following chronic chlordane exposure. Part I—alteration in certain haematological parameters. Bull Environ Contam Toxicol 22:666–673

    Article  CAS  PubMed  Google Scholar 

  21. Dalela RC, Rani S, Kumar V, Verma SR (1981) In vivo hematological alteration in a freshwater teleost Mytsus vittatus following sub-acute exposure to pesticides and their combination. J Environ Biol 2(2):79

    CAS  Google Scholar 

  22. Sastry KV, Sharma SK (1978) The effect of endrin on the histopathological changes in the liver 01 Channa punctatus. Bull Environ Contam Toxicol 20:671–677

    Google Scholar 

  23. Muniz Junqueira MI, de Paula-Coelho VN (2008) Meglumineanti-monate directly increases phagocytosis, superoxide anion and TNF-alpha production, but only via TNF-alpha it indirectly increases nitric oxide production by phagocytes of healthy individuals, in vitro. Int Immuno pharmacol 8:1633–1638

    Article  CAS  Google Scholar 

  24. Winston GW, Di Giulio RT (1991) Prooxidant and antioxidant mechanisms in aquatic organisms. Aquat Toxicol 19:137–161

    Article  CAS  Google Scholar 

  25. Zhang J, Shen H, Wang X, Wu J, Xue Y (2004) Effects of chronic exposure of 2, 4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere 55:167–174

    Article  CAS  PubMed  Google Scholar 

  26. Vieira LR, Sousa A, Frasco MF, Lima I, Morgado F, Guilhermino L (2008) Acute effects of benzo(α)pyrene, anthracene and a fuel oil on biomarkers of the common goby Pomatoschistus microps (Teleostei, Gobiidae). Sci Total Environ 395:87–100

    Article  CAS  PubMed  Google Scholar 

  27. Kaur S, Kaur A (2015) Variability in antioxidant/detoxification enzymes of Labeo rohita exposed to an azo dye, acid black (AB). Comp Biochem Physiol Part C 167:108–116

    CAS  Google Scholar 

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Acknowledgements

The authors are thankful to the Head of the Department of Zoology, University of Calcutta, India for providing the facilities. The Department of Science and Technology Scientific and Engineering Research Board funded project [SR/SO/AS-067/2013 dated 10.7.2014] is thankfully acknowledged.

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Correspondence to Sumit Homechaudhuri.

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Significance Statement

An approach towards biomarker development by multiparametric serum biochemical profiling and evaluation of oxidative stress involving non-invasive sample collection from L. rohita infected with A. hydrophila and A. salmonicida at a sublethal dose.

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Roy, D., Pal, S., Datta Ray, S. et al. Evaluating Oxidative Stress in Labeo rohita, Infected Asypmtomatically with Native and Invasive Aeromonads Using Biochemical Indices. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 89, 973–978 (2019). https://doi.org/10.1007/s40011-018-1012-y

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